Sas6, DSas-6, Dsas6
cytoskeleton - centriole assembly - cartwheel component have an underappreciated role in basal body
Please see the JBrowse view of Dmel\Sas-6 for information on other features
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AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Gene model reviewed during 5.47
There is only one protein coding transcript and one polypeptide associated with this gene
Nine homodimers form a cartwheel structure with an internal diameter of 23 nM and radial spokes connecting to the microtubule triplets (By similarity). Homodimer (PubMed:33704067). Interacts with Gorab (via C-terminus); binds as a homodimer to a Gorab monomer (PubMed:29892014, PubMed:33704067).
The 35 nM long coiled-coil domain mediates homodimerization while the globular N-terminus links the dimers at an angle of 40 degrees to form the inner ring.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Sas-6 using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
JBrowse - Visual display of RNA-Seq signals
View Dmel\Sas-6 in JBrowse3-100
3-97.4
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
New stable cell line derived from S2-ThermoFischer : Centriolar proteins were tagged with GFP at either the N- or C- terminus and used to create stable S2 cell lines. S2 cell lines constitutively expressing the following constructs were created: asl-GFP, Sas-6-GFP, ana1-GFP, GFP-ana1, Cep135-GFP, ana3-GFP, Rcd4-GFP, ana2-GFP, GFP-asl.
sas-6 is involved in centriole duplication.
RNAi screen using dsRNA made from templates generated with primers directed against this gene results in the spindles that are either anastral (no γ-tubulin staining at the poles) or monastral (only one pole with γ-tubulin staining) when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.
Source for identity of: Sas-6 CG15524
Named "Sas-6" after the C. elegans ortholog.
