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Reference
Citation
Heeger, S., Leismann, O., Schittenhelm, R., Schraidt, O., Heidmann, S., Lehner, C.F. (2005). Genetic interactions of separase regulatory subunits reveal the diverged Drosophila Cenp-C homolog.  Genes Dev. 19(17): 2041--2053.
FlyBase ID
FBrf0187592
Publication Type
Research paper
Abstract

Faithful transmission of genetic information during mitotic divisions depends on bipolar attachment of sister kinetochores to the mitotic spindle and on complete resolution of sister-chromatid cohesion immediately before the metaphase-to-anaphase transition. Separase is thought to be responsible for sister-chromatid separation, but its regulation is not completely understood. Therefore, we have screened for genetic loci that modify the aberrant phenotypes caused by overexpression of the regulatory separase complex subunits Pimples/securin and Three rows in Drosophila. An interacting gene was found to encode a constitutive centromere protein. Characterization of its centromere localization domain revealed the presence of a diverged CENPC motif. While direct evidence for an involvement of this Drosophila Cenp-C homolog in separase activation at centromeres could not be obtained, in vivo imaging clearly demonstrated that it is required for normal attachment of kinetochores to the spindle.

PubMed ID
PubMed Central ID
PMC1199574 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Genes Dev.
    Title
    Genes & Development
    Publication Year
    1987-
    ISBN/ISSN
    0890-9369
    Data From Reference
    Aberrations (11)
    Alleles (49)
    Genes (19)
    Cell Lines (1)
    Natural transposons (1)
    Insertions (2)
    Experimental Tools (5)
    Transgenic Constructs (28)