FlyBase Gene Report: Dmel\Sik2

General Information

Symbol

Dmel\Sik2

Species

D. melanogaster

Name

Salt-inducible kinase 2

Annotation Symbol

CG4290

Feature Type

protein_coding_gene

FlyBase ID

FBgn0025625

Gene Model Status

Current

Stock Availability

11 publicly available

Gene Summary

Salt-inducible kinase 2 (Sik2) encodes a serine/threonine kinase that is activated by the product of Lkb1. It regulates lipid storage and energy homeostasis by controlling the activity of cAMP-response element-binding protein (CREB)-regulated transcription coactivator (CRTC). [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Pathway
Gene Group
Interactive Fly

Also Known As

EG:22E5.8 , SIK, Salt-induced kinase 2

Key Links

Genomic Location

Cytogenetic map

2C8-2C8

Sequence location

X:2,055,310..2,061,915 [-]

Recombination map

1-0.5

RefSeq locus

NC_004354 REGION:2055310..2061915

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (10 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables protein serine/threonine kinase activity

inferred from mutant phenotype

(Wang et al., 2008)

inferred from direct assay

(Wehr et al., 2013, Choi et al., 2011)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR017441

(InterPro Project Members, 2004-)

enables protein kinase activity

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR008271

(InterPro Project Members, 2004-)

enables protein serine/threonine kinase activity

inferred from electronic annotation with InterPro:IPR034672

(InterPro Project Members, 2004-)

Biological Process (5 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

involved_in cellular response to insulin stimulus

inferred from genetic interaction with FLYBASE:Akt; FB:FBgn0010379

(Wang et al., 2008)

involved_in insulin receptor signaling pathway

inferred from mutant phenotype

(Wang et al., 2008)

involved_in negative regulation of hippo signaling

inferred from mutant phenotype

(Wehr et al., 2013)

involved_in response to starvation

inferred from mutant phenotype

(Choi et al., 2011)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in intracellular signal transduction

inferred from biological aspect of ancestor with PANTHER:PTN008613719

(GO Reference Genome Project, 2011-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN001552066

(GO Reference Genome Project, 2011-)

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN001552066

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASES

Pathway (FlyBase)

INSULIN-LIKE RECEPTOR SIGNALING PATHWAY CORE COMPONENTS

NEGATIVE REGULATORS OF HIPPO SIGNALING PATHWAY

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASES -: The Calcium/Calmodulin-dependent kinase family are serine/threonine kinases which, in general, prefer substrates containing basic residues. Many, but not all, members are regulated by calcium or calmodulin. (Adapted from FBrf0132098).

Pathway (FlyBase)

INSULIN-LIKE RECEPTOR SIGNALING PATHWAY CORE COMPONENTS -: The Insulin-like Receptor (IR) signaling pathway in Drosophila is initiated by the binding of an insulin-like peptides to the Insulin-like receptor (InR). (Adapted from FBrf0232297, FBrf0230017 and FBrf0229989.)
NEGATIVE REGULATORS OF HIPPO SIGNALING PATHWAY -: The Hippo signaling pathway is an intracellular kinase cascade in which hpo kinase in complex with sav, phosphorylates wts kinase which, in turn, phosphorylates yki transcriptional co-activator leading to its cytosolic retention. Negative regulators of the pathway promote the nuclear accumulation of yki, enhancing yki-mediated transcriptional regulation and the expression of genes that positively regulate tissue growth (Adapted from FBrf0224870).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Sik2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9W532)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.50

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Sik2-RA

FBtr0070340

NM_130616

5230

1398

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Sik2-PA

FBpp0070326

149.9

1398

8.53

Q9W532

NP_569972

AAF45711

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Sik2 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Sik2_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.61

Transcript Expression

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

larval brain

(Choi et al., 2011)

imaginal disc

(Choi et al., 2011)

embryonic/larval fat body

(Choi et al., 2011)

embryonic/larval Malpighian tubule

(Choi et al., 2011)

embryonic/larval salivary gland

(Choi et al., 2011)

embryonic/larval midgut

(Choi et al., 2011)

adult stage

adult head

(Choi et al., 2011)

thorax

(Choi et al., 2011)

abdomen

(Choi et al., 2011)

adult stage | male

(Choi et al., 2011)

adult stage | female

(Choi et al., 2011)

Additional Descriptive Data

Sik2 transcripts are observed in a number of tissues in third instar larvae with the highest levels in the brain. They are observed in both males and females adults and the highest levels are in the head.

(Choi et al., 2011)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

Reporter: P{Sik2-GAL4.L}

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult Dh44 neuron of pars intercerebralis

(Lee et al., 2015)

adult brain | restricted

(Lee et al., 2015)

adult ventral nerve cord | restricted

(Lee et al., 2015)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Sik2 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Sik2

View exonic expression by tissue for Dmel\Sik2

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0025625

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

31170/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0025625

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0025625

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0025625

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4290

Flygut - An atlas of the Drosophila adult midgut

FBgn0025625

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 6 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 13 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Sik2

Transgenic constructs containing regulatory region of Sik2

Aberrations (Deficiencies and Duplications) ( 4 )

Inferred from experimentation ( 4 )

Gene disrupted in

Df(1)Exel6289

(Ryder, 2004.4.26)

Df(1)moody-Δ17

(Bainton et al., 2005)

Df(1)moody-Δ18

(Bainton et al., 2005)

Gene partially disrupted in

Df(1)Sik2¹⁰

(Fayyazuddin, 2018.8.14)

Inferred from location ( 25 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

Sik2^Δ41

viable, with Scer\GAL4^Mef2.PR

Sik2^GD11275

viable, with Scer\GAL4^pnr-MD237

Sik2^GD11275

viable, with Scer\GAL4^tin.CΔ4

Sik2^GD11275

Other Phenotypes

Allele

abnormal stress response, with Scer\GAL4^AD.elav

Sik2^GD11275

decreased size | adult stage, with Scer\GAL4^ey.PH

Sik2^KK102292

decreased size | adult stage, with Scer\GAL4^GMR.long, Scer\GAL4^ey.PH

Sik2^KK102292

increased size | larval stage, with Scer\GAL4^GMR.long, Scer\GAL4^ey.PH

Sik2^KK102292

long lived | calorie restriction conditional

Sik2^Δ41

visible | adult stage, with Scer\GAL4^ey.PH

Sik2^KK102292

visible | adult stage, with Scer\GAL4^GMR.long, Scer\GAL4^ey.PH

Phenotype manifest in

Allele

antennal disc | larval stage, with Scer\GAL4^GMR.long, Scer\GAL4^ey.PH

Sik2^KK102292

eye, with Scer\GAL4^ey.PH

Sik2^KK102292

eye, with Scer\GAL4^GMR.long, Scer\GAL4^ey.PH

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (117)

Hsap\SIK2

11 of 14

Yes

Hsap\SIK1B

10 of 14

Yes

Hsap\SIK1

10 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

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1 of 14

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1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\PIM2

1 of 14

Yes

Hsap\PIM3

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

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1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (78)

Rnor\Sik1

10 of 14

Yes

Rnor\Sik2

10 of 14

Yes

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

Yes

Rnor\RGD1562638

2 of 14

Yes

Rnor\Sik3

2 of 14

Rnor\Smok2a

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\MAST1

1 of 14

Rnor\Mast4

1 of 14

Rnor\Melk

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

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1 of 14

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1 of 14

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1 of 14

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1 of 14

1 of 14

1 of 14

1 of 14

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1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Tssk6

1 of 14

Rnor\Ttk

1 of 14

Mus musculus (laboratory mouse) (89)

Mmus\Sik2

11 of 14

Yes

Mmus\Sik1

10 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

2 of 14

Yes

Mmus\4921509C19Rik

2 of 14

Yes

Mmus\Gm4567

2 of 14

Yes

Mmus\Gm4922

2 of 14

Yes

Mmus\Gm5157

2 of 14

Yes

Mmus\Gm5891

2 of 14

Yes

Mmus\Gm6176

2 of 14

Yes

Mmus\Gm6882

2 of 14

Yes

Mmus\Gm7168

2 of 14

Yes

Mmus\Gm10662

2 of 14

Yes

Mmus\Gm10668

2 of 14

Yes

Mmus\Gm14147

2 of 14

Yes

Mmus\Gm14151

2 of 14

Yes

Mmus\Sik3

2 of 14

Mmus\Smok2a

2 of 14

Yes

Mmus\Smok2b

2 of 14

Yes

Mmus\Smok3a

2 of 14

Mmus\Smok3b

2 of 14

Mmus\Smok3c

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Gm4565

1 of 14

Yes

Mmus\Gm5890

1 of 14

Yes

Mmus\Gm6902

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

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1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Tssk6

1 of 14

Mmus\Ttk

1 of 14

Xenopus tropicalis (Western clawed frog) (60)

Xtro\sik2

6 of 13

Yes

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (77)

Drer\sik2a

11 of 14

Yes

Drer\sik2b

11 of 14

Yes

Drer\sik1

9 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

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1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Drer\si:ch211-171h4.3

1 of 14

Drer\si:dkey-8e10.3

1 of 14

Drer\si:zfos-2326c3.2

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (62)

Cele\kin-29

3 of 14

Yes

Cele\K02E10.7

2 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

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1 of 14

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1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

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1 of 14

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1 of 14

1 of 14

1 of 14

1 of 14

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1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Anopheles gambiae (African malaria mosquito) (49)

Agam\AgaP_AGAP013439

10 of 12

Yes

Agam\AgaP_AGAP003005

9 of 12

Yes

3 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

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1 of 12

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1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (92)

Atha\AT1G22870

1 of 13

Yes

Atha\AT1G30640

1 of 13

Yes

Atha\AT1G49180

1 of 13

Yes

Atha\AT1G71410

1 of 13

Yes

Atha\AT2G20050

1 of 13

Yes

Atha\AT2G37840

1 of 13

Yes

Atha\AT3G53930

1 of 13

Yes

Atha\AT3G61960

1 of 13

Yes

Atha\AT4G33080

1 of 13

Yes

Atha\AT5G11850

1 of 13

Yes

Atha\AT5G21222

1 of 13

Yes

Atha\AT5G57565

1 of 13

Yes

Atha\ATN1

1 of 13

Yes

Atha\AUR1

1 of 13

Yes

Atha\AUR2

1 of 13

Yes

Atha\AUR3

1 of 13

Yes

Atha\CDPK2

1 of 13

Yes

Atha\CDPK6

1 of 13

Yes

Atha\CDPK9

1 of 13

Yes

Atha\CIPK1

1 of 13

Yes

Atha\CIPK2

1 of 13

Yes

Atha\CIPK3

1 of 13

Yes

Atha\CIPK4

1 of 13

Yes

Atha\CIPK5

1 of 13

Yes

Atha\CIPK7

1 of 13

Yes

Atha\CIPK8

1 of 13

Yes

Atha\CIPK9

1 of 13

Yes

Atha\CIPK12

1 of 13

Yes

Atha\CIPK13

1 of 13

Yes

Atha\CIPK15

1 of 13

Yes

Atha\CIPK16

1 of 13

Yes

Atha\CIPK17

1 of 13

Yes

Atha\CIPK18

1 of 13

Yes

Atha\CIPK19

1 of 13

Yes

Atha\CIPK20

1 of 13

Yes

Atha\CIPK21

1 of 13

Yes

Atha\CIPK22

1 of 13

Yes

Atha\CIPK23

1 of 13

Yes

Atha\CIPK25

1 of 13

Yes

Atha\CIPK26

1 of 13

Yes

Atha\CPK1

1 of 13

Yes

Atha\CPK2

1 of 13

Yes

Atha\CPK4

1 of 13

Yes

Atha\CPK9

1 of 13

Yes

Atha\CPK13

1 of 13

Yes

Atha\CPK14

1 of 13

Yes

Atha\CPK15

1 of 13

Yes

Atha\CPK16

1 of 13

Yes

Atha\CPK17

1 of 13

Yes

Atha\CPK18

1 of 13

Yes

Atha\CPK19

1 of 13

Yes

Atha\CPK20

1 of 13

Yes

Atha\CPK23

1 of 13

Yes

Atha\CPK27

1 of 13

Yes

Atha\CPK28

1 of 13

Yes

Atha\CPK29

1 of 13

Yes

Atha\CPK31

1 of 13

Yes

Atha\CPK32

1 of 13

Yes

Atha\CPK33

1 of 13

Yes

Atha\CPK34

1 of 13

Yes

Atha\CTEXP

1 of 13

Yes

Atha\FU

1 of 13

Yes

Atha\GRIK1

1 of 13

Yes

Atha\GRIK2

1 of 13

Yes

Atha\GSK1

1 of 13

Yes

Atha\KIN10

1 of 13

Yes

Atha\KIN11

1 of 13

Yes

Atha\MPK16

1 of 13

Yes

Atha\NEK2

1 of 13

Yes

Atha\NEK3

1 of 13

Yes

Atha\NEK6

1 of 13

Yes

Atha\OST1

1 of 13

Yes

Atha\PEPKR2

1 of 13

Yes

Atha\PK3

1 of 13

Yes

Atha\PPCK2

1 of 13

Yes

Atha\SIK1

1 of 13

Yes

Atha\SIP1

1 of 13

Yes

Atha\SIP3

1 of 13

Yes

Atha\SIP4

1 of 13

Yes

Atha\SKdZeta

1 of 13

Yes

Atha\SnRK1.3

1 of 13

Yes

Atha\SNRK2-8

1 of 13

Yes

Atha\SNRK2.1

1 of 13

Yes

Atha\SNRK2.2

1 of 13

Yes

Atha\SNRK2.3

1 of 13

Yes

Atha\SNRK2.4

1 of 13

Yes

Atha\SNRK2.5

1 of 13

Yes

Atha\SNRK2.7

1 of 13

Yes

Atha\SNRK2.9

1 of 13

Yes

Atha\SNRK2.10

1 of 13

Yes

Atha\SOS2

1 of 13

Yes

Atha\YDA

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (43)

Scer\HAL5

2 of 13

Yes

Scer\KIN1

2 of 13

Yes

Scer\KIN2

2 of 13

Yes

Scer\KKQ8

2 of 13

Yes

Scer\NPR1

2 of 13

Yes

Scer\PRR2

2 of 13

Yes

Scer\PTK1

2 of 13

Yes

Scer\PTK2

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (19)

Spom\ark1

1 of 12

Yes

Spom\cdr1

1 of 12

Yes

Spom\hal4

1 of 12

Yes

Spom\kin1

1 of 12

Yes

Spom\mph1

1 of 12

Yes

Spom\nak1

1 of 12

Yes

Spom\oca2

1 of 12

Yes

Spom\orb6

1 of 12

Yes

Spom\ppk6

1 of 12

Yes

Spom\ppk9

1 of 12

Yes

Spom\ppk16

1 of 12

Yes

Spom\ppk27

1 of 12

Yes

Spom\ppk29

1 of 12

Yes

Spom\ppk30

1 of 12

Yes

Spom\ppk32

1 of 12

Yes

Spom\ppk34

1 of 12

Yes

Spom\ppk38

1 of 12

Yes

Spom\SPCC70.05c

1 of 12

Yes

Spom\ssp2

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Sik2. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (48)

4 of 13

3 of 13

3 of 13

3 of 13

sff

3 of 13

3 of 13

3 of 13

3 of 13

2 of 13

grp

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

Akt

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Csk

1 of 13

for

1 of 13

hpo

1 of 13

inaC

1 of 13

lok

1 of 13

mbt

1 of 13

Mekk1

1 of 13

meng

1 of 13

Nak

1 of 13

niki

1 of 13

Pak3

1 of 13

Pak

1 of 13

Pask

1 of 13

PhKγ

1 of 13

Pkc53E

1 of 13

PKD

1 of 13

Pkg21D

1 of 13

S6kII

1 of 13

SAK

1 of 13

Src64B

1 of 13

Strn-Mlck

1 of 13

yata

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

SIK1; salt inducible kinase 1

model of developmental and epileptic encephalopathy 30

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 5 )

Allele

Disease

Interaction

References

Sik2^HMC04153

exacerbates atypical teratoid rhabdoid tumor

modeled by Snr1^KK101602

(Tegeder et al., 2019)

ameliorates cancer

modeled by Hipk^UAS.Tag:HA

(Yu et al., 2023)

Sik2^UAS.Tag:MYC

exacerbates cancer

modeled by Hipk^UAS.Tag:HA

(Yu et al., 2023)

Sik2^S1032A.UAS

exacerbates carcinoma

modeled by Csk^Q156Stop, Ras85D^V12.UAS

(Hirabayashi and Cagan, 2015)

exacerbates cancer

modeled by Hipk^UAS.Tag:HA

(Yu et al., 2023)

Sik2^WT.UAS

exacerbates cancer

modeled by Hipk^UAS.Tag:HA

(Yu et al., 2023)

Sik2^RNAi.UAS.cUa

ameliorates carcinoma

modeled by Csk^Q156Stop, Ras85D^V12.UAS

(Hirabayashi and Cagan, 2015)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

SIK2; salt inducible kinase 2

11 of 14

608973

Hsap\SIK1B

10 of 14

SIK1; salt inducible kinase 1

10 of 14

605705

DEVELOPMENTAL AND EPILEPTIC ENCEPHALOPATHY 30; DEE30

developmental and epileptic encephalopathy 30

MARK1; microtubule affinity regulating kinase 1

3 of 14

606511

MARK2; microtubule affinity regulating kinase 2

3 of 14

600526

INTELLECTUAL DEVELOPMENTAL DISORDER, AUTOSOMAL DOMINANT 76; MRD76

MARK3; microtubule affinity regulating kinase 3

3 of 14

MAP/MICROTUBULE AFFINITY-REGULATING KINASE 3; MARK3

VISUAL IMPAIRMENT AND PROGRESSIVE PHTHISIS BULBI; VIPB

visual impairment and progressive phthisis bulbi

MARK4; microtubule affinity regulating kinase 4

3 of 14

606495

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Sik2 - Crtc

protein kinase assay, genetic interference, anti tag western blot

(Choi et al., 2011)

Sik2 - Hipk

anti tag coimmunoprecipitation, western blot, anti bait coimmunoprecipitation, anti tag western blot

(Yu et al., 2023)

Sik2 - Mer

anti tag coimmunoprecipitation, anti tag western blot

(Wehr et al., 2013)

Sik2 - sav

anti tag coimmunoprecipitation, anti tag western blot

(Wehr et al., 2013)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Sik2

suppressible

Crtc

(Choi et al., 2011)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

brm

suppressible

Sik2

(Tegeder et al., 2019)

Crtc

suppressible

Sik2

(Choi et al., 2011)

Delta

enhanceable

Sik2

(Şahin et al., 2020)

fng

enhanceable

Sik2

(Şahin et al., 2020)

Ser

enhanceable

Sik2

(Şahin et al., 2020)

Snr1

suppressible

Sik2

(Tegeder et al., 2019)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

57714

DroID - A comprehensive database of gene and protein interactions.

FBgn0025625

MIST (protein-protein) - An integrated Molecular Interaction Database

31170

Pathways

Signaling Pathways (FlyBase)

INSULIN-LIKE RECEPTOR SIGNALING PATHWAY CORE COMPONENTS
NEGATIVE REGULATORS OF HIPPO SIGNALING PATHWAY

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

O77268

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-0.5

Cytogenetic map

2C8-2C8

Sequence location

X:2,055,310..2,061,915 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

2C8-2C8

Limits computationally determined from genome sequence between P{EP}Actn^EP1193&P{EP}CG4322^EP1631 and P{EP}EP1460&P{EP}CG2924^EP1596

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

1-0.5

(FlyBase, 2016)

1-0.2

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (11)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

79017

Sik2¹³ w^*

79023

w^*; P{UAS-Sik2.F}attP2

33449

P{EP}Sik2^G366 w^*

55880

y¹ v¹; P{TRiP.HMC04153}attP2

605425

y¹ TI{CRIMIC.TG4.1}Sik2^{CR71226-TG4.1} w^*

10651

10718

HMJ23210

y¹ v¹; P{TRiP.HMJ23210}attP40/CyO

VDRC

v103739

P{KK102292}VIE-260B

v26497

w¹¹¹⁸; P{GD11275}v26497

v341018

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD00021}attP40/CyO-GFP

Genomic Clones (8)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (7)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RH42017

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0025625

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RH67885

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0025625

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: Sik2 CG4290

(Wang et al., 2008)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

CG4290

(National Institute of Genetics Fly Stocks, 2023-, Couderc et al., 2017, Hosono et al., 2015, Wehr et al., 2013, Abruzzi et al., 2011, Wang et al., 2011, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Wang et al., 2008, Quinones-Coello, 2007, Comet et al., 2006, Bainton et al., 2005)

EG:22E5.8

(Giot et al., 2003, Benos et al., 2001)

SIK

(Lam Wong and Verheyen, 2021, Choi et al., 2011)

SIK2

(Couderc et al., 2017, Kim et al., 2016, Wang et al., 2011)

Sik2

(Khan et al., 2021, Ostalé et al., 2021, Şahin et al., 2020, Theis et al., 2020, Tegeder et al., 2019, Gene Disruption Project members, 2018-, Yadav et al., 2016, Tipping and Perrimon, 2014, Wehr et al., 2013)

sik2

(Wang et al., 2021, Hirabayashi and Cagan, 2015)

Name Synonyms

Salt-induced kinase 2

(Wang et al., 2008)

Salt-inducible kinase 2

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 38 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

31170

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9W532

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9W532

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0025625

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

31170/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0025625

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0025625

FlyMine - An integrated database for Drosophila genomics

FBgn0025625

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG4290

MARRVEL_MODEL - MARRVEL (model organism gene)

31170

Linkouts

BioGRID - A database of protein and genetic interactions.

57714

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0025625

DroID - A comprehensive database of gene and protein interactions.

FBgn0025625

DRSC - Results frm RNAi screens

FBgn0025625

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Sik2_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0025625

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0025625

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4290

Flygut - An atlas of the Drosophila adult midgut

FBgn0025625

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC007481

MIST (protein-protein) - An integrated Molecular Interaction Database

31170

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

O77268

References (67)

The following external sites may use different assemblies or annotations than FlyBase.

-0.61

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