FlyBase Gene Report: Dmel\Taf5

General Information

Symbol

Dmel\Taf5

Species

D. melanogaster

Name

TBP-associated factor 5

Annotation Symbol

CG7704

Feature Type

protein_coding_gene

FlyBase ID

FBgn0010356

Gene Model Status

Current

Stock Availability

12 publicly available

Gene Summary

TBP-associated factor 5 (Taf5) encodes a protein that forms part of the multisubunit basal transcription factor TFIID and it may be important for its assembly or stability. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

dTAF_II80, Taf80, TAF_II80, TFIID, TAFII80

Key Links

Genomic Location

Cytogenetic map

47C5-47C5

Sequence location

2R:10,877,312..10,879,673 [-]

Recombination map

2-63

RefSeq locus

NT_033778 REGION:10877312..10879673

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (4 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

contributes_to RNA polymerase II general transcription initiation factor activity

inferred from direct assay

(Wright et al., 2006, Hansen and Tjian, 1995)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

contributes_to RNA polymerase II general transcription initiation factor activity

inferred from biological aspect of ancestor with PANTHER:PTN000459161

(GO Reference Genome Project, 2011-)

Biological Process (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in transcription initiation at RNA polymerase II promoter

inferred from direct assay

(Wright et al., 2006)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in transcription initiation at RNA polymerase II promoter

inferred from biological aspect of ancestor with PANTHER:PTN000459161

(GO Reference Genome Project, 2011-)

inferred from sequence or structural similarity with SGD:S000000402

(Aoyagi and Wassarman, 2000)

inferred from sequence or structural similarity with UniProtKB:O75529

(Aoyagi and Wassarman, 2000)

inferred from sequence or structural similarity with UniProtKB:Q15542

(Aoyagi and Wassarman, 2000)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in nucleus

inferred from direct assay

(Wright et al., 2006, Hansen and Tjian, 1995)

part_of transcription factor TFIID complex

inferred from physical interaction with FLYBASE:Taf4; FB:FBgn0010280

(Wright et al., 2006)

inferred from physical interaction with FLYBASE:Taf6; FB:FBgn0010417

(Wright et al., 2006)

inferred from direct assay

(Hansen and Tjian, 1995)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

part_of transcription factor TFIID complex

inferred from sequence or structural similarity with SGD:S000000402

(Aoyagi and Wassarman, 2000)

inferred from sequence or structural similarity with UniProtKB:Q15542

(Aoyagi and Wassarman, 2000)

inferred from biological aspect of ancestor with PANTHER:PTN000459161

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

TRANSCRIPTION FACTOR II D

Protein Family (UniProt)

Belongs to the WD repeat TAF5 family. (P49846)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

TRANSCRIPTION FACTOR II D -: The general transcription factor complex Transcription factor II D (TFIID) plays an important role in recruiting the transcription machinery to core promoters. TFIID is a complex composed of TBP and several TBP-associated factors. (Adapted from FBrf0125116 and FBrf0156065).

Protein Function (UniProtKB)

TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. May play a role in helping to anchor Taf4 within the TFIID complex. May be involved in transducing signals from various transcriptional regulators to the RNA polymerase II transcription machinery.

(UniProt, P49846)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Taf5 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P49846)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.49

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Taf5-RA

FBtr0088240

NM_057609

2362

704

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Taf5-PA

FBpp0087335

79.3

704

6.34

P49846

NP_476957

AAF58737

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

704 (aa); 80 (kD)

(Dynlacht et al., 1993)

704 (aa); 85 (kD observed); 79 (kD predicted)

(Kokubo et al., 1993)

80 (kD)

(Dynlacht et al., 1991)

Comments

External Data

Subunit Structure (UniProtKB)

Belongs to the TFIID complex which is composed of TATA binding protein (Tbp) and a number of TBP-associated factors (Tafs). Interacts with Tbp and Taf4.

(UniProt, P49846)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Taf5 using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.67

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | ubiquitous

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | male

testis

(Metcalf and Wassarman, 2007)

male accessory gland

(Metcalf and Wassarman, 2007)

seminal vesicle

(Metcalf and Wassarman, 2007)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in nucleus

inferred from direct assay

(Wright et al., 2006, Hansen and Tjian, 1995)

part_of transcription factor TFIID complex

inferred from physical interaction with FLYBASE:Taf4; FB:FBgn0010280

(Wright et al., 2006)

inferred from physical interaction with FLYBASE:Taf6; FB:FBgn0010417

(Wright et al., 2006)

inferred from direct assay

(Hansen and Tjian, 1995)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Taf5 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Taf5

View exonic expression by tissue for Dmel\Taf5

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0010356

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

47900/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0010356

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0010356

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0010356

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7704

Flygut - An atlas of the Drosophila adult midgut

FBgn0010356

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 2 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 7 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Taf5

Transgenic constructs containing regulatory region of Taf5

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(2R)Exel6059

(Ryder, 2004.4.26)

Inferred from location ( 10 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Taf5^GD4096

lethal, with Scer\GAL4^insc-Mz1407

Taf5^GD4096

lethal | pupal stage, with Scer\GAL4^pnr-MD237

Taf5^GD4096

lethal - all die before end of P-stage, with Scer\GAL4^phtm.PU

Taf5^GD4096

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

Taf5^GD4096

lethal - all die during pupal stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Taf5^GD4096

partially lethal - majority die | pupal stage, with Scer\GAL4^pnr-MD237

Taf5^GD4096

viable, with Scer\GAL4^tin.CΔ4

Taf5^GD4096

Other Phenotypes

Allele

abnormal neuroanatomy | larval stage, with Scer\GAL4^insc-Mz1407

Taf5^GD4096

short lived | heat sensitive, with Scer\GAL4^tin.CΔ4

Taf5^GD4096

visible, with Scer\GAL4^pnr-MD237

Taf5^GD4096

Phenotype manifest in

Allele

embryonic/larval fat body | wandering third instar larval stage, with Scer\GAL4^ppl.PP

Taf5^GD4096

ganglion mother cell | larval stage, with Scer\GAL4^insc-Mz1407

Taf5^GD4096

larval neuroblast | larval stage, with Scer\GAL4^insc-Mz1407

Taf5^GD4096

lipid droplet | wandering third instar larval stage, with Scer\GAL4^ppl.PP

Taf5^GD4096

mesonotum, with Scer\GAL4^pnr-MD237

Taf5^GD4096

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (61)

Hsap\TAF5

14 of 14

Yes

5 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\WDR70

1 of 14

Hsap\WDSUB1

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (42)

Rnor\Taf5

14 of 14

Yes

5 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Wdr86

1 of 14

Rnor\Wdr88

1 of 14

Yes

Rnor\Wdsub1

1 of 14

Mus musculus (laboratory mouse) (39)

Mmus\Taf5

14 of 14

Yes

Mmus\Taf5l

4 of 14

Mmus\Wdr38

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Wdr88

1 of 14

Yes

Xenopus tropicalis (Western clawed frog) (42)

Xtro\taf5

11 of 13

Yes

Xtro\taf5l

4 of 13

Yes

Xtro\ahi1

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\LOC100491248

1 of 13

Yes

Xtro\LOC101734139

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\strn

1 of 13

Xtro\tep1

1 of 13

Xtro\thul16

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (46)

Drer\taf5

14 of 14

Yes

Drer\taf5l

4 of 14

Drer\ahi1

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\wdr59

1 of 14

Drer\wsb1

1 of 14

Yes

Drer\zmp:0000000529

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (35)

Cele\taf-5

14 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (40)

Agam\AgaP_AGAP011678

12 of 12

Yes

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (56)

Atha\TAF5

12 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (19)

Scer\TAF5

13 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (22)

Spom\taf5

12 of 12

Yes

Spom\taf73

10 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Taf5. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (39)

can

9 of 13

wda

4 of 13

ago

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Cka

1 of 13

ebi

1 of 13

esc

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Nle

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

wds

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

TAF5; TATA-box binding protein associated factor 5

14 of 14

601787

TAF5L; TATA-box binding protein associated factor 5 like

5 of 14

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Taf5 - Ada2b

anti bait coimmunoprecipitation, western blot

(Kusch et al., 2003)

Taf5 - Ada3

anti bait coimmunoprecipitation, western blot

(Kusch et al., 2003)

Taf5 - Bap170

coimmunoprecipitation, western blot

(Vorobyeva et al., 2009)

Taf5 - CG1532

experimental knowledge based

(Guruharsha et al., 2011)

Taf5 - CG18787

experimental knowledge based

(Guruharsha et al., 2011)

Taf5 - CG4291

experimental knowledge based

(Guruharsha et al., 2011)

Taf5 - CG7049

experimental knowledge based

(Guruharsha et al., 2011)

Taf5 - Pc

anti bait coimmunoprecipitation, western blot

(Saurin et al., 2001)

Taf5 - Psc

anti bait coimmunoprecipitation, western blot

(Saurin et al., 2001)

Taf5 - Rae1

experimental knowledge based

(Guruharsha et al., 2011)

Taf5 - Sod1

experimental knowledge based

(Guruharsha et al., 2011)

Taf5 - Spt3

anti bait coimmunoprecipitation, western blot

(Kusch et al., 2003)

Taf5 - Taf1

affinity chromatography technology, western blot, anti bait coimmunoprecipitation

(Vorobyeva et al., 2009, Santoso and Kadonaga, 2006)

Taf5 - Taf12

anti bait coimmunoprecipitation, western blot

(Yokomori et al., 1993)

Taf5 - Taf2

anti bait coimmunoprecipitation, western blot

(Verrijzer et al., 1994)

Taf5 - Taf4

anti bait coimmunoprecipitation, western blot

(Pahi et al., 2015, Wright et al., 2006, Saurin et al., 2001)

Taf5 - Taf6

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2009, Wright et al., 2006)

Taf5 - Taf8

anti bait coimmunoprecipitation, western blot

(Vorobyeva et al., 2009)

Taf5 - Taf9

anti bait coimmunoprecipitation, western blot

(Saurin et al., 2001)

Taf5 - Tbp

anti bait coimmunoprecipitation, western blot, molecular weight estimation by staining

(Saurin et al., 2001, Chen et al., 1994, Verrijzer et al., 1994, Yokomori et al., 1993)

Taf5 - e(y)3

anti bait coimmunoprecipitation, Identification by mass spectrometry, coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, anti tag western blot

(Vorobyeva et al., 2009)

Taf5 - mor

anti bait coimmunoprecipitation, western blot

(Vorobyeva et al., 2009)

Taf5 - mtSSB

experimental knowledge based

(Guruharsha et al., 2011)

Taf5 - ph-p

anti tag coimmunoprecipitation, ion exchange chromatography, molecular sieving, western blot, anti bait coimmunoprecipitation, peptide massfingerprinting

(Saurin et al., 2001)

Taf5 - polybromo

anti bait coimmunoprecipitation, western blot

(Vorobyeva et al., 2009)

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Subunit Structure (UniProtKB)

Belongs to the TFIID complex which is composed of TATA binding protein (Tbp) and a number of TBP-associated factors (Tafs). Interacts with Tbp and Taf4.

(UniProt, P49846 )

Linkouts

BioGRID - A database of protein and genetic interactions.

71045

DroID - A comprehensive database of gene and protein interactions.

FBgn0010356

MIST (protein-protein) - An integrated Molecular Interaction Database

47900

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Basal transcription factors

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

P49846

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-63

Cytogenetic map

47C5-47C5

Sequence location

2R:10,877,312..10,879,673 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

47C5-47C5

Limits computationally determined from genome sequence between P{lacW}l(2)k15826^k15826&P{EP}EP471 and P{EP}shn^EP2359&P{EP}shn^EP644

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

47C5-47C6

(determined by in situ hybridisation)

(Dynlacht et al., 1993)

Experimentally Determined Recombination Data

Location

2-63

(FlyBase, 2016)

2-62.3

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (12)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

80568

w^*; P{UAS-Dam-Taf5}attP2

26909

y¹ w^*; P{EP}Taf5^G2965/CyO

15373

y¹ w^67c23; P{EPgy2}EY01764a P{EPgy2}Taf5^EY01764b/CyO

60101

y¹ sc^* v¹ sev²¹; P{TRiP.HMC05095}attP40/CyO

35367

y¹ sc^* v¹ sev²¹; P{TRiP.GL00280}attP2/TM3, Sb¹

FlyORF

F001565

M{UAS-Taf5.ORF}ZH-86Fb

F000817

M{UAS-Taf5.ORF.3xHA}ZH-86Fb

Kyoto

114413

y^d2 w¹¹¹⁸ P{ey-FLP.N}2; P{neoFRT}42D P{EPgy2}EY01764a P{EPgy2}Taf5^EY01764b /CyO y⁺

NIG-Fly

2RG-0326

y² VhaAC39-1^cho-1 v¹; P{U6-Taf5.NIG.sgRNA}attP40/CyO

GL00280

y¹ sc^* v¹ sev²¹; P{TRiP.GL00280}attP2/TM3, Sb¹

VDRC

v45955

w¹¹¹⁸; P{GD4096}v45955

v45957

w¹¹¹⁸; P{GD4096}v45957

Genomic Clones (15)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (23)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD42828

Other clones

DPiM_LD42828

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0010356

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0010356

Antibody Information

Laboratory Generated Antibodies

monoclonal

(Dynlacht et al., 1993)

polyclonal

(Kokubo et al., 1993)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

dsRNA has been made from templates generated with primers directed against this gene.

(Marr et al., 2006)

The proteins encoded by Taf4, Taf5, Taf6, e(y)1 and Taf12 form a core sub-complex of TFIID.

(Wright et al., 2006)

Does not interact, in vitro, with human thryroid-hormone receptor-β.

(Petty et al., 1996)

The assembly and purification of various functional Tbp TAF complexes demonstrate that Tbp plus the TAFs is sufficient to replace endogenous TFIID to support activated transcription in vitro.

(Chen et al., 1994)

TFIID subunit proteins and the Tbp protein can interact in pairwise combinations with several subunits in a network of interactions within TFIID.

(Kokubo et al., 1994)

The TFIID complex interacts with the promoters of Hsp70Bb, Hsp70Bc, Hsp26 and His3 making contacts at the TATA element, initiator, +18 and +28 regions.

(Purnell et al., 1994)

Purified TAF80 is unable to bind TBP directly or to interact strongly with the C-terminal domain of TAF250, but can only interact with a complex containing TBP, TAF250, TAF110 and TAF60.

(Dynlacht et al., 1993)

A number of polypeptides (encoded by Taf1, Taf4, Taf5, Taf6, e(y)1 and Taf12) that are tightly associated with Tbp and are native TFIID components have been purified. Protein blotting experiments suggest that one of these proteins, Taf1, interacts directly with Tbp, while the association between Tbp and the other proteins is either weak or is an indirect association via Taf1.

(Kokubo et al., 1993)

Taf5 cDNA is isolated, the carboxy-terminal region contains WD-40 repeats, and the interactions of Taf5 with other TFIID subunits and initial structure-function relationships relating to the WD-40 repeats are studied.

(Kokubo et al., 1993)

Relationship to Other Genes

Source for database merge of

Source for merge of: Taf80 CG7704

(Bayraktaroglu, 2000.4.17)

Additional comments

The Taf5 gene may have been derived from the wda gene by retroposition.

(Pan and Zhang, 2009)

"Taf80" may correspond to "Scim24". Sequence analysis off ends of P{SUPor-P} in Scim insertion mutant places "Scim24" near/in "CG6751" or "Taf80".

(Dobie and Karpen, 2001.4.18)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (32)

Reported As

Symbol Synonym

CG7704

(National Institute of Genetics Fly Stocks, 2022-, Fan et al., 2017, Çiçek et al., 2016, Schertel et al., 2013, Ni et al., 2011.7.19, Pan and Zhang, 2009, Perkins et al., 2009, Dobie and Karpen, 2001.4.18, Aoyagi and Wassarman, 2000)

TAF

(Müller and Tora, 2004)

TAF5

(Neves and Eisenman, 2019, Xie et al., 2014, Lee et al., 2010, Vorobyeva et al., 2009, Vorobyeva et al., 2009, Weake et al., 2009, Wright and Tjian, 2009, Soshnikova et al., 2008, Isogai et al., 2007, Metcalf and Wassarman, 2007, Marr et al., 2006, Santoso and Kadonaga, 2006, Wright et al., 2006, Tora, 2002)

TAF80

(Hansen et al., 1997)

TAF80/85

(Green, 2000)

TAF_II

(Arnosti, 2002)

TAF_II80

(Stathopoulos et al., 2002, Tora, 2002, Verrijzer, 2001, Wassarman and Sauer, 2001, Adams et al., 2000, Hansen and Tjian, 1995)

TAF_II80/85

(Shen et al., 1998, Tansey and Herr, 1997)

TAF_II85

(Wang and Brock, 2003)

TAF^II80

(Lebedeva, 2005, Lebedeva et al., 2005)

TAFII80

(Bassett et al., 1997, Dynlacht et al., 1993, Dynlacht et al., 1991)

TAFII85

(Schwartz and Pirrotta, 2007)

TFIID

(Butler and Kadonaga, 2002, Gu et al., 2002, Hochheimer et al., 2002, Stathopoulos and Levine, 2002, Mizuguchi et al., 2001)

TFIID 85

(Nakatani, 1994.2.8)

Taf5

(Okuda et al., 2022, Sureka and Mishra, 2021, Lindström et al., 2017, Shih et al., 2016, Pahi et al., 2015, dos Santos, 2014, Schertel et al., 2013, Spedale et al., 2012, Tokusumi et al., 2012, Friedman et al., 2011, Zhao et al., 2008)

Taf80

(Egger et al., 2002, Kal et al., 2000, Bell and Tora, 1999, Jimenez et al., 1999, Struhl and Moqtaderi, 1998, Verrijzer et al., 1995, Hori and Carey, 1994, Purnell et al., 1994, Hoey et al., 1993)

Taf85

Taf_II80

(Chen et al., 1994)

d80

(Pugh, 2000)

dTAF5

(Guelman et al., 2006)

dTAF_II80

(Wright et al., 2006, Gangloff et al., 2001, Hiller et al., 2001, Veenstra and Wolffe, 2001, Albright and Tjian, 2000, Georgieva et al., 2000, Fuller et al., 1999, Zhou et al., 1998, Chen and Tjian, 1996, Damania and Alwine, 1996, Goodrich and Tjian, 1994, Verrijzer et al., 1994, Yokomori et al., 1993, Yokomori et al., 1993)

dTAFII85

(Saurin et al., 2001)

dTAFii80

(Tata and Hartley, 1993)

dmTAF5

(Lebedeva, 2005, Lebedeva et al., 2005)

dmTaf5

(Kusch et al., 2003)

p85

(Kokubo et al., 1994, Kokubo et al., 1993, Kokubo et al., 1993)

taf5

(Fan et al., 2017)

Name Synonyms

TBP associated factor 80

(Dynlacht et al., 1991)

TBP-associated factor

(Schuettengruber et al., 2007)

TBP-associated factor 5

TBP-associated factor 80

(Dynlacht et al., 1993)

TBP-associated factor 80kD

Secondary FlyBase IDs

FBgn0033563

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 50 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

47900

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P49846

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P49846

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P49846

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0010356

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

47900/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0010356

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0010356

FlyMine - An integrated database for Drosophila genomics

FBgn0010356

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG7704

MARRVEL_MODEL - MARRVEL (model organism gene)

47900

Linkouts

BioGRID - A database of protein and genetic interactions.

71045

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0010356

DroID - A comprehensive database of gene and protein interactions.

FBgn0010356

DRSC - Results frm RNAi screens

FBgn0010356

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0010356

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0010356

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7704

Flygut - An atlas of the Drosophila adult midgut

FBgn0010356

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0010356

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC013143

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Basal transcription factors

MIST (protein-protein) - An integrated Molecular Interaction Database

47900

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

P49846

References (121)

The following external sites may use different assemblies or annotations than FlyBase.

-0.67

Report Sections