FlyBase Allele Report: Dmel\Cbl[L.UAS]

General Information

Symbol

Dmel\Cbl^L.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0240613

Feature type

allele

Associated gene

Dmel\Cbl

Associated Insertion(s)

Carried in Construct

P{UAS-Cbl.L}

Also Known As

UAS-D-cblL, UAS-Cbl-L

Key Links

Transgenic product class

cDNA

(Pai et al., 2006)

wild_type

(Pai et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Pai et al., 2006)

wild_type

(Pai et al., 2006)

Mutagen

in vitro construct

(Pai et al., 2006)

Progenitor genotype

Carried in construct

P{UAS-Cbl.L}

Cytology

Description

Expression of Cbl long isoforms cDNA (GenBank:DQ485790) is under the control of UASt sequences.

(Pai et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Cbl

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of P-stage, with Scer\GAL4^e22c

(Pai et al., 2006)

some die during P-stage, with Scer\GAL4^e22c

(Pai et al., 2006)

Phenotype Manifest In

anterior crossvein, with Scer\GAL4^Bx-MS1096

(Pai et al., 2006)

dorsal appendage, with Scer\GAL4^55B

(Pai et al., 2006)

dorsal appendage, with Scer\GAL4^EQ1

(Wang and Pai, 2011, Pai et al., 2006)

dorsal appendage, with Scer\GAL4^GR1

(Pai et al., 2006)

egg, with Scer\GAL4^55B

(Pai et al., 2006)

egg, with Scer\GAL4^EQ1

(Pai et al., 2006)

egg, with Scer\GAL4^GR1

(Pai et al., 2006)

egg chorion, with Scer\GAL4^55B

(Pai et al., 2006)

egg chorion, with Scer\GAL4^EQ1

(Pai et al., 2006)

egg chorion, with Scer\GAL4^GR1

(Pai et al., 2006)

eye, with Scer\GAL4^GMR.PF

(Pai et al., 2006)

ommatidium, with Scer\GAL4^GMR.PF

(Pai et al., 2006)

wing vein L4, with Scer\GAL4^Bx-MS1096

(Pai et al., 2006)

wing vein L5, with Scer\GAL4^Bx-MS1096

(Pai et al., 2006)

Detailed Description

Statement

Reference

Somatic clones expressing Cbl^L.UAS under the control of Scer\GAL4^Tub.PU show no obvious differences in the number of intestinal stem cells or the overall number of cells per clone compared to controls.

(Gervais et al., 2019)

The concentration of circulating hemocytes in third instar larvae expressing Cbl^L.Scer\UAS under the control of Scer\GAL4^crq.PO is comparable to that in wild-type controls.

(Kim et al., 2017)

Expression of Cbl^L.Scer\UAS in the follicle cells under the control of Scer\GAL4^EQ1 results in a ventralised eggshell phenotype. Eggs display disrupted dorsal appendage morphology, and fusing or loss of dorsal appendages is observed.

(Wang and Pai, 2011)

Mothers in which Cbl^L.Scer\UAS is driven by Scer\GAL4^55B, Scer\GAL4^GR1 or Scer\GAL4^EQ1 lay eggs with mildly ventralized eggshells: dorsal appendages are reduced and fused. However, the resulting embryos are mostly normal - without obvious signs of ventralisation in their cuticles. The expressivity of eggshell ventralisation induced with Cbl^L.Scer\UAS; Scer\GAL4^hs.PB is most severe (in some cases dorsal appendages are reduced to a thickening of the chorion) if heat shock is applied a oogenesis stage 10.

The wings of Cbl^L.Scer\UAS; Scer\GAL4^Bx-MS1096 adults are small, lack an anterior cross vein and have distally truncated L4 and L5. Cbl^L.Scer\UAS; Scer\GAL4^GMR.PF adults have reduced, rough eyes. Cbl^L.Scer\UAS; Scer\GAL4^e22c animals die during pupal stages.

(Pai et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Cbl^L.UAS, Rbf^120a, Scer\GAL4^{FRT.Rnor\Cd2.Act5C} has increased cell death phenotype, suppressible | partially by mir-998^UAS.cTa, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Truscott et al., 2014)

Enhancer of

Statement

Reference

Cbl^L.UAS/Scer\GAL4^{FRT.Rnor\Cd2.Act5C} is an enhancer of increased cell death phenotype of Rbf^120a

(Truscott et al., 2014)

Suppressor of

Statement

Reference

Cbl^L.UAS, Scer\GAL4^Tub.PU is a suppressor of increased cell number | somatic clone | adult stage phenotype of Scer\GAL4^Tub.PU, kis^HMS01254

(Gervais et al., 2019)

Cbl^L.UAS, Scer\GAL4^crq.PO is a suppressor of increased cell number | third instar larval stage phenotype of Egfr^UAS.cBa, Scer\GAL4^crq.PO

(Kim et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

Cbl^L.UAS, Rbf^120a, Scer\GAL4^{FRT.Rnor\Cd2.Act5C} has eye disc phenotype, suppressible | partially by mir-998^UAS.cTa, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Truscott et al., 2014)

Cbl^L.UAS, Scer\GAL4^EQ1 has dorsal appendage phenotype, suppressible by drk[+]/drk^e0A

(Wang and Pai, 2011)

Cbl^L.UAS, Scer\GAL4^EQ1 has egg phenotype, suppressible | partially by shi^1.UAS

(Pai et al., 2006)

Cbl^L.UAS, Scer\GAL4^EQ1 has egg chorion phenotype, suppressible | partially by shi^1.UAS

(Pai et al., 2006)

Cbl^L.UAS, Scer\GAL4^EQ1 has dorsal appendage phenotype, suppressible | partially by shi^1.UAS

(Pai et al., 2006)

Enhancer of

Statement

Reference

Cbl^L.UAS/Scer\GAL4^{FRT.Rnor\Cd2.Act5C} is an enhancer of eye disc phenotype of Rbf^120a

(Truscott et al., 2014)

Suppressor of

Statement

Reference

Cbl^L.UAS, Scer\GAL4^Tub.PU is a suppressor of adult midgut epithelium | somatic clone phenotype of Scer\GAL4^Tub.PU, kis^HMS01254

(Gervais et al., 2019)

Cbl^L.UAS, Scer\GAL4^Tub.PU is a suppressor of intestinal stem cell | somatic clone | adult stage phenotype of Scer\GAL4^Tub.PU, kis^HMS01254

(Gervais et al., 2019)

Cbl^L.UAS, Scer\GAL4^crq.PO is a suppressor of hemocyte | increased number | third instar larval stage phenotype of Egfr^UAS.cBa, Scer\GAL4^crq.PO

(Kim et al., 2017)

Cbl^L.UAS, Scer\GAL4^EQ1 is a suppressor of dorsal appendage phenotype of Scer\GAL4^EQ1, btl::Egfr^λ.UAS

(Wang and Pai, 2011)

Cbl^L.UAS is a suppressor of dorsal appendage phenotype of Scer\GAL4^EQ1, btl::Egfr^λ.UAS

(Pai et al., 2006)

Cbl^L.UAS is a suppressor of ventral denticle belt | maternal effect phenotype of Scer\GAL4^EQ1, btl::Egfr^λ.UAS

(Pai et al., 2006)

Cbl^L.UAS is a suppressor of wing phenotype of Scer\GAL4^Bx-MS1096, btl::Egfr^λ.UAS

(Pai et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

The increased concentration of circulating hemocytes observed in third instar larvae expressing Egfr^Scer\UAS.cBa under the control of Scer\GAL4^crq.PO is suppressed by co-expression of Cbl^L.Scer\UAS.

(Kim et al., 2017)

The generation of Cbl^L.Scer\UAS mutant eye disc clones enhances the amount of cell death seen in Rbf^120a mutant eye discs. Co-expression of mir-998^Scer\UAS.cTa blocks this suppression.

(Truscott et al., 2014)

drk^e0A/+ significantly suppresses the ventralised egg shell phenotype observed when Cbl^L.Scer\UAS is expressed in follicle cells under the control of Scer\GAL4^EQ1.

Expression of Cbl^L.Scer\UAS suppresses the dorsalised egg shell phenotype observed in animals expressing btl::Egfr^{Scer\UAS.T:λ\cI-DD} under the control of Scer\GAL4^EQ1.

(Wang and Pai, 2011)

The fusion and reduction of dorsal appendages seen in eggs laid by Cbl^L.Scer\UAS; Scer\GAL4^EQ1 mothers is partially suppressed by shi^1.Scer\UAS.

he formation of extra dorsal appendage material in eggs laid by btl::Egfr^{Scer\UAS.T:λ\cI-DD}; Scer\GAL4^EQ1 mothers is suppressed if those mothers also carry Cbl^L.Scer\UAS. The resulting eggs are mildly ventralised. It also largely suppresses the dorsalization of the resulting embryos: extensive denticle belts are formed.

Cbl^L.Scer\UAS suppresses wing defects seen in btl::Egfr^{Scer\UAS.T:λ\cI-DD}; Scer\GAL4^EQ1 adults.

(Pai et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^crq.PO/Cbl^L.UAS rescues Cbl^KG03080

(Kim et al., 2017)

Comments

The increased concentration of circulating hemocytes in Cbl^KG03080 homozygous mutant third instar larvae along with the strongly reduced internalization of sSpi-GFP protein (spi encodes the principal ligand of the EGFR pathway) at high sSpi-GFP conditions (treatment with 30 ng/ml sSpi-GFP) seen in primary hemocytes isolated form the mutant larvae is fully rescued by expression of Cbl^L.Scer\UAS under the control of Scer\GAL4^crq.PO in the Cbl^KG03080 mutants.

(Kim et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Cbl^L.Scer\UAS

Cbl^L.UAS

Name Synonyms

Secondary FlyBase IDs

References (6)

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