Plk4
member of the polo-like serine/threonine protein kinase family - required for centriole duplication and for formation of basal bodies and flagella
Please see the JBrowse view of Dmel\SAK for information on other features
To submit a correction to a gene model please use the Contact FlyBase form
AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.45
There is only one protein coding transcript and one polypeptide associated with this gene
Homodimer (By similarity). Interacts with Alms1a (PubMed:32965218).
Ubiquitinated by the SCF-slmb ubiquitin ligase complex; leading to its degradation by the proteasome during interphase and regulating centriole number and ensuring the block to centriole reduplication.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\SAK using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
Comment: maternally deposited
JBrowse - Visual display of RNA-Seq signals
View Dmel\SAK in JBrowse
3-47
3-43.8
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
New stable cell line derived from S2-ThermoFischer : A CRISPR method for S2 cells that can be adapted for both knock-in and knock-out of any gene was developed. The followind lines were created (author designations): S2-Cas9Par, S2-Plk4 CRISPR null line 1, S2-Plk4 CRISPR null line 2.2, S2-Plk4 CRISPR null line 7, S2-Ana2 CRISPR null line 2, S2-Ana2 CRISPR null line 4, S2-Ana2 CRISPR null line 5, S2-ana2 CRISPR null line 12, Ana2 CRISPR null line 15, and Ana2 CRISPR null line 17. Note that Plk4 is SAK in FlyBase and Ana2 is ana2 in FlyBase.
SAK accumulates on centrioles throughout the cell cycle and promotes excess daughter centriole formation.
SAK is involved in centriole duplication.
RNAi screen using dsRNA made from templates generated with primers directed against this gene results in the spindles that are either anastral (no γ-tubulin staining at the poles) or monastral (only one pole with γ-tubulin staining) when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.
S2 cells transfected with dsRNA made from templates generated with primers directed against this gene show a loss of centrioles. Such cells are able to undergo repeated rounds of cell division, but display broad disorganized mitotic spindle poles.
When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, a whole range of mitotic abnormalities, centrosome abnormalities and abnormal spindles are seen.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
