FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
ana1, Sas-6
FlyBase ID
FBig0000105296
Interaction Network
Interactions Browser links
ana1 network
Sas-6 network
Reported Interactions
FBrf0233282-142.coIP.WB
Reference
Galletta et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Sas-6 

Sas-6

bait

GFP tag

ana1 

Ana1

prey

FLAG tag

ana1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ana1 
region
sufficient binding region
aa 1311-1729

construct "F4", coordinates relative to ana1-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Galletta et al., 2016)
FBrf0233282-24.Y2H
Reference
Galletta et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ana1 

Ana1

bait

fused to GAL4 DNA-binding domain

ana1 
Sas-6 

Sas-6

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ana1 
C-terminal region
sufficient binding region
aa 1311-1729

coordinates relative to ana1-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Galletta et al., 2016)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Galletta et al., 2016)

Weak interaction.

(Galletta et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)