FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Gene: Dmel\ana3
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General Information
Symbol
Dmel\ana3
Species
D. melanogaster
Name
anastral spindle 3
Annotation Symbol
CG13162
Feature Type
protein_coding_gene
FlyBase ID
FBgn0266111
Gene Model Status
Current
Stock Availability
2 publicly available
Gene Summary
anastral spindle 3 (ana3) encodes a centriole and basal body localized protein that is required for the proper function of these organelles. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)
All Summaries
Key Links
Genomic Location
Cytogenetic map
48F11-49A1
Sequence location
Recombination map
2-67
RefSeq locus
NT_033778 REGION:12344983..12351325
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (11 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables protein binding
inferred from physical interaction with UniProtKB:Q9VLM6
(Panda et al., 2020)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (6 terms)
Terms Based on Experimental Evidence (5 terms)
CV Term
Evidence
References
involved_in centriole assembly
inferred from mutant phenotype
(Panda et al., 2020)
involved_in centriole replication
inferred from mutant phenotype
(Dobbelaere et al., 2008)
involved_in centriole-centriole cohesion
inferred from mutant phenotype
(Stevens et al., 2009)
involved_in centrosome cycle
inferred from mutant phenotype
(Stevens et al., 2009)
involved_in ciliary basal body organization
inferred from mutant phenotype
(Stevens et al., 2009)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
involved_in centriole replication
inferred from biological aspect of ancestor with PANTHER:PTN001271356
(GO Reference Genome Project, 2011-)
involved_in centriole-centriole cohesion
inferred from biological aspect of ancestor with PANTHER:PTN001271356
(GO Reference Genome Project, 2011-)
involved_in ciliary basal body organization
inferred from biological aspect of ancestor with PANTHER:PTN001271356
(GO Reference Genome Project, 2011-)
involved_in cilium organization
inferred from electronic annotation with InterPro:IPR030791
(InterPro Project Members, 2004-)
Cellular Component (4 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
located_in centriole
inferred from direct assay
(Panda et al., 2020)
inferred from direct assay
(Stevens et al., 2009)
located_in ciliary basal body
inferred from direct assay
(Stevens et al., 2009)
located_in ring centriole
inferred from direct assay
(Panda et al., 2020)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
is_active_in centriole
inferred from biological aspect of ancestor with PANTHER:PTN001271356
(GO Reference Genome Project, 2011-)
located_in centrosome
inferred from electronic annotation with InterPro:IPR030791
(InterPro Project Members, 2004-)
is_active_in ciliary basal body
inferred from biological aspect of ancestor with PANTHER:PTN001271356
(GO Reference Genome Project, 2011-)
located_in ciliary basal body
inferred from electronic annotation with InterPro:IPR030791
(InterPro Project Members, 2004-)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the rotatin family. (A1Z8X3)
Protein Signatures (InterPro)
Summaries
Gene Snapshot
anastral spindle 3 (ana3) encodes a centriole and basal body localized protein that is required for the proper function of these organelles. [Date last reviewed: 2019-03-07]
Protein Function (UniProtKB)
Participes in the structural integrity of both centrioles and basal bodies and in centriole cohesion (PubMed:19948479). Participates in the later stages of centriole assembly through the interaction with Rcd4 leading to the centriole to centrosome conversion (PubMed:32543652).
(UniProt, A1Z8X3)
Summary (Interactive Fly)

a member of the pericentriolar matrix proteins, a key component of centriolar cohesion and basal body formation - actively promotes cell survival - stabilize Sol naraes for cell survival and proliferation - responds to irradiation at the front line - During centriole biogenesis, Ana3 and Rcd4 are sequentially loaded on the newly formed centriole and are required for centriole-to-centrosome conversion through recruiting the Cep135-Ana1-Asterless complex

Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\ana3 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry A1Z8X3)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.49

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
ana3-RA
FBtr0087904
NM_136921
6284
1977
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
ana3-PA
FBpp0087015
225.6
1977
7.90
A1Z8X3
NP_610765
AAF58528
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

Interacts with Rcd4;this complex is recruited to daughter centrioles before their conversion to centrosomes.

(UniProt, A1Z8X3)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
UniProt
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ana3 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.22

Transcript Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
day 5 of adulthood | male -- day 7 of adulthood | male
spermatozoon
(Wasbrough et al., 2010)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in centriole
inferred from direct assay
(Panda et al., 2020)
inferred from direct assay
(Stevens et al., 2009)
located_in ciliary basal body
inferred from direct assay
(Stevens et al., 2009)
located_in ring centriole
inferred from direct assay
(Panda et al., 2020)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\ana3 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 5 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of ana3
Transgenic constructs containing regulatory region of ana3
Aberrations (Deficiencies and Duplications) ( 7 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (1)
Hsap\RTTN
5 of 14
Yes
Yes
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (1)
Rnor\Rttn
2 of 14
Yes
Yes
Mus musculus (laboratory mouse) (1)
Mmus\Rttn
3 of 14
Yes
Yes
Xenopus tropicalis (Western clawed frog) (1)
Xtro\rttn
2 of 13
Yes
Yes
Danio rerio (Zebrafish) (1)
Drer\rttn
4 of 14
Yes
Yes
Caenorhabditis elegans (Nematode, roundworm) (0)
Anopheles gambiae (African malaria mosquito) (1)
Agam\AgaP_AGAP011454
11 of 12
Yes
Yes
Arabidopsis thaliana (thale-cress) (0)
Saccharomyces cerevisiae (Brewer's yeast) (0)
Schizosaccharomyces pombe (Fission yeast) (0)
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:ana3. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 1 )
    Allele
    Disease
    Evidence
    References
    ana3unspecified
    model of  dilated cardiomyopathy
    CEA
    (Chun et al., 2023)
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    RTTN; rotatin
    5 of 14
    610436
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Interactions
      Summary of Physical Interactions
      Summary of Genetic Interactions
      Interaction Browsers
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      External Data
      Subunit Structure (UniProtKB)
      Interacts with Rcd4;this complex is recruited to daughter centrioles before their conversion to centrosomes.
      (UniProt, A1Z8X3 )
      Linkouts
      DroID - A comprehensive database of gene and protein interactions.
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Pathways
      Signaling Pathways (FlyBase)
      Metabolic Pathways
      FlyBase
      External Links
      External Data
      Linkouts
      Class of Gene
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      2R
      Recombination map
      2-67
      Cytogenetic map
      48F11-49A1
      Sequence location
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      48F11-49A1
      Left limit from in situ hybridisation (FBrf0157097) Right limit from in situ hybridisation (FBrf0157097)
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      48F11-49A1
      (Oh et al., 2003)
      Experimentally Determined Recombination Data
      Location

      2-67

      (FlyBase, 2016)

      2-68.3

      (Comeron et al., 2012)
      Left of (cM)
      Right of (cM)
      Notes
      Stocks and Reagents
      Stocks (2)
      Genomic Clones (16)
       

      Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

      cDNA Clones (0)
       

      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

      cDNA clones, fully sequenced
      BDGP DGC clones
        Other clones
          Drosophila Genomics Resource Center cDNA clones

          For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

            cDNA Clones, End Sequenced (ESTs)
            BDGP DGC clones
              Other clones
                RNAi and Array Information
                Linkouts
                Antibody Information
                Laboratory Generated Antibodies
                 

                polyclonal

                (Panda et al., 2020, Stevens et al., 2009)
                Commercially Available Antibodies
                 
                Cell Line Information
                Publicly Available Cell Lines
                 
                  Other Stable Cell Lines
                   
                  Other Comments

                  ana3 is involved in centriole duplication.

                  (Dobbelaere et al., 2008)

                  RNAi screen using dsRNA made from templates generated with primers directed against this gene results in the spindles that are either anastral (no γ-tubulin staining at the poles) or monastral (only one pole with γ-tubulin staining) when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

                  (Goshima et al., 2007)

                  1 allele of l(2)SH0558 recovered in a P-insertion screen.

                  (Oh et al., 2003)
                  Relationship to Other Genes
                  Source for database merge of

                  Source for merge of: ana3 l(2)SH0558

                  (Stevens et al., 2009)
                  Additional comments
                  Nomenclature History
                  Source for database identify of
                  Nomenclature comments
                  Etymology

                  The name "anastral spindle 3" refers to the cellular phenotype observed following treatment of S2 cells with dsRNA directed against this gene.

                  (Goshima et al., 2007)
                  Synonyms and Secondary IDs (7)
                  Datasets (0)
                  Study focus (0)
                  Experimental Role
                  Project
                  Project Type
                  Title
                  Study result (0)
                  Result
                  Result Type
                  Title
                  External Crossreferences and Linkouts ( 25 )
                  Sequence Crossreferences
                  NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                  GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                  GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                  RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                  UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                  UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
                  Other crossreferences
                  AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                  DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                  EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                  FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                  InterPro - A database of protein families, domains and functional sites
                  KEGG Genes - Molecular building blocks of life in the genomic space.
                  MARRVEL_MODEL - MARRVEL (model organism gene)
                  Linkouts
                  DroID - A comprehensive database of gene and protein interactions.
                  Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                  FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                  FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                  iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                  Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
                  MIST (protein-protein) - An integrated Molecular Interaction Database
                  References (43)