FB2026_02 , released June 18, 2026
Cell Line: S2-ThermoFischer
Open Close
General Information
Symbol
S2-ThermoFischer
Species
D. melanogaster
Feature type
cultured cell-line
FlyBase ID
FBtc9000008
Source
Tissue Source
embryo
(FlyBase Curators, 2016)
Developmental Stage
late embryonic stage
(FlyBase Curators, 2016)
Lab of Origin

Invitrogen

(FlyBase Curators, 2016)
Characterization
Sex
Karyotype
Associated Genes (0)
    Integrated Constructs (0)
      Comments

      New stable cell line derived from this cell line: A CRISPR method for S2 cells that can be adapted for both knock-in and knock-out of any gene was developed. The followind lines were created (author designations): S2-Cas9Par, S2-Plk4 CRISPR null line 1, S2-Plk4 CRISPR null line 2.2, S2-Plk4 CRISPR null line 7, S2-Ana2 CRISPR null line 2, S2-Ana2 CRISPR null line 4, S2-Ana2 CRISPR null line 5, S2-ana2 CRISPR null line 12, Ana2 CRISPR null line 15, and Ana2 CRISPR null line 17. Note that Plk4 is SAK in FlyBase and Ana2 is ana2 in FlyBase.

      (Ryniawec et al., 2024)

      New stable cell line derived from this cell line: Stable S2 cell lines were created expressing Tag:FLAG-tagged baf, GFP-baf, RFP-Lam or GFP-Lam.

      (Li et al., 2024)

      New stable cell line derived from this cell line: The following stable S2 cell lines were created: S2-OsTir1-CG11247/Saft-AID, S2-OsTir1-Su(Hw)-AID, S2-OsTir1-Phaser-AID, S2-OsTir1-G9a-AID, S2-OsTir1-Lft-deltaDLM3, S2-TurboID-GFP, S2-CG11247/Saft-TurboID.

      (Hofbauer et al., 2024)

      New stable cell line derived from this cell line: The following derivative cell lines were generated: Parental OsTir-expressing S2 cell line, Trf2 C-terminally-tagged AID S2 cell line, Tbp-N-terminally-tagged AID S2 cell line, Dref-N-terminally-tagged AID S2 cell line, TfIIA-L-C-terminally-tagged AID S2 cell line, Chro-N-terminally-tagged AID S2 cell line.

      (Serebreni et al., 2023)

      New stable cell line derived from this cell line: A collection of stable Drosophila S2 cell lines was established, each one expressing a (His)6-PC-SNAP-tagged version of each Elongator complex subunit, Elp1, Elp2, and Elp3.

      (Planelles-Herrero et al., 2022)

      New stable cell line derived from this cell line: Stable S2 cell lines were created to study chromatin remodeling. The base S2[OsTir1] was created and selected core proteins from the four main sub-families of ATP-dependent chromatin remodelers (Snr1, Iswi, Ino80, and Chd1) as well as SWI/SNF component Brd7-9 were inserted to create S2OsTir1-Snr1-AID, S2OsTir1-Iswi-AID, S2OsTir1-Ino80-AID, S2OsTir1-Chd1-AID, and S2OsTir1-Brd7-AID for component depletion studies.

      (Hendy et al., 2022)

      New stable cell line derived from this cell line: Centriolar proteins were tagged with GFP at either the N- or C- terminus and used to create stable S2 cell lines. S2 cell lines constitutively expressing the following constructs were created: asl-GFP, Sas-6-GFP, ana1-GFP, GFP-ana1, Cep135-GFP, ana3-GFP, Rcd4-GFP, ana2-GFP, GFP-asl.

      (Tian et al., 2021)

      New stable cell line derived from this cell line: S2 cell lines expressing full-length or fragments of dachs tagged with Tag:V5 under the control of the MT promoter were created.

      (Nagai et al., 2021)

      New stable cell line derived from this cell line: S2 cells expressing V5-tagged fragments of dachs were generated. The fragments used were D-F (full-length dachs), D-N (N-terminal fragment of dachs), D-M (myosin domain of dachs), and D-C (C-terminal fragment of dachs).

      (Nagai et al., 2021)

      New stable cell line derived from this cell line: S2 cells harboring stably integrated Tag:HA-ZsGreen tethering reporters were created.

      (Eastwood et al., 2021)

      New stable cell line derived from this cell line: A stable S2- mCherry:Tubulin-GFP:cid cell line was created. This was accomplished by transfecting cells stably expressing GFP:CID (from Gary Karpen) with inducible mCherry-alpha-tubulin.

      (Dewey et al., 2020)

      New stable cell line derived from this cell line: Stable cell lines established expressing GFP-tagged ana3 or Rcd4 under the control of the Act5C promoter.

      (Panda et al., 2020)

      New stable cell line derived from this cell line: A stable S2 cell line expressing GFP-Lam and mCherry-alpha-tubulin was created. Stable S2 lines expressing RFP(Unk)-Lam wild type and constructs in which the CDK sites were mutated were generated. A stable S2 cell line that expressed GFP-baf and mCherry-alpha-tubulin was created.

      (Mehsen et al., 2018)

      New stable cell line derived from this cell line: Stable cell lines were constructed for expression of brm constructs (recBRM-V5 and recBRM-K804R-V5).

      (Jordán-Pla et al., 2018)

      New stable cell line derived from this cell line: stable S2 cell lines expressing Tag:TAP-tagged forms of Bet3 and the Drosophila orthologs of the larger TRAPPC subunits (brun, SIDL, gry, CG11396, and CG4953) were generated as well as a Tag:TAP-tagged form of CG1116.

      (Riedel et al., 2018)

      New stable cell line derived from this cell line: S2 cells stably expressing Ser tagged with tdTomato] were used.

      (Lee et al., 2017)

      New stable cell line derived from this cell line: S2 cells stably expressing Tag:FLAG-jumu were created.

      (Hao and Jin, 2017)

      S2 cells obtained from Invitrogen. Many features of the S2 cell line suggest that it is derived from a macrophage-like lineage.

      (FlyBase Curators, 2016)

      New stable cell line derived from this cell line: S2 cell lines stably expressing Rren\LUC were created.

      (Wakiyama et al., 2011)

      New stable cell line derived from this cell line: Met-GAL4-expressing S2 cells were utilized.

      (Besse et al., 2007)

      New stable cell line derived from this cell line: Stable S2 cell lines expressing AGO1 containing Tag:FLAG followed by Tag:HA were generated.

      (Easow et al., 2007)

      New stable cell line derived from this cell line: Stable S2 cell lines expressing Tag:polyHis-tagged-PGRP-LF were generated.

      (Persson et al., 2007)

      New stable cell line derived from this cell line: Stable S2 cell lines overexpressing an N-terminal fragment of hh were generated.

      (Molnar et al., 2007)

      New stable cell line derived from this cell line: Stable S2 cell lines that contain an inducible (MT) Myc transgene or constitutively expressed GFP under the control of the Act5C promoter were generated.

      (Senoo-Matsuda and Johnston, 2007)

      New stable cell line derived from this cell line: Stable S2 cell lines that express Tag:FLAG-esc proteins, including full-length, N-terminally truncated (residues 61 to 425), and C-terminally truncated (residues 1 to 169) forms, as well as a Tag:FLAG-Pc line, were generated.

      (Tie et al., 2007)

      New stable cell line derived from this cell line: Stable S2 cell lines expressing Human ADAM33 under the control of the Act5C or metallothionein promoter were generated.

      (Prosise et al., 2004)
      Parental Lines (1)
      Isolate of:
      Descendant Lines (1)
       
      Transformant of S2-ThermoFischer:
      External Stocks and Resources ( 3 )
      Crossreferences
      Cellosaurus - Cellosaurus is a knowledge resource on cell lines that attempts to describe all cell lines used in biomedical research.
      Invitrogen -
      RRID - Research Resource Identification Portal
      Synonyms and Secondary IDs (29)
      Reported As
      Symbol Synonym
      CVCL_Z232
      (Bairoch, 2016)
      D-Mel
      (Emond-Fraser et al., 2023, Archambault et al., 2008)
      D-Mel2
      (Kovacs et al., 2018, Mehsen et al., 2018)
      D.Mel
      (Ito et al., 2019)
      D.Mel-2
      (Shaukat et al., 2024, Fatalska et al., 2021, Karman et al., 2020, Panda et al., 2020, Umer et al., 2019, Riedel et al., 2018, Sorge et al., 2012, Zhu et al., 2007, Hofmann and Munro, 2006)
      D.Mel2
      (Borovsky et al., 2022, Tian et al., 2021)
      D.mel-2
      (Li et al., 2025, Meghini et al., 2023, Arvola et al., 2020)
      D.mel2
      (Lie-Jensen et al., 2019)
      DMel-2
      (Behm-Ansmant et al., 2007)
      Dmel-2
      (Heigwer et al., 2023, Haugen et al., 2022, Smith et al., 2008, Wagner et al., 2007, Bilioni et al., 2005, Francis et al., 2002)
      Dmel2
      (Tian et al., 2021, Hirst et al., 2009, Pan and Hardie, 2002, Adams et al., 2001)
      Drosophila S2 Cells
      Drosophila S2 Cells in Schneider's Medium
      FBtc9000008
      (Lie-Jensen et al., 2019)
      R69007
      (Zhang et al., 2021)
      RRID:CVCL_Z232
      (Eastwood et al., 2021, Bairoch, 2016)
      S2
      (Gao et al., 2025, Li et al., 2025, Pachinger et al., 2025, Sun et al., 2025, Sun et al., 2025, Benchorin et al., 2024, Cai et al., 2024, Hermant et al., 2024, Hersperger et al., 2024, Hofbauer et al., 2024, Klein et al., 2024, Li et al., 2024, Loubiere et al., 2024, Makhijani et al., 2024, Parra and Johnston, 2024, Ryniawec et al., 2024, Shaukat et al., 2024, Shimizu et al., 2024, Singh et al., 2024, Stephan et al., 2024, Sung et al., 2024, Zhou et al., 2024, Barrington et al., 2023, Bunker et al., 2023, Chai et al., 2023, Eleazer et al., 2023, Fan et al., 2023, Galletta et al., 2023, Heigwer et al., 2023, Hidalgo et al., 2023, Klaus et al., 2023, Ryniawec et al., 2023, Scholz et al., 2023, Serebreni et al., 2023, Tabuloc et al., 2023, Terzioglu et al., 2023, van den Berg et al., 2023, Zhang et al., 2023, Baccino-Calace et al., 2022, Chen et al., 2022, Hendy et al., 2022, Heymann et al., 2022, Khyzha et al., 2022, Kietz et al., 2022, Kovacs et al., 2022, Llobet Rosell et al., 2022, Mathieu et al., 2022, Planelles-Herrero et al., 2022, Sadasivan et al., 2022, Starczak et al., 2022, Tsapras et al., 2022, Xu et al., 2022, Cai et al., 2021, Keegan et al., 2021, Liu et al., 2021, Mak et al., 2021, Munafò et al., 2021, Nagai et al., 2021, On et al., 2021, Rao et al., 2021, Shi et al., 2021, Tian et al., 2021, Valsecchi et al., 2021, Zhang et al., 2021, Zhou et al., 2021, Alecki et al., 2020, Dewey et al., 2020, Dobbelaere et al., 2020, He et al., 2020, Huang et al., 2020, Johnston, 2020, Kwon et al., 2020, Li et al., 2020, Maddison et al., 2020, Mendes et al., 2020, Oh et al., 2020, Singh et al., 2020, Wurster et al., 2020, Aalto et al., 2019, Bronkhorst et al., 2019, Cabral et al., 2019, Chang et al., 2019, Fabry et al., 2019, Gambarotto et al., 2019, González de Cózar et al., 2019, Han et al., 2019, Kneuss et al., 2019, Kono et al., 2019, Lie-Jensen et al., 2019, Matveeva et al., 2019, Munafò et al., 2019, Palmer et al., 2019, Pandey et al., 2019, Sato et al., 2019, Swider et al., 2019, Tsarouhas et al., 2019, Xie et al., 2019, Boese et al., 2018, Gerards et al., 2018, Goto et al., 2018, Hao et al., 2018, Heigwer et al., 2018, Issa et al., 2018, Jordán-Pla et al., 2018, Kerr et al., 2018, Lam et al., 2018, Lee et al., 2018, Lee et al., 2018, Liang et al., 2018, Mashtalir et al., 2018, Nayak et al., 2018, Pan et al., 2018, Rey et al., 2018, Riedel et al., 2018, Rosa-Ferreira et al., 2018, Schürmann et al., 2018, Takáts et al., 2018, Yang and Edery, 2018, Zhang et al., 2018, Dewey and Johnston, 2017, Dzhindzhev et al., 2017, Hao and Jin, 2017, Kandul et al., 2017, Lee et al., 2017, Lee et al., 2017, Liu et al., 2017, Maki et al., 2017, Rissland et al., 2017, Salvia et al., 2017, Solis et al., 2017, Wang et al., 2017, Chiu, 2016.5.31, Delatte et al., 2016, Faden et al., 2016, FlyBase Curators, 2016, Kandul et al., 2016, Karlikow et al., 2016, Tao et al., 2016, Veeranan-Karmegam et al., 2016, Antonello et al., 2015, Chittaranjan et al., 2015, Dewey et al., 2015, He et al., 2015, Kerr et al., 2015, Kwok et al., 2015, Lee et al., 2015, Park et al., 2015, Vallejo et al., 2015, Zhang et al., 2015, DeVorkin et al., 2014, Fukuoh et al., 2014, Kim et al., 2014, Becnel et al., 2013, Jukam et al., 2013, Follmer et al., 2012, Gontijo et al., 2011, Qian et al., 2011, Saez et al., 2011, Venkei et al., 2011, Vodovar et al., 2011, Wakiyama et al., 2011, Kametaka et al., 2010, Montembault et al., 2010, Morozova et al., 2010, Blanchette et al., 2009, Knowles, 2009, Lauwers et al., 2009, Shen et al., 2009, Sopko et al., 2009, Yang et al., 2009, Chalkley et al., 2008, Freel et al., 2008, Kim et al., 2008, Lee et al., 2008, Reed et al., 2008, Smart et al., 2008, Besse et al., 2007, Easow et al., 2007, Kim et al., 2007, Molnar et al., 2007, Persson et al., 2007, Senoo-Matsuda and Johnston, 2007, Tie et al., 2007, Tsichritzis et al., 2007, Xie et al., 2007, Bhaskara et al., 2006, D'Avino et al., 2006, Orme et al., 2006, Ryabinina et al., 2006, Saleh et al., 2006, Voolstra et al., 2006, Muro et al., 2004, Prosise et al., 2004, Zeidler et al., 2004)
      S2 (Invitrogen-R69007)
      (Han et al., 2022)
      S2 (Thermofisher)
      (Hardy et al., 2023)
      S2 Thermo Fisher
      (Plutoni et al., 2019)
      S2 cells (Invitrogen)
      (Khong et al., 2017)
      S2 cells from GIBCO
      (Li et al., 2020)
      S2 from ThermoFisher
      (Molodtsov et al., 2016)
      S2-Invitrogen
      S2-Thermo Fisher
      (Eastwood et al., 2021)
      S2-ThermoFischer
      Schneider
      (Antonello et al., 2015)
      Schneider 2
      (Planelles-Herrero et al., 2022, Xu et al., 2022, Schürmann et al., 2018, Bairoch, 2016)
      d.mel-2
      (Connacher et al., 2024, Li et al., 2024, Emond-Fraser et al., 2023)
      Name Synonyms
      Secondary FlyBase IDs
        References (268)