FlyBase Gene Report: Dmel\Cka

General Information

Symbol

Dmel\Cka

Species

D. melanogaster

Name

Connector of kinase to AP-1

Annotation Symbol

CG7392

Feature Type

protein_coding_gene

FlyBase ID

FBgn0044323

Gene Model Status

Current

Stock Availability

21 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Pathway
Gene Group
Interactive Fly

Also Known As

l(2)05836, WD-40-family-member

Key Links

Genomic Location

Cytogenetic map

28D3-28D3

Sequence location

2L:8,030,721..8,040,826 [+]

Recombination map

2-29

RefSeq locus

NT_033779 REGION:8030721..8040826

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (18 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables protein binding

inferred from physical interaction with FLYBASE:bsk; FB:FBgn0000229

(Chen et al., 2002)

inferred from physical interaction with FLYBASE:Jra; FB:FBgn0001291

(Chen et al., 2002)

inferred from physical interaction with FLYBASE:kay; FB:FBgn0001297

(Chen et al., 2002)

inferred from physical interaction with FLYBASE:hep; FB:FBgn0010303

(Chen et al., 2002)

inferred from physical interaction with UniProtKB:Q9VRR2

(Xu et al., 2023)

enables protein kinase binding

inferred from physical interaction with FLYBASE:hep; FB:FBgn0010303

(La Marca et al., 2019)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables protein-macromolecule adaptor activity

inferred from biological aspect of ancestor with PANTHER:PTN000402124

(GO Reference Genome Project, 2011-)

Biological Process (12 terms)

Terms Based on Experimental Evidence (11 terms)

CV Term

Evidence

References

involved_in dorsal closure

inferred from mutant phenotype

(Chen et al., 2002)

involved_in gravitaxis

inferred from mutant phenotype

(Armstrong et al., 2006)

involved_in male germ-line cyst encapsulation

inferred from mutant phenotype

(La Marca et al., 2019)

involved_in negative regulation of hippo signaling

inferred from mutant phenotype

(Neal et al., 2022, Pojer et al., 2021, Gil-Ranedo et al., 2019, Zheng et al., 2017, Liu et al., 2016, Ribeiro et al., 2010)

inferred from genetic interaction with FLYBASE:hpo; FB:FBgn0261456

(Neal et al., 2020)

involved_in negative regulation of JNK cascade

inferred from mutant phenotype

(La Marca et al., 2019)

inferred from genetic interaction with FLYBASE:bsk; FB:FBgn0000229

(La Marca et al., 2019)

involved_in positive regulation of JNK cascade

inferred from genetic interaction with FLYBASE:Rac1; FB:FBgn0010333

(Ashton-Beaucage et al., 2014)

inferred from direct assay

(Chen et al., 2002)

inferred from genetic interaction with FLYBASE:bsk; FB:FBgn0000229

(Chen et al., 2002)

inferred from genetic interaction with FLYBASE:Jra; FB:FBgn0001291

(Chen et al., 2002)

inferred from genetic interaction with FLYBASE:kay; FB:FBgn0001297

(Chen et al., 2002)

inferred from genetic interaction with FLYBASE:hep; FB:FBgn0010303

(Chen et al., 2002)

involved_in positive regulation of JUN kinase activity

inferred from mutant phenotype

(Xu et al., 2023)

involved_in positive regulation of neuroblast proliferation

inferred from mutant phenotype

(Gil-Ranedo et al., 2019)

involved_in positive regulation of Ras protein signal transduction

inferred from high throughput genetic interaction with FLYBASE:Ras85D; FB:FBgn0003205

(Ashton-Beaucage et al., 2014)

involved_in R8 cell fate commitment

inferred from mutant phenotype

(Pojer et al., 2021)

involved_in regulation of cellular response to phosphate starvation

inferred from mutant phenotype

(Xu et al., 2023)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in regulation of signal transduction

inferred from biological aspect of ancestor with PANTHER:PTN000402124

(GO Reference Genome Project, 2011-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Chen et al., 2002)

part_of FAR/SIN/STRIPAK complex

inferred from direct assay

(Ashton-Beaucage et al., 2014, Ribeiro et al., 2010)

located_in nucleus

inferred from direct assay

(Chen et al., 2002)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

part_of FAR/SIN/STRIPAK complex

inferred from biological aspect of ancestor with PANTHER:PTN000402124

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

STRIATIN-INTERACTING PHOSPHATASE AND KINASE COMPLEX

Pathway (FlyBase)

NEGATIVE REGULATORS OF HIPPO SIGNALING PATHWAY

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Pathway (FlyBase)

NEGATIVE REGULATORS OF HIPPO SIGNALING PATHWAY -: The Hippo signaling pathway is an intracellular kinase cascade in which hpo kinase in complex with sav, phosphorylates wts kinase which, in turn, phosphorylates yki transcriptional co-activator leading to its cytosolic retention. Negative regulators of the pathway promote the nuclear accumulation of yki, enhancing yki-mediated transcriptional regulation and the expression of genes that positively regulate tissue growth (Adapted from FBrf0224870).

Gene Group (FlyBase)

STRIATIN-INTERACTING PHOSPHATASE AND KINASE COMPLEX -: Striatin interacting phosphatase and kinase (STRIPAK)/STRIPAK-like complexes contain a protein phosphatase type 2A (mts), a striatin (Cka) and other regulatory subunits. Kinases, such as GckIII, are often associated with the complex (FBrf0211636). The STRIPAK complex has a regulatory role in many signaling pathways and cellular processes. (Adapted from FBrf0223805).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Cka for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry D3DML3)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Gene model reviewed during 5.46

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Cka-RA

FBtr0079536

NM_135333

3274

730

Cka-RB

FBtr0079537

NM_164779

5135

730

Cka-RC

FBtr0079539

NM_164780

3254

730

Cka-RD

FBtr0079538

NM_164781

3003

730

Cka-RE

FBtr0306000

NM_001259007

2819

749

Cka-RF

FBtr0306001

NM_001259008

5366

747

Cka-RG

FBtr0333096

NM_001273292

2738

722

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Cka-PA

FBpp0079158

78.0

730

4.86

FBpp0079159

78.0

730

4.86

FBpp0079161

78.0

730

4.86

FBpp0079160

78.0

730

4.86

FBpp0297142

80.1

749

4.68

FBpp0297143

79.9

747

4.71

FBpp0305309

77.1

722

4.71

M9PF42

NP_001260221

AGB92757

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

730 aa isoforms: Cka-PA, Cka-PB, Cka-PC, Cka-PD

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Cka using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Cka_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.74

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism | ubiquitous

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 16

organism | ubiquitous

(Fisher et al., 2012)

third instar larval stage

leg disc | ubiquitous

(Grubbs et al., 2013)

antennal disc | ubiquitous

(Grubbs et al., 2013)

eye disc | ubiquitous

(Grubbs et al., 2013)

wing disc | ubiquitous

(Grubbs et al., 2013)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- larval stage

organism

(Weng and Du, 2002)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Chen et al., 2002)

part_of FAR/SIN/STRIPAK complex

inferred from direct assay

(Ashton-Beaucage et al., 2014, Ribeiro et al., 2010)

located_in nucleus

inferred from direct assay

(Chen et al., 2002)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Cka in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Cka

View exonic expression by tissue for Dmel\Cka

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0044323

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34096/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0044323

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0044323

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0044323

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7392

Flygut - An atlas of the Drosophila adult midgut

FBgn0044323

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 18 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 14 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Cka

Transgenic constructs containing regulatory region of Cka

Aberrations (Deficiencies and Duplications) ( 4 )

Inferred from experimentation ( 4 )

Gene not disrupted in

Df(2L)TE29Aa-11

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Df(2L)XE-2750

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Df(2L)XE-3801

(BDGP Project Members, 1994-1999)

Gene disrupted in

Df(2L)baf²

(Furukawa et al., 2003)

Inferred from location ( 2 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^arm.PS

Cka^HMS00081

lethal (with Cka⁰⁵⁸³⁶)

Cka^{MI13708-TG4.0}

lethal (with Cka^{MI13708-TG4.0})

Cka⁰⁵⁸³⁶

lethal | embryonic stage | maternal effect | recessive

Cka⁰⁵⁸³⁶

lethal | embryonic stage | rescuable maternal effect

Cka⁰⁵⁸³⁶

lethal | pupal stage | recessive

Cka⁰⁵⁸³⁶

lethal | recessive

lethal - all die before end of P-stage, with Scer\GAL4^da.G32

Cka^HMS00081

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

Cka^GD12225

lethal - all die before end of pupal stage (with Cka⁰⁵⁸³⁶)

Cka^s1883

lethal - all die before end of pupal stage (with Cka^s1883)

Cka⁰⁵⁸³⁶

lethal - all die before end of pupal stage | recessive

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

Cka^GD12225

viable

Cka^EY10818

viable, with Scer\GAL4^tin.CΔ4

Cka^GD12225

Sterility

Allele

female fertile, with Scer\GAL4^{VP16.nanos.UTR}

Cka^HMS00081

fertile

Cka^EY10818

male semi-sterile, with Scer\GAL4^{VP16.nanos.UTR}

Cka^HMS00081

Other Phenotypes

Allele

abnormal gravitaxis

Cka^c305a

abnormal neuroanatomy, with Scer\GAL4⁴⁷⁷, Scer\GAL4^ppk.PU

Cka^GD12225

abnormal neuroanatomy | larval stage | somatic clone - tissue specific, with Scer\GAL4^Act5C.PI

Cka^HMS00081

abnormal neuroanatomy | third instar larval stage, with Hsim\VP16^AD.nrv2, Scer\GAL4^{DBD.wrapper.932i}

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^RapGAP1-OK6

Cka^HM05138

abnormal size | male | third instar larval stage, with Scer\GAL4^C587

abnormal size | male | third instar larval stage, with Scer\GAL4^tj.PU

Cka^KK100338

abnormal size | spermatogenesis, with Scer\GAL4^{VP16.nanos.UTR}

Cka^HMS00081

atrophy | pupal stage, with Scer\GAL4^Mef2.PR

increased body size | P-stage, with Scer\GAL4^Mef2.PR

increased size | pupal stage, with Scer\GAL4^Mef2.PR

short lived | heat sensitive, with Scer\GAL4^tin.CΔ4

Cka^GD12225

some die during larval stage, with Scer\GAL4^da.G32

Cka^HMS00081

some die during P-stage, with Scer\GAL4^da.G32

Cka^HMS00081

some die during pupal stage, with Scer\GAL4^pnr-MD237

Cka^GD12225

some die during pupal stage (with Cka⁰⁵⁸³⁶)

Cka^s1883

some die during pupal stage (with Cka^s1883)

Cka⁰⁵⁸³⁶

some die during pupal stage | recessive

visible, with Scer\GAL4^Dll-md23

Cka^HMS00081

Phenotype manifest in

Allele

arista, with Scer\GAL4^Dll-md23

Cka^HMS00081

cyst cell of testis | third instar larval stage, with Scer\GAL4^C587

Cka^GD12225

dendrite, with Scer\GAL4⁴⁷⁷, Scer\GAL4^ppk.PU

Cka^GD12225

dendritic branch | decreased number, with Scer\GAL4⁴⁷⁷, Scer\GAL4^ppk.PU

Cka^GD12225

developing neuron | ectopic | larval stage | somatic clone - tissue specific, with Scer\GAL4^Act5C.PI

Cka^HMS00081

embryonic/first instar larval cuticle

Cka⁰⁵⁸³⁶

embryonic/first instar larval cuticle | dorsal

Cka⁰⁵⁸³⁶

embryonic/first instar larval cuticle | dorsal | germline clone | rescuable maternal effect

Cka⁰⁵⁸³⁶

embryonic/larval cell body glial cell | third instar larval stage, with Hsim\VP16^AD.nrv2, Scer\GAL4^{DBD.wrapper.932i}

embryonic/larval hypodermal muscle | larval stage, with Scer\GAL4^Mef2.PR

embryonic dorsal epidermis | germline clone | maternal effect

embryonic dorsal epidermis | germline clone | rescuable maternal effect

Cka⁰⁵⁸³⁶

embryonic head | germline clone | rescuable maternal effect

Cka⁰⁵⁸³⁶

embryonic leading edge cell | germline clone | maternal effect

Cka⁰⁵⁸³⁶

eye disc | larval stage | somatic clone - tissue specific, with Scer\GAL4^Act5C.PI

Cka^HMS00081

filamentous actin | larval stage, with Scer\GAL4^Mef2.PR

gonad | male | third instar larval stage, with Scer\GAL4^C587

gonad | male | third instar larval stage, with Scer\GAL4^tj.PU

Cka^KK100338

head

Cka⁰⁵⁸³⁶

imaginal disc

Cka^k12603

imaginal disc (with Cka⁰⁵⁸³⁶)

Cka^s1883

imaginal disc (with Cka^s1883)

Cka⁰⁵⁸³⁶

indirect flight muscle cell | pupal stage, with Scer\GAL4^Mef2.PR

investment cone, with Scer\GAL4^{VP16.nanos.UTR}

Cka^HMS00081

male germline cyst | third instar larval stage, with Scer\GAL4^C587

Cka^GD12225

male germline stem cell | adult stage, with Scer\GAL4^Act5C.PU

Cka^HMS00081

male germline stem cell | third instar larval stage, with Scer\GAL4^C587

Cka^GD12225

multidendritic neuron, with Scer\GAL4⁴⁷⁷, Scer\GAL4^ppk.PU

Cka^GD12225

myotube | pupal stage, with Scer\GAL4^Mef2.PR

NMJ bouton | increased number, with Scer\GAL4^RapGAP1-OK6

Cka^HM05138

peripodial epithelium of eye disc | larval stage | somatic clone - tissue specific, with Scer\GAL4^Act5C.PI

Cka^HMS00081

pulvillus, with Scer\GAL4^Dll-md23

Cka^HMS00081

spermatogonium | third instar larval stage, with Scer\GAL4^C587

Cka^GD12225

tarsal segment, with Scer\GAL4^Dll-md23

Cka^HMS00081

terminal epithelium of testis, with Scer\GAL4^{VP16.nanos.UTR}

Cka^HMS00081

testis | adult stage, with Scer\GAL4^Act5C.PU

Cka^HMS00081

third segment of antenna, with Scer\GAL4^Dll-md23

Cka^HMS00081

unguis, with Scer\GAL4^Dll-md23

Cka^HMS00081

ventral longitudinal muscle 3 | larval stage, with Scer\GAL4^Mef2.PR

ventral longitudinal muscle 4 | larval stage, with Scer\GAL4^Mef2.PR

wing, with Scer\GAL4^Dll-md23

Cka^HMS00081

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (19)

Hsap\STRN3

14 of 14

Yes

Hsap\STRN

13 of 14

Yes

Hsap\STRN4

12 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (11)

Rnor\Strn3

13 of 14

Yes

Rnor\Strn

13 of 14

Yes

Rnor\Strn4

12 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (13)

Mmus\Strn3

14 of 14

Yes

Mmus\Strn

13 of 14

Yes

Mmus\Strn4

11 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (10)

Xtro\strn

10 of 13

Yes

Xtro\strn3

6 of 13

Yes

5 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (11)

Drer\strn3

14 of 14

Yes

Drer\strn

11 of 14

Yes

Drer\strn4

9 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (8)

Cele\cash-1

14 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (11)

Agam\AgaP_AGAP008755

7 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (11)

Atha\AT1G48870

1 of 13

Yes

Atha\AT1G62020

1 of 13

Yes

Atha\AT2G40360

1 of 13

Yes

Atha\AT2G46290

1 of 13

Yes

Atha\AT4G04940

1 of 13

Yes

Atha\LIS

1 of 13

Yes

Atha\MAC3A

1 of 13

Yes

Atha\PWP2

1 of 13

Yes

Atha\TAF5

1 of 13

Yes

Atha\WDR5a

1 of 13

Yes

Atha\WDR5b

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (8)

Scer\FAR8

4 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (4)

Spom\SPBC1773.01

9 of 12

Yes

Spom\SPBC4F6.13c

1 of 12

Spom\SPBPJ4664.04

1 of 12

Spom\taf5

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Cka. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (7)

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

wds

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

14 of 14

13 of 14

12 of 14

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Cka

anti tag coimmunoprecipitation, anti tag western blot

(Neisch et al., 2017, Ashton-Beaucage et al., 2014)

Cka - Atg8a

pull down, western blot

(Neisch et al., 2017)

Cka - Clk

anti bait coimmunoprecipitation, anti tag western blot

(Andreazza et al., 2015)

Cka - Dhc64C

anti tag coimmunoprecipitation, western blot

(Neisch et al., 2017)

Cka - Dlg5

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Kaya-Çopur et al., 2021)

Cka - Fgop2

anti tag coimmunoprecipitation, anti tag western blot, peptide massfingerprinting

(Ashton-Beaucage et al., 2014, Ribeiro et al., 2010)

Cka - GckIII

anti tag coimmunoprecipitation, peptide massfingerprinting

(Ribeiro et al., 2010)

Cka - Jra

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation

(Chen et al., 2002)

Cka - Mob4

anti tag coimmunoprecipitation, peptide massfingerprinting, two hybrid, anti tag western blot

(Hu et al., 2017.6.13, Ashton-Beaucage et al., 2014, Ribeiro et al., 2010)

Cka - Naus

anti tag coimmunoprecipitation, peptide massfingerprinting

(Ribeiro et al., 2010)

Cka - PXo

anti tag coimmunoprecipitation, anti tag western blot, Identification by mass spectrometry

(Xu et al., 2023)

Cka - Pp2A-29B

anti tag coimmunoprecipitation, anti tag western blot, peptide massfingerprinting

(Neisch et al., 2017, Ribeiro et al., 2010)

Cka - Rassf

anti tag coimmunoprecipitation, peptide massfingerprinting, anti tag western blot

(Shen et al., 2022, Ribeiro et al., 2010)

Cka - Slik

anti tag coimmunoprecipitation, anti tag western blot

(De Jamblinne et al., 2020)

Cka - Slmap

anti tag coimmunoprecipitation, anti tag western blot

(Ashton-Beaucage et al., 2014)

Cka - Strip

anti tag coimmunoprecipitation, anti tag western blot, anti bait coimmunoprecipitation, western blot, peptide massfingerprinting

(La Marca et al., 2019, Andreazza et al., 2015, Ashton-Beaucage et al., 2014, Ribeiro et al., 2010)

Cka - Vta1

two hybrid

(Hu et al., 2017.6.13)

Cka - bsk

anti tag coimmunoprecipitation, western blot

(Chen et al., 2002)

Cka - hep

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation

(La Marca et al., 2019, Chen et al., 2002)

Cka - hpo

anti tag coimmunoprecipitation, peptide massfingerprinting, anti tag western blot, western blot

(Zheng et al., 2017, Ribeiro et al., 2010)

Cka - kay

anti tag coimmunoprecipitation, western blot, anti bait coimmunoprecipitation

(Chen et al., 2002)

Cka - msn

anti tag coimmunoprecipitation, peptide massfingerprinting

(Ribeiro et al., 2010)

Cka - mts

anti tag coimmunoprecipitation, peptide massfingerprinting

(Ribeiro et al., 2010)

Cka - pll

anti tag coimmunoprecipitation, anti tag western blot, enzymatic study

(Liu et al., 2016)

Cka - sw

anti tag coimmunoprecipitation, western blot

(Neisch et al., 2017)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Cka

suppressible

bsk

(La Marca et al., 2019)

Cka

suppressible

hep

(Chen et al., 2002)

Cka

suppressible

Jra

(Chen et al., 2002)

enhanceable

(Guo et al., 2023)

enhanceable

(Guo et al., 2023)

suppressible

(Chen et al., 2002)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

bsk

enhanceable

Cka

(Chen et al., 2002)

hep

suppressible

Cka

(Chen et al., 2002)

kay

enhanceable

Cka

(Chen et al., 2002)

Mitf

suppressible

Cka

(Zhang et al., 2024)

suppressible

(Xu et al., 2023)

suppressible

(Ashton-Beaucage et al., 2014)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

60230

DroID - A comprehensive database of gene and protein interactions.

FBgn0044323

MIST (protein-protein) - An integrated Molecular Interaction Database

34096

Pathways

Signaling Pathways (FlyBase)

NEGATIVE REGULATORS OF HIPPO SIGNALING PATHWAY

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

MAPK signaling pathway - fly

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q86M30

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-29

Cytogenetic map

28D3-28D3

Sequence location

2L:8,030,721..8,040,826 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

28D3-28D3

Limits computationally determined from genome sequence between P{lacW}l(2)k10210^k10210 and P{EP}poe^EP737

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

28E-28E

(Hou, 2001.3.13)

28E1-28E2

(determined by in situ hybridisation)

(Spradling et al., 1999, Perrimon et al., 1996)

28D8-28D11

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

2-29

(FlyBase, 2016)

2-26.4

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (21)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

53756

w^*; P{UASp-Cka.EGFP.C}2

30829

w^*; P{GawB}Cka^c305a

12039

w¹¹¹⁸; P{lacW}Cka^s1883/CyO

28927

y¹ v¹; P{TRiP.HM05138}attP2

31642

y¹ v¹; P{TRiP.JF01432}attP2

53757

w^*; P{UASp-Cka.EGFP.C}3/TM3, Sb¹

11451

P{PZ}Cka⁰⁵⁸³⁶ cn¹/CyO; ry⁵⁰⁶

Kyoto

102839

y¹ w^67c23 ; P{lacW}Cka^k12603 / CyO

102840

y¹ w^67c23 ; P{lacW}Cka^k12606 / CyO

NIG-Fly

M2L-0510

y¹ w¹¹¹⁸; Cka^SK1 P{neoFRT}40A/CyO-Tb

VDRC

v106971

P{KK100338}VIE-260B

v35234

w¹¹¹⁸; P{GD12225}v35234

More stocks available...

Genomic Clones (10)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (313)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD41760

Other clones

MIP15828

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0044323

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0044323

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Weng and Du, 2002)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.

(Stroschein-Stevenson et al., 2006)

dsRNA made from templates generated with primers directed against Cka that is transfected into S2 treated with Listeria monocytogenes reveals Cka to be involved in Listeria monocytogenes entry.

(Cheng et al., 2005)

Cka controls the localized expression of dpp in the leading edge cells.

(Chen et al., 2001)

Cka functions in the JNK dorsal closure pathway.

(Chen et al., 2001)

Identification: Enhancer trap expression pattern survey for loci expressed in the ring gland.

(Harvie et al., 1998)

Mutants isolated in a screen of the second chromosome identifying genes affecting disc morphology.

(Roch et al., 1998)

The autosomal "FLP-DFS" technique (using the P{ovoD1-18} P{FRT(w^hs)} P{hsFLP} chromosomes) has been used to identify the specific maternal effect phenotype for the zygotic lethal mutation.

(Perrimon et al., 1996)

Relationship to Other Genes

Source for database merge of

Source for merge of: WD-40-family-member CG7392

(Bayraktaroglu, 2000.4.12)

Source for merge of: WD-40-family-member l(2)05836

(Misra, 2000.11.18)

Source for merge of: Cka WD-40-family-member

(FlyBase, 1996-)

Source for merge of: Cka anon-WO0172774.174 anon-WO0172774.175 anon-WO0172774.177 anon-WO0172774.179

(FlyBase, 1996-)

Additional comments

Source for merge of Cka WD-40-family-member was sequence comparison ( date:020313 ).

(FlyBase, 1996-)

Source for merge of Cka anon-WO0172774.174 anon-WO0172774.175 anon-WO0172774.177 anon-WO0172774.179 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

The gene is named "connector of kinase to AP-1" based on its presumed role in the JNK signal transduction pathway.

(Chen et al., 2002)

Synonyms and Secondary IDs (17)

Reported As

Symbol Synonym

5836

(Harvie et al., 1998)

CG7392

(National Institute of Genetics Fly Stocks, 2022-, Hosono et al., 2015, Ni et al., 2011.7.19, Müller et al., 2010, Keleman et al., 2009.8.5, Perkins et al., 2009, Ni et al., 2008, Konduri and Beckingham, 2007, Armstrong et al., 2006, Konduri and Beckingham, 2006, Anholt and Mackay, 2001, Mount, 2001.8.14, Melnick, 1997.6.26)

CKA

(Gohel et al., 2020, Yang et al., 2003, Weng and Du, 2002, Chen et al., 2001)

Cka

(Meyer et al., 2024, Xu et al., 2023, Ding et al., 2022, National Institute of Genetics Fly Stocks, 2022-, Perlegos et al., 2022, Shen et al., 2022, Herrera and Bach, 2021, Huang et al., 2021, Pojer et al., 2021, De Jamblinne et al., 2020, Ding et al., 2020, Huang et al., 2020, La Marca and Richardson, 2020, Neal et al., 2020, La Marca et al., 2019, Lee et al., 2018, Ashton-Beaucage and Therrien, 2017, Hu et al., 2017.6.13, Neisch et al., 2017, Transgenic RNAi Project members, 2017-, Zheng et al., 2017, Clandinin and Owens, 2016-, Gene Disruption Project members, 2016-, Liu et al., 2016, Liu et al., 2016, Sakuma et al., 2016, van der Harst et al., 2016, Andreazza et al., 2015, Madsen et al., 2015, Ríos-Barrera et al., 2015, Thompson and Sahai, 2015, Ashton-Beaucage et al., 2014, Hwang and Pallas, 2014, Sakuma et al., 2014, Carter, 2013, Grubbs et al., 2013, Ríos-Barrera and Riesgo-Escovar, 2013, Murray et al., 2012, Friedman et al., 2011, Guest et al., 2011, Herranz et al., 2010, Müller et al., 2010, Müller et al., 2010, Bond and Foley, 2009, Zhou et al., 2008, Armstrong et al., 2006, Stroschein-Stevenson et al., 2006, Furukawa et al., 2003)

Dcka

(Oh, 2001.3.26)

anon-WO0172774.174

anon-WO0172774.175

anon-WO0172774.177

anon-WO0172774.179

cka

(Huang et al., 2020, Sharma et al., 2020, Zheng et al., 2017, Chen et al., 2015, Williams et al., 2014, Ribeiro et al., 2010, Zhang et al., 2010, Chen et al., 2002)

l(2)05836

(Spradling et al., 1999, Roch et al., 1998, Russell et al., 1998, Perrimon et al., 1996, BDGP Project Members, 1994-1999)

l(2)s1883

Name Synonyms

AP-1

(Coutinho-Budd et al., 2017)

Connector of kinase to AP-1

(Andreazza et al., 2015, Ribeiro et al., 2010, Armstrong et al., 2006, Chen et al., 2001)

WD-40-family-member

(Misra, 2000.11.18)

connector of kinase to

(Coutinho-Budd et al., 2017)

connector of kinase to AP-1

(Chen et al., 2002)

Secondary FlyBase IDs

FBgn0010608
FBgn0010681
FBgn0026012
FBgn0031978
FBgn0046423
FBgn0046425
FBgn0046426
FBgn0046427

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 77 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34096

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

D3DML3

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

D3DML3

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0044323

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34096/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0044323

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0044323

FlyMine - An integrated database for Drosophila genomics

FBgn0044323

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG7392

MARRVEL_MODEL - MARRVEL (model organism gene)

34096

Linkouts

BioGRID - A database of protein and genetic interactions.

60230

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0044323

DroID - A comprehensive database of gene and protein interactions.

FBgn0044323

DRSC - Results frm RNAi screens

FBgn0044323

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Cka_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0044323

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0044323

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7392

Flygut - An atlas of the Drosophila adult midgut

FBgn0044323

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0044323

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC007565

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

MAPK signaling pathway - fly

MIST (protein-protein) - An integrated Molecular Interaction Database

34096

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q86M30

References (139)

The following external sites may use different assemblies or annotations than FlyBase.

0.74

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