FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Hat1, cid
FlyBase ID
FBig0000104287
Interaction Network
Interactions Browser links
Hat1 network
cid network
Reported Interactions
FBrf0231314-1.coIP.WB
Reference
Boltengagen et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Hat1 

HAT1

bait

FLAG tag

cid 

CENP-A

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of baculovirus-infected Sf9 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Boltengagen et al., 2016)
FBrf0231314-14.coIP.MS
Reference
Boltengagen et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
cid 

CENP-A

bait

GFP tag

Hat1 

CG2051

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Boltengagen et al., 2016)
FBrf0231314-16.coIP.MS
Reference
Boltengagen et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Hat1 

CG2051

bait

Strep tag, FLAG tag

cid 

CENP-A

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Boltengagen et al., 2016)
FBrf0231314-21.coIP.WB
Reference
Boltengagen et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Hat1 

CG2051

bait

FLAG tag

cid 

CENP-A

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

CG2051 interacts with Caf1-55, cid and H4K5Ac-modified His4 in this fraction, but not cal1, dre4 or Ssrp.

(Boltengagen et al., 2016)

Source was S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Boltengagen et al., 2016)

A gel filtration fraction ("B15") containing complexes of about 180 kDa was used for immunoprecipitation.

(Boltengagen et al., 2016)
FBrf0231314-23.coIP.WB
Reference
Boltengagen et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
cid 

CENP-A

bait
Hat1 

CG2051/HAT

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was chromatin extract of S2 cells; bait produced from endogenous gene; prey produced from transfected construct; prey produced from transfected construct.

(Boltengagen et al., 2016)
FBrf0231314-25.coIP.WB
Reference
Boltengagen et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Hat1 

HAT1

bait

FLAG tag

cid 

CENP-A

prey

V5 tag

cid 
cid 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cid 
C-terminal region
sufficient binding region
aa 69-225
cid 
CG2051 binding region
necessary binding region
aa 69-125
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of baculovirus-infected Sf9 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Boltengagen et al., 2016)
FBrf0231314-26.coIP.WB
Reference
Boltengagen et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cid 

CENP-A

bait

V5 tag

Hat1 

HAT1

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of baculovirus-infected Sf9 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Boltengagen et al., 2016)
FBrf0231314-9.coIP.MS
Reference
Boltengagen et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
cid 

CENP-A

bait

Strep tag, FLAG tag

Hat1 

CG2051

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Boltengagen et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)