CENP-A, CenH3, CID/CENP-A, dCENP-A, centromere protein A
Gene model reviewed during 5.49
There is only one protein coding transcript and one polypeptide associated with this gene
Forms a nucleosome-like histone octamer containing two molecules each of H2A, H2B, cid and H4 assembled in one cid-H4 heterotetramer and two H2A-H2B heterodimers (By similarity). The cid-H4 heterotetramer is more compact and structurally more rigid than corresponding H3-H4 heterotetramers (By similarity). Interacts with the condensin subunit Cap-G (PubMed:15592865). Interacts with Chrac-14 (PubMed:24703848).
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\cid using the Feature Mapper tool.
Protein can be detected as 4 distinct foci of staining at the centromere region of chromatids of developing spermatozoa. While staining cannot be detected in very late spermatids and mature spermatozoa, the highly compact nature of the sperm nuclei have been shown to exclude antibodies and therefore prevent visualization of proteins associated with these structures. Shortly after fertilization protein can be detected again as discrete spots on the apposed pronuclei.
GBrowse - Visual display of RNA-Seq signalsView Dmel\cid in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
RNAi screen using dsRNA made from templates generated with primers directed against this gene results in chromosome misalignment on the metaphase spindle and the formation of aberrantly long spindles when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.
New incorporation of cid protein into centromeres takes place during anaphase of the syncytial divisions of embryos. This incorporation is independent of DNA replication and of normal pulling forces generated by the mitotic spindle, but is strictly coupled to mitotic progression.
SL2 cells treated with dsRNA against cid show mitotic arrest and chromosomes are displaced from the metaphase plate.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.