FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000086274
Interaction Network
Interactions Browser links
Pp2A-29B network
Cka network
Reported Interactions
FBrf0211636-20.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cka 

Cka

bait

FLAG tag

Pp2A-29B

prey

16 unique peptides identified

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Peptides for this prey protein have been found also in control AP-MS experiments, but were enriched in Cka and Mob4 purifications.

FBrf0235687-6.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Pp2A-29B

bait

FLAG tag

Cka 

Cka

prey

GFP tag

Cka 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cka 
PP2A domain
mutation decreasing interaction
aa 150,151

R150S,R151S coordinates relative to Cka-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)