FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
ana3, Rcd4
FlyBase ID
FBig0000120487
Interaction Network
Interactions Browser links
ana3 network
Rcd4 network
Reported Interactions
FBrf0245965-1.coIP.MS
Reference
Panda et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-ThermoFischer
Participants
Corresponds to
Reported as
Role
Note
Rcd4 

Rcd4

bait

GFP tag

ana3 

Ana3

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Panda et al., 2020)
FBrf0245965-2.coIP.MS
Reference
Panda et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-ThermoFischer
Participants
Corresponds to
Reported as
Role
Note
ana3 

Ana3

bait

GFP tag

Rcd4 

Rcd4

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Panda et al., 2020)
FBrf0245965-3.PD.WB
Reference
Panda et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rcd4 

Rcd4

bait

GST tag

ana3 

Ana3

prey

MBP tag

ana3 
ana3 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ana3 
C-terminal domain
sufficient binding region
aa 1195-1977

coordinates relative to ana3-PA

ana3 
C-terminal domain
necessary binding region
aa 1195-1590

coordinates relative to ana3-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Panda et al., 2020)
FBrf0245965-4.PD.WB
Reference
Panda et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ana3 

Ana3

bait

GST tag

ana3 
ana3 
Rcd4 

Rcd4

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ana3 
C-terminal domain
sufficient binding region
aa 1195-1977

coordinates relative to ana3-PA

ana3 
C-terminal domain
necessary binding region
aa 1195-1590

coordinates relative to ana3-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in yeast system; prey produced as a recombinant fusion protein in yeast system.

(Panda et al., 2020)
External Crossreferences and Linkouts ( 0 )
References (1)