FlyBase Allele Report: Dmel\aurA[949]

General Information

Symbol

Dmel\aurA⁹⁴⁹

Species

D. melanogaster

Name

FlyBase ID

FBal0347228

Feature type

allele

Associated gene

Dmel\aurA

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Merigliano et al., 2019)

Mutagen

ethyl methanesulfonate

(Merigliano et al., 2019)

Progenitor genotype

Cytology

Description

Amino acid replacement: W149term.

(Merigliano et al., 2019)

Nucleotide substitution: G477A.

(Merigliano et al., 2019)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:11,963,863..11,963,863 [+]

Nucleotide change:

G11963863A

Reported nucleotide change:

G477A

Amino acid change:

W149term | aurA-PA

Reported amino acid change:

W149term

Comment:

Not clear which G of the TGG of the Trp codon was changed to A to create the stop codon.

(Merigliano et al., 2019)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

centrosome | third instar larval stage

(Merigliano et al., 2019)

chromosome | third instar larval stage

(Merigliano et al., 2019)

chromosome | third instar larval stage (with aurA^87Ac-3)

(Merigliano et al., 2019)

chromosome | third instar larval stage (with Df(3R)Exel6163)

(Merigliano et al., 2019)

chromosome | third instar larval stage | conditional

(Merigliano et al., 2019)

larval brain | third instar larval stage

(Merigliano et al., 2019)

larval brain | third instar larval stage (with aurA^87Ac-3)

(Merigliano et al., 2019)

larval brain | third instar larval stage (with Df(3R)Exel6163)

(Merigliano et al., 2019)

larval brain | third instar larval stage | conditional

(Merigliano et al., 2019)

mitotic spindle | third instar larval stage

(Merigliano et al., 2019)

neuroblast | third instar larval stage

(Merigliano et al., 2019)

neuroblast | third instar larval stage (with aurA^87Ac-3)

(Merigliano et al., 2019)

neuroblast | third instar larval stage (with Df(3R)Exel6163)

(Merigliano et al., 2019)

neuroblast | third instar larval stage | conditional

(Merigliano et al., 2019)

Detailed Description

Statement

Reference

aurA⁹⁴⁹ homozygous third instar larval brain neuroblasts show highly irregular centrosomes and mitotic spindles (e.g. spindle not focused at poles, centrosome present only at one spindle pole and monopolar spindles), higher mitotic index (phospho-H3 staining), lower frequency of anaphases that are more frequently irregular (e.g. chromosome bridges and lagging chromosomes), some chromosomal aberrations (complete and incomplete aberrations), and some aneuploidy/polyploidy; chromosomal aberrations, aneuploidy and polyploidy are almost absent in controls. Similar frequencies of chromosomal aberrations and aneuploidy/polyploidy are also observed in aurA⁹⁴⁹/Df(3R)Exel6163 and aurA⁹⁴⁹/aurA^87Ac-3 transheterozygotes.

Upon X-ray irradiation, aurA⁹⁴⁹ homozygotes show a higher frequency of chromosomal aberrations and slower repair of DNA damage (γ-H2Av foci) than irradiated controls.

(Merigliano et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

aurA⁹⁴⁹ has increased mortality during development phenotype, enhanceable by Brca2^KO/Brca2^56E

(Merigliano et al., 2019)

NOT Enhanced by

Statement

Reference

aurA⁹⁴⁹ has abnormal DNA repair | third instar larval stage | conditional phenotype, non-enhanceable by DNAlig4⁵/DNAlig4⁵

(Merigliano et al., 2019)

NOT suppressed by

Statement

Reference

aurA⁹⁴⁹ has abnormal DNA repair | third instar larval stage | conditional phenotype, non-suppressible by DNAlig4⁵/DNAlig4⁵

(Merigliano et al., 2019)

Phenotype Manifest In

Enhanced by

Statement

Reference

aurA⁹⁴⁹ has larval brain | third instar larval stage phenotype, enhanceable by Brca2^KO/Brca2^56E

(Merigliano et al., 2019)

aurA⁹⁴⁹ has chromosome | third instar larval stage phenotype, enhanceable by rad50^Δ5.1/rad50^Δ5.1

(Merigliano et al., 2019)

aurA⁹⁴⁹ has larval brain | third instar larval stage phenotype, enhanceable by spn-A¹/spn-A¹

(Merigliano et al., 2019)

aurA⁹⁴⁹ has neuroblast | third instar larval stage phenotype, enhanceable by spn-A¹/spn-A¹

(Merigliano et al., 2019)

aurA⁹⁴⁹ has chromosome | third instar larval stage phenotype, enhanceable by spn-A¹/spn-A¹

(Merigliano et al., 2019)

aurA⁹⁴⁹ has neuroblast | third instar larval stage phenotype, enhanceable by Brca2^KO/Brca2^56E

(Merigliano et al., 2019)

aurA⁹⁴⁹ has chromosome | third instar larval stage phenotype, enhanceable by Brca2^KO/Brca2^56E

(Merigliano et al., 2019)

aurA⁹⁴⁹ has larval brain | third instar larval stage phenotype, enhanceable by mei-41^29D/mei-41^29D

(Merigliano et al., 2019)

aurA⁹⁴⁹ has neuroblast | third instar larval stage phenotype, enhanceable by mei-41^29D/mei-41^29D

(Merigliano et al., 2019)

aurA⁹⁴⁹ has chromosome | third instar larval stage phenotype, enhanceable by mei-41^29D/mei-41^29D

(Merigliano et al., 2019)

aurA⁹⁴⁹ has larval brain | third instar larval stage phenotype, enhanceable by rad50^Δ5.1/rad50^Δ5.1

(Merigliano et al., 2019)

aurA⁹⁴⁹ has neuroblast | third instar larval stage phenotype, enhanceable by rad50^Δ5.1/rad50^Δ5.1

(Merigliano et al., 2019)

Suppressed by

Statement

Reference

aurA⁹⁴⁹ has larval brain | third instar larval stage phenotype, suppressible by His2Av⁸¹⁰/His2Av⁸¹⁰

(Merigliano et al., 2019)

aurA⁹⁴⁹ has neuroblast | third instar larval stage phenotype, suppressible by His2Av⁸¹⁰/His2Av⁸¹⁰

(Merigliano et al., 2019)

aurA⁹⁴⁹ has chromosome | third instar larval stage phenotype, suppressible by His2Av⁸¹⁰/His2Av⁸¹⁰

(Merigliano et al., 2019)

aurA⁹⁴⁹ has larval brain | third instar larval stage phenotype, suppressible by tefu^atm-6/tefu^atm-6

(Merigliano et al., 2019)

aurA⁹⁴⁹ has neuroblast | third instar larval stage phenotype, suppressible by tefu^atm-6/tefu^atm-6

(Merigliano et al., 2019)

aurA⁹⁴⁹ has chromosome | third instar larval stage phenotype, suppressible by tefu^atm-6/tefu^atm-6

(Merigliano et al., 2019)

aurA⁹⁴⁹ has larval brain | third instar larval stage phenotype, suppressible | partially by DNAlig4⁵/DNAlig4⁵

(Merigliano et al., 2019)

aurA⁹⁴⁹ has neuroblast | third instar larval stage phenotype, suppressible | partially by DNAlig4⁵/DNAlig4⁵

(Merigliano et al., 2019)

aurA⁹⁴⁹ has chromosome | third instar larval stage phenotype, suppressible | partially by DNAlig4⁵/DNAlig4⁵

(Merigliano et al., 2019)

Enhancer of

Statement

Reference

aurA⁹⁴⁹/aurA⁹⁴⁹ is an enhancer of larval brain | third instar larval stage phenotype of mei-41^29D

(Merigliano et al., 2019)

aurA⁹⁴⁹/aurA⁹⁴⁹ is an enhancer of neuroblast | third instar larval stage phenotype of mei-41^29D

(Merigliano et al., 2019)

aurA⁹⁴⁹/aurA⁹⁴⁹ is an enhancer of chromosome | third instar larval stage phenotype of mei-41^29D

(Merigliano et al., 2019)

aurA⁹⁴⁹/aurA⁹⁴⁹ is an enhancer of larval brain | third instar larval stage phenotype of rad50^Δ5.1

(Merigliano et al., 2019)

aurA⁹⁴⁹/aurA⁹⁴⁹ is an enhancer of neuroblast | third instar larval stage phenotype of rad50^Δ5.1

(Merigliano et al., 2019)

aurA⁹⁴⁹/aurA⁹⁴⁹ is an enhancer of chromosome | third instar larval stage phenotype of rad50^Δ5.1

(Merigliano et al., 2019)

aurA⁹⁴⁹/aurA⁹⁴⁹ is an enhancer of larval brain | third instar larval stage phenotype of Brca2^56E

(Merigliano et al., 2019)

aurA⁹⁴⁹/aurA⁹⁴⁹ is an enhancer of neuroblast | third instar larval stage phenotype of Brca2^56E

(Merigliano et al., 2019)

aurA⁹⁴⁹/aurA⁹⁴⁹ is an enhancer of chromosome | third instar larval stage phenotype of Brca2^56E

(Merigliano et al., 2019)

aurA⁹⁴⁹/aurA⁹⁴⁹ is an enhancer of larval brain | third instar larval stage phenotype of spn-A¹

(Merigliano et al., 2019)

aurA⁹⁴⁹/aurA⁹⁴⁹ is an enhancer of neuroblast | third instar larval stage phenotype of spn-A¹

(Merigliano et al., 2019)

aurA⁹⁴⁹/aurA⁹⁴⁹ is an enhancer of chromosome | third instar larval stage phenotype of spn-A¹

(Merigliano et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

aurA⁹⁴⁹ is rescued by Scer\GAL4^Act.PU/aurA^{UAS.GFP(S65T)}

(Merigliano et al., 2019)

Comments

Expressing aurA^{UAS.GFP(S65T)} under the control of Scer\GAL4^Act.PU rescues the chromosomal aberrations observed in the third instar larval brain neuroblasts of aurA⁹⁴⁹ homozygotes.

(Merigliano et al., 2019)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

aur-A⁹⁴⁹

(Merigliano et al., 2019)

aurA⁹⁴⁹

Name Synonyms

Secondary FlyBase IDs

References (1)

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