FlyBase Gene Report: Dmel\CG34133

General Information

Symbol

Dmel\CG34133

Species

D. melanogaster

Name

Annotation Symbol

CG34133

Feature Type

protein_coding_gene

FlyBase ID

FBgn0083969

Gene Model Status

Current

Stock Availability

6 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

99C5-99C5

Sequence location

3R:29,869,365..29,875,159 [+]

Recombination map

3-99

RefSeq locus

NT_033777 REGION:29869365..29875159

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (5 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables molecular sequestering activity

inferred from biological aspect of ancestor with PANTHER:PTN001022429

(GO Reference Genome Project, 2011-)

Biological Process (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in cytosolic ciliogenesis

inferred from biological aspect of ancestor with PANTHER:PTN001022429

(GO Reference Genome Project, 2011-)

involved_in regulation of vesicle-mediated transport

inferred from biological aspect of ancestor with PANTHER:PTN001022429

(GO Reference Genome Project, 2011-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in endosome membrane

inferred from biological aspect of ancestor with PANTHER:PTN001022429

(GO Reference Genome Project, 2011-)

is_active_in Golgi apparatus

inferred from biological aspect of ancestor with PANTHER:PTN001022429

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\CG34133 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q8IMK1)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Conservation across Drosophila species: merge triggered by cross-species new exon data.

Gene model reviewed during 5.46

Gene model reviewed during 5.47

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

CG34133-RB

FBtr0110888

NM_001043308

3831

1078

CG34133-RC

FBtr0301073

NM_001043307

4263

1108

CG34133-RE

FBtr0335004

NM_143485

4209

1090

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

CG34133-PB

FBpp0110188

117.7

1078

8.14

FBpp0290295

121.2

1108

7.81

FBpp0307012

119.1

1090

7.92

A0A0C4DHB8

NP_651742

AAF56961

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CG34133 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG34133_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

1.67

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 16

organism | ubiquitous

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\CG34133 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\CG34133

View exonic expression by tissue for Dmel\CG34133

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0083969

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

318566/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0083969

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0083969

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0083969

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7814

Flygut - An atlas of the Drosophila adult midgut

FBgn0083969

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 4 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of CG34133

Transgenic constructs containing regulatory region of CG34133

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 4 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

CG34133^GD12292

viable

CG34133^c02849

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

viable, with Scer\GAL4^Mef2.PR

viable, with Scer\GAL4^pnr-MD237

CG34133^GD13360

viable, with Scer\GAL4^tin.CΔ4

CG34133^GD12292

Sterility

Allele

fertile

CG34133^c02849

Other Phenotypes

Allele

some die during pupal stage, with Scer\GAL4^pnr-MD237

CG34133^GD12292

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (37)

Hsap\WDR44

12 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (14)

Rnor\Wdr44

11 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (15)

Mmus\Wdr44

12 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (27)

Xtro\wdr44

6 of 13

Yes

Xtro\ahi1

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Danio rerio (Zebrafish) (15)

Drer\wdr44

12 of 14

Yes

Drer\ahi1

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (13)

Cele\sym-4

11 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (27)

Agam\AgaP_AGAP000142

12 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (38)

Atha\AT3G15470

11 of 13

Yes

Atha\AT5G54200

11 of 13

Yes

Atha\AT1G48870

9 of 13

Yes

Atha\AT2G37670

9 of 13

Yes

Atha\AT5G24320

9 of 13

Yes

Atha\AT1G64610

8 of 13

Yes

Atha\AT5G02430

8 of 13

Yes

Atha\AT5G42010

8 of 13

Yes

Atha\AT5G53500

8 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\AT5G23430

1 of 13

Atha\AT5G50230

1 of 13

Atha\AT5G50970

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (17)

Scer\DGR2

9 of 13

Yes

Scer\LAF1

9 of 13

Yes

Scer\TUP1

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (9)

Spom\SPAC3H5.08c

9 of 12

Yes

Spom\SPBC18H10.05

4 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:CG34133. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (31)

ago

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

ebi

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

wds

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

WDR44; WD repeat domain 44

12 of 14

301070

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

CG34133 - Cth

experimental knowledge based

(Guruharsha et al., 2011)

CG34133 - Rab11

pull down, peptide massfingerprinting

(Gillingham et al., 2014)

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

76884

DroID - A comprehensive database of gene and protein interactions.

FBgn0083969

MIST (protein-protein) - An integrated Molecular Interaction Database

318566

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q0KHZ4

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-99

Cytogenetic map

99C5-99C5

Sequence location

3R:29,869,365..29,875,159 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

99C5-99C5

Limits computationally determined from genome sequence between P{PZ}ncd⁰⁵⁸⁸⁴ and P{lacW}l(3)s2500^s2500

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-99

(FlyBase, 2016)

3-97.1

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (6)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

11057

w¹¹¹⁸; PBac{PB}CG34133^c02849

79828

y¹ sc^* v¹ sev²¹; P{TOE.GS02379}attP40

Kyoto

104677

y^* w^*; P{GawB}CG34133^NP4435 / TM6, P{UAS-lacZ.UW23-1}UW23-1

31035R-3

v105062

v35280

w¹¹¹⁸; P{GD12292}v35280

Genomic Clones (15)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (50)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

GH19431

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0083969

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0083969

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

New annotation (CG31035) in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Area matching Drosophila EST AA948996. This EST forms 781bp EST contig with AA541068 and AA950730. Probable intron in gene represented by EST AA948996.

(Davies, 2001.3.15)

Relationship to Other Genes

Source for database merge of

Source for merge of: CG7814 anon-WO0118547.417

(FlyBase Genome Annotators, 2006, FlyBase, 1996-)

Additional comments

Source for merge of CG7814 anon-WO0118547.417 was sequence comparison ( date:051113 ).

(FlyBase Genome Annotators, 2006, FlyBase, 1996-)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

CG31035

CG34133

(Transgenic RNAi Project members, 2017-, Hosono et al., 2015, Kwon et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Friedman et al., 2011, Keleman et al., 2009.8.5)

CG7814

(Ma et al., 2004, Wayne and McIntyre, 2002, Davies et al., 2001)

anon-WO0118547.417

Name Synonyms

Secondary FlyBase IDs

FBan0007814
FBgn0039715
FBgn0051035
FBgn0062023

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 48 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

318566

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q8IMK1

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q8IMK1

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0083969

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

318566/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0083969

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0083969

FlyMine - An integrated database for Drosophila genomics

FBgn0083969

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG34133

MARRVEL_MODEL - MARRVEL (model organism gene)

318566

Linkouts

BioGRID - A database of protein and genetic interactions.

76884

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0083969

DroID - A comprehensive database of gene and protein interactions.

FBgn0083969

DRSC - Results frm RNAi screens

FBgn0083969

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG34133_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0083969

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0083969

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7814

Flygut - An atlas of the Drosophila adult midgut

FBgn0083969

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC002668

MIST (protein-protein) - An integrated Molecular Interaction Database

318566

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q0KHZ4

References (38)

The following external sites may use different assemblies or annotations than FlyBase.

1.67

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