FlyBase Allele Report: Dmel\His2Av[mRFP1]

General Information

Symbol

Dmel\His2Av^mRFP1

Species

D. melanogaster

Name

FlyBase ID

FBal0247236

Feature type

allele

Associated gene

Dmel\His2Av

Associated Insertion(s)

Carried in Construct

P{His2Av-mRFP1}

Also Known As

His2Av-mRFP, His2Av-mRFP1, His2Av-RFP, histone-RFP, H2Av-mRFP, H2Av-mRFP1, His-RFP, His2AvD-mRFP, H2AvD-mRFP, H2A-RFP

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

in vitro construct - coding region fusion

(Schuh et al., 2007, Heeger et al., 2005)

in vitro construct - genomic fragment

(Schuh et al., 2007)

in vitro construct

(Wang et al., 2011)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{His2Av-mRFP1}

Cytology

Nature of the lesion

Statement

Reference

Composed of three distinct fragments. First, a 2240 bp His2Av PCR fragment encompassing 751 bp upstream of the His2Av translational initiation codon and the complete His2Av coding region up to the ultimate codon. This is fused to a second PCR fragment encompassing the mRFP1 coding region. A third PCR fragment encompassing 1690 bp of genomic His2Av 3' flanking DNA is cloned downstream of the His2Av-mRFP1 fusion protein.

(Schuh et al., 2007)

Allele components

Product class / Tool use(s)

Regulatory region(s)

His2Av

Encoded product / tool

His2Av

Tagged with

mRFP1

red fluorescent protein

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

His2Av^mRFP1 rescues His2Av⁸¹⁰

(Morillo Prado et al., 2013)

His2Av^mRFP1/His2Av^{T:Disc\RFP-mRFP} rescues His2Av⁰⁵¹⁴⁶/Df(3R)ro80b

(Schuh et al., 2007)

Comments

Expression of His2Av^{T:Disc\RFP-mRFP} rescues the spermatocyte loss seen in His2Av⁸¹⁰ testes containing germline stem cell clones. The loss of His2Av⁸¹⁰ mutant cyst stem cells (CySCs) is also rescued.

(Morillo Prado et al., 2013)

His2Av^{T:Disc\RFP-mRFP} rescues the lethality of His2Av⁰⁵¹⁴⁶/Df(3R)ro80b animals.

(Schuh et al., 2007)

Images (0)

Mutant

Wild-type

Stocks (9)

Bloomington

23651

w^*; P{His2Av-mRFP1}II.2

23650

w^*; P{His2Av-mRFP1}III.1