Composed of three distinct fragments. First, a 2240 bp His2Av PCR fragment encompassing 751 bp upstream of the His2Av translational initiation codon and the complete His2Av coding region up to the ultimate codon. This is fused to a second PCR fragment encompassing the mRFP1 coding region. A third PCR fragment encompassing 1690 bp of genomic His2Av 3' flanking DNA is cloned downstream of the His2Av-mRFP1 fusion protein.
Expression of His2AvT:Disc\RFP-mRFP rescues the spermatocyte loss seen in His2Av810 testes containing germline stem cell clones. The loss of His2Av810 mutant cyst stem cells (CySCs) is also rescued.
His2AvT:Disc\RFP-mRFP rescues the lethality of His2Av05146/Df(3R)ro80b animals.