Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from transfected construct.
Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from transfected construct.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
K22M, one of three mutations in kinase-dead form
K43M, one of three mutations in kinase-dead form
D156A, one of three mutations in kinase-dead form
K22M, one of three mutations in kinase-dead form
K43M, one of three mutations in kinase-dead form
D156A, one of three mutations in kinase-dead form
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
K22M, one of three mutations in the kinase dead (KD) mutant
K43M, one of three mutations in the kinase dead (KD) mutant
D146A, one of three mutations in the kinase dead (KD) mutant
S-to-A mutations, excluding S293 in Slimb degron DSGIIT
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Phosphorylation of the phospho cluster around the Slimb degron primes Thr297 phosphorylation, but does not affect Ser293 phosphorylation.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.
