FlyBase Gene Report: Dmel\Nuak

General Information

Symbol

Dmel\Nuak

Species

D. melanogaster

Name

Nuak family kinase

Annotation Symbol

CG43143

Feature Type

protein_coding_gene

FlyBase ID

FBgn0262617

Gene Model Status

Current

Stock Availability

23 publicly available

Enzyme Name (EC)

Gene Summary

serine/threonine kinase that biochemically and genetically interacts with the cochaperone Starvin in the autophagic clearance of protein aggregates in larval muscles - Filamin/Cheerio is a target of NUAK kinase activity and abnormally accumulates upon loss of the Starvin-Hsc70-4 complex (Interactive Fly, Nuak )

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

Nuak1

Key Links

Genomic Location

Cytogenetic map

86A3-86A7

Sequence location

3R:10,264,306..10,308,467 [+]

Recombination map

3-49

RefSeq locus

NT_033777 REGION:10264306..10308467

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (6 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR017441

(InterPro Project Members, 2004-)

enables protein kinase activity

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR008271

(InterPro Project Members, 2004-)

enables protein serine/threonine kinase activity

inferred from sequence or structural similarity with UniProtKB:O60285

(Gene Ontology Curators, 2002-)

enables structural molecule activity

inferred from electronic annotation with InterPro:IPR017404

(InterPro Project Members, 2004-)

Biological Process (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from sequence or structural similarity with UniProtKB:O60285

(Gene Ontology Curators, 2002-)

located_in nucleus

inferred from sequence or structural similarity with UniProtKB:O60285

(Gene Ontology Curators, 2002-)

Gene Group (FlyBase)

CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASES

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

protein serine/threonine kinase activity

Summaries

Gene Group (FlyBase)

CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASES -: The Calcium/Calmodulin-dependent kinase family are serine/threonine kinases which, in general, prefer substrates containing basic residues. Many, but not all, members are regulated by calcium or calmodulin. (Adapted from FBrf0132098).

Summary (Interactive Fly)

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Nuak for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry A0A6H2EG35)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.48

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Gene model reviewed during 5.49

Stop-codon suppression (UGA) postulated; FBrf0243886.

Gene model reviewed during 6.32

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Nuak-RA

FBtr0305352

NM_141734

4588

1427

Nuak-RB

FBtr0305354

NM_169337

5076

1427

Nuak-RC

FBtr0305353

NM_206470

4793

1427

Nuak-RD

FBtr0305351

NM_206469

5997

1180

Nuak-RE

FBtr0305355

NM_141736

8836

2537

Nuak-RF

FBtr0305356

NM_001260090

5918

1532

Nuak-RG

FBtr0305357

NM_001260091

5566

1199

Nuak-RH

FBtr0337049

NM_001275528

5525

1551

Nuak-RI

FBtr0337050

NM_001275529

9381

2556

Nuak-RJ

FBtr0479807

NM_001382098

9324

2618

Nuak-RK

FBtr0479808

NM_001382099

5177

1285

Nuak-RL

FBtr0479809

NM_001382100

8874

2537

Nuak-RM

FBtr0479810

NM_001382101

10122

2803

Nuak-RN

FBtr0479811

NM_001382102

9324

2537

Nuak-RO

FBtr0479812

NM_001382103

5975

1551

Nuak-RP

FBtr0479813

NM_001382104

5468

1532

Nuak-RQ

FBtr0479814

NM_001382105

4727

1285

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Nuak-PA

FBpp0293891

153.4

1427

9.08

FBpp0293893

153.4

1427

9.08

FBpp0293892

153.4

1427

9.08

FBpp0293890

127.7

1180

8.33

FBpp0293894

274.9

2537

9.94

FBpp0293895

163.9

1532

9.50

FBpp0293896

130.0

1199

8.55

FBpp0307978

166.1

1551

9.57

FBpp0307979

277.2

2556

9.96

FBpp0428140

283.6

2618

9.97

QJC18451

Nuak-PK

FBpp0428141

138.2

1285

9.08

QJC18452

Nuak-PL

FBpp0428142

274.9

2537

9.94

Q9VH03

QJC18453

Nuak-PM

FBpp0428143

302.9

2803

9.99

QJC18454

Nuak-PN

FBpp0428144

274.9

2537

9.94

Q9VH03

QJC18455

Nuak-PO

FBpp0428145

166.1

1551

9.57

A0A0B4KGR1

QJC18456

Nuak-PP

FBpp0428146

163.9

1532

9.50

A0A0B4K707

QJC18457

Nuak-PQ

FBpp0428147

138.2

1285

9.08

QJC18458

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1427 aa isoforms: Nuak-PA, Nuak-PB, Nuak-PC

2537 aa isoforms: Nuak-PE, Nuak-PL, Nuak-PN

1532 aa isoforms: Nuak-PF, Nuak-PP

1551 aa isoforms: Nuak-PH, Nuak-PO

1285 aa isoforms: Nuak-PK, Nuak-PQ

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Nuak using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.43

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Nuak in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Nuak

View exonic expression by tissue for Dmel\Nuak

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

41256/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0262617

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0262617

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG11870

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 19 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 15 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Nuak

Transgenic constructs containing regulatory region of Nuak

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(3R)Exel6156

(Ryder, 2004.4.26)

Inferred from location ( 5 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

Nuak^K99R

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

Nuak^GD7410

lethal - all die during P-stage, with Scer\GAL4^Mef2.PU

Nuak^UAS.RD

partially lethal, with Scer\GAL4^tin.CΔ4

Nuak^GD7410

partially lethal - majority die, with Nuak^K99R, Scer\GAL4^Mef2.PR

Nuak^UAS.RD

partially lethal - majority die, with Nuak^UAS.RD, Scer\GAL4^Mef2.PR

Nuak^K99R

partially lethal - majority live, with Nuak^K99R, Scer\GAL4^Mef2.PR

Nuak^T226E.UAS

partially lethal - majority live, with Nuak^T226E.UAS, Scer\GAL4^Mef2.PR

Nuak^K99R

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Nuak^GD7411

viable, with Scer\GAL4^pnr-MD237

Sterility

Allele

female semi-sterile, with Scer\GAL4^{VP16.mat.αTub67C}

Nuak^GL00066

Other Phenotypes

Allele

abnormal body size | P-stage

Nuak¹⁷²⁸⁹

abnormal body size | P-stage, with Scer\GAL4^da.PU

abnormal body size | P-stage, with Scer\GAL4^how-24B

abnormal body size | P-stage, with Scer\GAL4^Mef2.PR

Nuak^UAS.RD

abnormal body size | P-stage, with Scer\GAL4^Mef2.PU

abnormal body size | P-stage (with Df(3R)BSC479)

Nuak¹⁷²⁸⁹

abnormal locomotor behavior | third instar larval stage

Nuak¹⁷²⁸⁹

abnormal locomotor behavior | third instar larval stage, with Scer\GAL4^Mef2.PR

Nuak^JF02162

decreased rate of adult locomotory behavior, with Scer\GAL4^Mef2.PU

Nuak^JF02162

decreased size | adult stage, with Scer\GAL4^en-e16E

Nuak^JF02162

decreased size | P-stage

Nuak¹⁷²⁸⁹

flightless, with Scer\GAL4^Mef2.PR

Nuak^GD7411

increased body size | P-stage, with Scer\GAL4^Mef2.PR

increased mortality during development | calorie restriction conditional

Nuak¹⁷²⁸⁹

majority die during embryonic stage

Nuak^K99R

majority die during P-stage

Nuak¹⁷²⁸⁹

short lived, with Scer\GAL4^Mef2.PU

Nuak^JF02162

some die during first instar larval stage

Nuak^K99R.UAS

visible | adult stage, with Scer\GAL4^en-e16E

Nuak^JF02162

Phenotype manifest in

Allele

actin filament | adult stage, with Scer\GAL4^Mef2.PU

Nuak^JF02162

anterior pupal spiracle | larval stage

Nuak¹⁷²⁸⁹

embryonic/larval hypodermal muscle cell | larval stage

Nuak¹⁷²⁸⁹

embryonic/larval hypodermal muscle cell | larval stage, with Scer\GAL4^Mef2.PR

Nuak^JF02162

embryonic/larval hypodermal muscle cell | third instar larval stage, with Scer\GAL4^Mef2.PR

Nuak^GL00066

endosome | larval stage, with Scer\GAL4^Mef2.PU

Nuak^JF02162

indirect flight muscle | adult stage, with Scer\GAL4^Mef2.PU

Nuak^JF02162

larval somatic muscle | larval stage, with Scer\GAL4^C57

larval somatic muscle | larval stage, with Scer\GAL4^G7

larval somatic muscle | larval stage, with Scer\GAL4^Mef2.PR

larval somatic muscle | larval stage, with Scer\GAL4^Mef2.PU

Nuak^JF02162

lysosome | larval stage, with Scer\GAL4^Mef2.PU

Nuak^JF02162

mitochondrial crista | larval stage

Nuak¹⁷²⁸⁹

mitochondrion | larval stage

Nuak¹⁷²⁸⁹

muscle cell of ventral longitudinal muscle 3 | larval stage

Nuak¹⁷²⁸⁹

muscle cell of ventral longitudinal muscle 3 | larval stage, with Scer\GAL4^Mef2.PR

Nuak^JF02162

myofibril, with Scer\GAL4^Mef2.PR

Nuak^GD7411

sarcomere, with Scer\GAL4^Mef2.PR

Nuak^GD7411

sarcomere | increased number | larval stage, with Scer\GAL4^Mef2.PR

Nuak^JF02162

sarcomere | larval stage, with Scer\GAL4^Mef2.PU

Nuak^JF02162

skeletal muscle myofibril | adult stage, with Scer\GAL4^Mef2.PU

Nuak^JF02162

ventral longitudinal muscle cell | larval stage

Nuak¹⁷²⁸⁹

wing posterior compartment, with Scer\GAL4^en-e16E

Nuak^JF02162

Z disc, with Scer\GAL4^Mef2.PR

Nuak^GD7411

Z disc | larval stage

Nuak¹⁷²⁸⁹

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (53)

Hsap\NUAK1

8 of 14

Yes

Hsap\NUAK2

8 of 14

Yes

Hsap\BRSK1

2 of 14

Hsap\BRSK2

2 of 14

Hsap\HUNK

2 of 14

Yes

Hsap\PRKAA1

2 of 14

Hsap\SIK1B

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\PIM2

1 of 14

Yes

Hsap\PIM3

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (51)

Rnor\Nuak1

8 of 14

Yes

Rnor\Nuak2

8 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Ulk1

1 of 14

Rnor\Ulk2

1 of 14

Rnor\Ulk3

1 of 14

Mus musculus (laboratory mouse) (64)

Mmus\Nuak1

8 of 14

Yes

Mmus\Nuak2

8 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Gm4565

1 of 14

Yes

Mmus\Gm4567

1 of 14

Mmus\Gm5157

1 of 14

Mmus\Gm5890

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Ulk1

1 of 14

Mmus\Ulk2

1 of 14

Mmus\Ulk3

1 of 14

Xenopus tropicalis (Western clawed frog) (32)

Xtro\LOC100145764

6 of 13

Yes

Xtro\LOC100485259

5 of 13

Yes

Xtro\LOC116406512

5 of 13

Yes

Xtro\nuak1

5 of 13

Yes

Xtro\nuak2

5 of 13

Yes

Xtro\aurka

1 of 13

Xtro\camk1

1 of 13

Xtro\cldn1

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\pim3

1 of 13

Yes

Xtro\prkaa1

1 of 13

Xtro\rflnb

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\snrk

1 of 13

Xtro\stk16

1 of 13

Xtro\stk35

1 of 13

Yes

Xtro\ulk1

1 of 13

Xtro\ulk3

1 of 13

Danio rerio (Zebrafish) (50)

Drer\nuak1a

8 of 14

Yes

Drer\nuak2

8 of 14

Yes

Drer\nuak1b

7 of 14

Yes

Drer\LOC101885029

4 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\LOC101885707

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Drer\si:ch73-122k23.1

1 of 14

Yes

Drer\si:ch211-27e6.1

1 of 14

Drer\si:ch211-63o20.7

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (31)

Cele\unc-82

4 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Anopheles gambiae (African malaria mosquito) (21)

Agam\AgaP_AGAP003174

7 of 12

Yes

Agam\AgaP_AGAP003175

2 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (51)

Atha\AT1G22870

1 of 13

Yes

Atha\AT1G71410

1 of 13

Yes

Atha\AT2G32850

1 of 13

Yes

Atha\AT2G37840

1 of 13

Yes

Atha\AT3G53930

1 of 13

Yes

Atha\AT3G61960

1 of 13

Yes

Atha\AT5G21222

1 of 13

Yes

Atha\AT5G57565

1 of 13

Yes

Atha\CAK4

1 of 13

Yes

Atha\CDKB1;2

1 of 13

Yes

Atha\CIPK1

1 of 13

Yes

Atha\CIPK2

1 of 13

Yes

Atha\CIPK3

1 of 13

Yes

Atha\CIPK4

1 of 13

Yes

Atha\CIPK5

1 of 13

Yes

Atha\CIPK7

1 of 13

Yes

Atha\CIPK8

1 of 13

Yes

Atha\CIPK9

1 of 13

Yes

Atha\CIPK12

1 of 13

Yes

Atha\CIPK13

1 of 13

Yes

Atha\CIPK15

1 of 13

Yes

Atha\CIPK16

1 of 13

Yes

Atha\CIPK17

1 of 13

Yes

Atha\CIPK18

1 of 13

Yes

Atha\CIPK19

1 of 13

Yes

Atha\CIPK20

1 of 13

Yes

Atha\CIPK21

1 of 13

Yes

Atha\CIPK22

1 of 13

Yes

Atha\CIPK23

1 of 13

Yes

Atha\CIPK25

1 of 13

Yes

Atha\CIPK26

1 of 13

Yes

Atha\CPK24

1 of 13

Yes

Atha\CTEXP

1 of 13

Yes

Atha\FU

1 of 13

Yes

Atha\KIN10

1 of 13

Yes

Atha\KIN11

1 of 13

Yes

Atha\MPK16

1 of 13

Yes

Atha\NEK6

1 of 13

Yes

Atha\OST1

1 of 13

Yes

Atha\PK3

1 of 13

Yes

Atha\PPCK2

1 of 13

Yes

Atha\SIK1

1 of 13

Yes

Atha\SIP1

1 of 13

Yes

Atha\SIP3

1 of 13

Yes

Atha\SIP4

1 of 13

Yes

Atha\SnRK1.3

1 of 13

Yes

Atha\SNRK2.1

1 of 13

Yes

Atha\SNRK2.2

1 of 13

Yes

Atha\SNRK2.4

1 of 13

Yes

Atha\SNRK2.10

1 of 13

Yes

Atha\SOS2

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (29)

Scer\FRK1

2 of 13

Yes

Scer\HAL5

2 of 13

Yes

Scer\KIN1

2 of 13

Yes

Scer\KIN4

2 of 13

Yes

Scer\KKQ8

2 of 13

Yes

Scer\PRR2

2 of 13

Yes

Scer\PTK1

2 of 13

Yes

Scer\PTK2

2 of 13

Yes

Scer\SNF1

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (13)

Spom\ark1

1 of 12

Yes

Spom\cds1

1 of 12

Yes

Spom\chk1

1 of 12

Yes

Spom\fin1

1 of 12

Yes

Spom\hal4

1 of 12

Yes

Spom\ppk6

1 of 12

Yes

Spom\ppk8

1 of 12

Yes

Spom\ppk24

1 of 12

Yes

Spom\ppk29

1 of 12

Yes

Spom\ppk30

1 of 12

Yes

Spom\ppk38

1 of 12

Yes

Spom\SPCC70.05c

1 of 12

Yes

Spom\ssp2

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Nuak. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (26)

AMPKα

2 of 13

CG4629

2 of 13

sff

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

aux

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

grp

1 of 13

KP78a

1 of 13

KP78b

1 of 13

lok

1 of 13

Nak

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 2 )

Allele

Disease

Interaction

References

Nuak^GD7410

ameliorates tauopathy

modeled by Hsap\MAPT^UAS.cUa

(Lasagna-Reeves et al., 2016)

Nuak^EY22355

exacerbates tauopathy

modeled by Hsap\MAPT^UAS.cUa

(Lasagna-Reeves et al., 2016)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

NUAK1; NUAK family kinase 1

8 of 14

608130

NUAK2; NUAK family kinase 2

8 of 14

608131

ANENCEPHALY 2; ANPH2

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Nuak - Prm

two hybrid

(Zhao et al., 2023)

Nuak - Strip

anti tag coimmunoprecipitation, western blot, proximity ligation assay, fluorescence microscopy

(Guo et al., 2023)

Nuak - cher

two hybrid

(Brooks et al., 2020)

Nuak - l(2)efl

two hybrid, proximity ligation assay, fluorescence microscopy

(Zhao et al., 2023)

Nuak - stv

two hybrid

(Brooks et al., 2020)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Nuak

enhanceable

NdufA3

(Pueyo et al., 2023)

Nuak

enhanceable

stv

(Brooks et al., 2020)

Nuak

suppressible

stv

(Brooks et al., 2020)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Atg8a

enhanceable

Nuak

(Brooks et al., 2020)

enhanceable

(Guo et al., 2023)

enhanceable

(Guo et al., 2023)

enhanceable

(Guo et al., 2023)

enhanceable

(Brooks et al., 2020)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0262617

MIST (protein-protein) - An integrated Molecular Interaction Database

41256

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q0KI87

Class of Gene

gene_with_stop_codon_read_through

(FlyBase Genome Annotators, 2020, FlyBase Genome Annotators, 2019-)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-49

Cytogenetic map

86A3-86A7

Sequence location

3R:10,264,306..10,308,467 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

86A3-86A7

Limits computationally determined from genome sequence between P{EP}Fmr1^EP3517&P{PZ}tws⁰²⁴¹⁴ and P{PZ}jumu⁰⁶⁴³⁹&P{lacW}l(3)j8B6^j8B6

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-49

(FlyBase, 2016)

3-46.6

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (23)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

23429

w¹¹¹⁸; Mi{ET1}Nuak^MB02272

31885

y¹ v¹; P{TRiP.JF02162}attP2

63988

y¹ v¹; P{TRiP.HMJ30301}attP40

62742

w¹¹¹⁸; PBac{IT.GAL4}Nuak^0259-G4

36443

y¹ w^*; Mi{MIC}Nuak^MI02494

11870R-3

11871R-1

11871R-2

11872R-2

HMJ30301

y¹ v¹; P{TRiP.HMJ30301}attP40/CyO

VDRC

v106088

P{KK107440}VIE-260B

v45401

w¹¹¹⁸; P{GD7411}v45401

More stocks available...

Genomic Clones (19)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (69)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0262617

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Relationship to Other Genes

Source for database merge of

Source for merge of: CG11870 CG11871

(FlyBase Genome Annotators, 2002-2003)

Source for merge of: CG11870 CG11872

(FlyBase Genome Annotators, 2011)

Additional comments

Annotations CG11870 and CG11872 merged as CG43143 in release 5.33 of the genome annotation.

(FlyBase Genome Annotators, 2011)

Annotations CG11870 and CG11871 merged as CG11870 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Nomenclature History

Source for database identify of

Source for identity of: Nuak1 CG43143

(Lasagna-Reeves et al., 2016)

Source for identity of: Nuak Nuak1

(Brooks et al., 2020)

Nomenclature comments

FlyBase curator comment: The D. melanogaster genome appears to contain a single ortholog of the mammalian NUAK1 and NUAK2 genes (represented by the CG43143 annotation). For this reason the gene corresponding to the CG43143 annotation has been renamed to 'Nuak' (from 'Nuak1').

(Brooks et al., 2020)

Etymology

Gene is named 'Nuak1' based on orthology to human gene.

(Lasagna-Reeves et al., 2016)

Synonyms and Secondary IDs (12)

Reported As

Symbol Synonym

CG11870

(Wu et al., 2020, Couderc et al., 2017, Pogson et al., 2014, Japanese National Institute of Genetics, 2012.5.21, Glatter et al., 2011, Ni et al., 2011.7.19, Keleman et al., 2009.8.5, Perkins et al., 2009)

CG11871

(Amin et al., 2009, Luschnig, 2003.12.17)

CG11872

(Japanese National Institute of Genetics, 2012.5.21)

CG43143

(El Fissi et al., 2024, Brooks et al., 2020, Abruzzi et al., 2017, Clandinin and Owens, 2016-, Gene Disruption Project members, 2016-, Lasagna-Reeves et al., 2016, Bonnay et al., 2014, Sopko et al., 2014, FlyBase Genome Annotators, 2011, Perkins et al., 2009)

EMK/KIAA0537

(Morrison et al., 2000)

NUAK

(Pueyo et al., 2023, Zhao et al., 2023, Brooks and Geisbrecht, 2022, Brooks et al., 2020, Couderc et al., 2017, Amin et al., 2009)

Nuak

(Ostalé et al., 2021)

Nuak1

(FlyBase Genome Annotators, 2020, FlyBase Genome Annotators, 2019-, Mathiyalagan et al., 2019)

Omphk1

(Hirano et al., 2006)

Name Synonyms

Nuak family kinase

Nuak family kinase 1

Nuak1 ortholog

Secondary FlyBase IDs

FBgn0037804
FBgn0037805
FBgn0037806

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 89 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

41256

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

A0A6H2EG35

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

A0A6H2EG35

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

41256/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0262617

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0262617

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG43143

MARRVEL_MODEL - MARRVEL (model organism gene)

41256

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0262617

DroID - A comprehensive database of gene and protein interactions.

FBgn0262617

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0262617

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG11870

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC012367

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (protein-protein) - An integrated Molecular Interaction Database

41256

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q0KI87

References (74)

The following external sites may use different assemblies or annotations than FlyBase.

0.43

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