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General Information
Symbol
Dmel\His2Av810
Species
D. melanogaster
Name
FlyBase ID
FBal0005491
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
l(3)810, H2Av810, His2AvD810
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Comment:

A 311 bp deletion which removes the second exon of His2Av. Deletion boundaries derived from published sequence.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

311bp deletion, removes second exon.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

His2Av810 third instar larval brain neuroblasts do not exhibit a significant increase in the frequency of chromosomal aberrations, as compared to controls.

His2Av810 homozygotes survive into the late larval stages.

Clonal analyses in imaginal discs show that His2Av810 homozygous cells keep proliferating to form clones.

At two days after clone induction, His2Av810 mutant germline stem cell (GSC) clones are seen in the testis at a similar frequency to controls. By day 8, the percentage of testes with at least one marked GSC clone has dropped to 2%, compared to 64.8% in controls. The number of spermatocytes also falls over time, and by day 12 after clone induction they are no longer observed.

Differentiation of germline cells is unaffected in His2Av810 mutants: as in controls, germline clones homozygous mutant for His2Av810 differentiate into spermatocytes and round and elongating spermatids. Meiosis also occurs as normal and centrosome organisation is unaffected.

At two days after clone induction, homozygous His2Av810 mutant cyst stem cells (CySCs) are seen in the testis at a similar frequency to controls. By day 8, almost all testis lack His2Av810 mutant CySCs, while control CySCs are maintained. His2Av810 mutant cyst cells are lost over time: mutant cyst cells are still seen at 4 days after clone induction but are almost entirely absent by 12 days. Differentiation of cyst cells is unaffected.

Eyes composed entirely of His2Av810 mutant cells (generated using the ey-FLP system) are slightly smaller and rougher compared to controls.

His2Av810mutants exhibit late lethality, with mutant homozygous animals arrested in development in late third-instar larval stage or as prepupae.

His2Av810 mutant exhibit reduced survival after gamma-irradiation.

The position effect variegation at the w locus caused by In(1)wm4h is weakly suppressed by His2Av810/+

His2Av[tCT]; His2Av810 double mutants do not exhibit an increase in levels of X-chromosome nondisjunction, indicating that there are no defects in repairing meiotic DSBs as crossovers in these mutants.

Imaginal discs from larvae expressing His2AvtCT in a His2Av810 background have significantly more radiation-induced apoptotic cells than do discs from control larvae expressing His2Av+t4.0 in a His2Av810 background. This increase in radiation-induced apoptosis does not result in an increase in lethality. In the absence of radiation, both types of imaginal discs have a similar number of apoptotic cells.

Homozygotes die during the third larval instar stage.

Homozygotes undergo a protracted third instar and then die without entering pupation.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

His2Av810 has suppressor of variegation | dominant phenotype, enhanceable by CG8677[+]/CG86773602

Enhancer of
Statement
Reference

His2Av[+]/His2Av810 is an enhancer of visible phenotype of DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU

His2Av[+]/His2Av810 is an enhancer of visible phenotype of Hsap\MAPTGMR.Ex.PJ

His2Av[+]/His2Av810 is an enhancer of suppressor of variegation | dominant phenotype of CG86773602

Suppressor of
Statement
Reference

His2Av[+]/His2Av810 is a suppressor of visible phenotype of E2f1dsRNA.UAS.cJa, Scer\GAL4GMR.PU

His2Av[+]/His2Av810 is a suppressor | partially of visible phenotype of E2f1dsRNA.UAS.cJa, Scer\GAL4ptc-559.1

His2Av810 is a suppressor | partially of visible | dominant phenotype of ash117, trxB11/trx[+]

Other
Phenotype Manifest In
Enhanced by
Statement
Reference
NOT suppressed by
Enhancer of
Statement
Reference

His2Av[+]/His2Av810 is an enhancer of eye phenotype of DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU

His2Av[+]/His2Av810 is an enhancer of eye phenotype of Hsap\MAPTGMR.Ex.PJ

Suppressor of
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

Expression of shgScer\UAS.T:Avic\GFP-rs in germline stem cells (GSCs) under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 does not suppress the GSC loss seen in His2Av810 mutant testes.

His2Av810/Df(3R)BSC524 does not suppress the excess of germline stem cell (GSC)-like cells seen in larval testes when upd1Scer\UAS.cZa is expressed under the control of Scer\GAL4nos.UTR.T:Hsim\VP16, although a few germ cell cysts with branched fusomes are seen.

One copy of domk08108 enhances the loss of His2Av810 mutant germline stem cells in the testis. A greater proportion of testes contain His2Av810 domk08108/+ mutant GSC clones than with His2Av810 alone.

One copy of Iswi2 does not enhance the loss of His2Av810 mutant germline stem cells (GSCs) in the testis. A similar proportion of testes contain His2Av810 mutant GSC clones as with His2Av810 alone.

CG86773602/+ ; His2Av810/+ double heterozygotes show a synergistic effect on the position effect variegation at the w locus caused by In(1)wm4h, showing strong suppression of position effect variegation.

His2Av810/+ enhances the frequency of transformation of the second or third leg to first leg that is seen in Df(3L)Pc/+ flies; 18% of the double heterozygous males have sex combs on the second leg and 72% of the double heterozygous males have sex combs on both the second and third legs (compared to 14.5% of Df(3L)Pc/+ males having sex combs on the second and/or third legs). The frequency of the appearance of an apical bristle on the third leg decreases to 29% in ash117 trxB11 His2Av810 triple heterozygotes compared to ash117 trxB11 double heterozygotes.

Xenogenetic Interactions
Statement
Reference

One copy of His2Av810 enhances the rough eye phenotype seen in flies expressing Hsap\MAPTGMR.Ex.PJ. Necrotic plaques are also seen.

Complementation and Rescue Data
Partially rescued by

His2Av810 is partially rescued by His2AvtM3

His2Av810 is partially rescued by His2AvtM4

His2Av810 is partially rescued by His2AvtM1

His2Av810 is partially rescued by His2AvtM7

His2Av810 is partially rescued by His2AvtM2

Not rescued by
Comments

Expression of His2AvT:Disc\RFP-mRFP rescues the spermatocyte loss seen in His2Av810 testes containing germline stem cell clones. The loss of His2Av810 mutant cyst stem cells (CySCs) is also rescued.

Expression of His2AvScer\UAS.cKa, His2AvS137A.Scer\UAS or His2AvS137E.Scer\UAS under the control of Scer\GAL4arm.PS rescues the viability of His2Av810 flies exposed to 36Gy (gamma irradiation).

Mutant phenotype can be rescued by P element mediated transformation of a wild type copy of His2Av.

Encodes Hist-2AV, a variant form.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 2 )
Crossreferences
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
Synonyms and Secondary IDs (8)
References (29)