Gorab and Sas-6 form a trimeric complex in solution with stoichiometry 2Sas-6 :1Gorab.

Interaction in vitro; proteins produced as recombinant fusion proteins in bacterial system.

Comparison of HDX patterns of Gorab or Sas-6 alone and in complex with each other predicts the aa 230-260 segment of Gorab and aa 440-460 of Sas-6 as involved in the interaction.

Interaction in vitro; proteins produced as recombinant fusion proteins in bacterial system.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

Kd = 47nM

Gorab appears to bind the Sas-6 dimer in an antiparallel manner.

Interaction in vitro; proteins produced as recombinant fusion proteins in bacterial system.

Bait protein was fused to an outer mitochondrial membrane anchor, which targets the chimera to mitochondria. The ability of this chimera to recruit prey protein to mitochondria was taken as evidence of direct physical interaction.

Source was S2 cell line; bait produced from tagged transfected construct; prey produced from transfected construct.

Bait protein was fused to an outer mitochondrial membrane anchor, which targets the chimera to mitochondria. The ability of this chimera to recruit prey protein to mitochondria was taken as evidence of direct physical interaction.

Source was S2 cell line; bait produced from tagged transfected construct; prey produced from endogenous gene.