FlyBase Gene Report: Dmel\can

General Information

Symbol

Dmel\can

Species

D. melanogaster

Name

cannonball

Annotation Symbol

CG6577

Feature Type

protein_coding_gene

FlyBase ID

FBgn0011569

Gene Model Status

Current

Stock Availability

11 publicly available

Gene Summary

cannonball (can) encodes a testis-specific TBP-associated factor essential for spermatogenesis. It is required for the normal level of expression of a large number of spermatogenesis genes and is strongly associated with the nucleolus in primary spermatocytes. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

TAF5L

Key Links

Genomic Location

Cytogenetic map

67D12-67D12

Sequence location

3L:10,263,909..10,267,111 [-]

Recombination map

3-32

RefSeq locus

NT_037436 REGION:10263909..10267111

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (9 terms)

Molecular Function (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (6 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

involved_in male meiosis I

inferred from mutant phenotype

(Lin et al., 1996)

involved_in regulation of gene expression

inferred from mutant phenotype

(White-Cooper et al., 1998)

involved_in spermatid development

inferred from mutant phenotype

(Hiller et al., 2001)

involved_in spermatid differentiation

inferred from mutant phenotype

(White-Cooper et al., 1998)

involved_in spermatogenesis

inferred from mutant phenotype

(Lin et al., 1996)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in transcription initiation at RNA polymerase II promoter

inferred from sequence or structural similarity with SGD:S000000402

(Aoyagi and Wassarman, 2000)

inferred from sequence or structural similarity with UniProtKB:O75529

(Aoyagi and Wassarman, 2000)

inferred from sequence or structural similarity with UniProtKB:Q15542

(Aoyagi and Wassarman, 2000)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in male germ cell nucleus

inferred from direct assay

(Chen et al., 2005)

located_in nucleolus

inferred from direct assay

(Chen et al., 2005)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

part_of transcription factor TFIID complex

inferred from sequence or structural similarity with SGD:S000000402

(Aoyagi and Wassarman, 2000)

inferred from sequence or structural similarity with UniProtKB:O75529

(Aoyagi and Wassarman, 2000)

inferred from sequence or structural similarity with UniProtKB:Q15542

(Aoyagi and Wassarman, 2000)

Gene Group (FlyBase)

TESTIS-SPECIFIC TBP-ASSOCIATED FACTORS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

TESTIS-SPECIFIC TBP-ASSOCIATED FACTORS -: Testis-specific TBP-associated factors (tTAFs) are paralogs of the generally expressed TAF subunits of transcription factor IID (TFIID). They are predominantly localized to spermatocyte nucleoli and regulate the transcription of genes necessary for spermatocyte entry into meiosis. (Adapted from FBrf0200958).

Summary (Interactive Fly)

TAFII80, Trp-Asp repeat (WD-repeat) protein expressed only in spermatocytes, where it is required for transcription of genes required for spermatid differentiation

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\can for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VT91)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.45

Gene model reviewed during 5.56

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

can-RA

FBtr0076299

NM_080088

2953

942

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

can-PA

FBpp0076028

108.5

942

4.64

Q9VT91

NP_524827

AAF50162

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9VT91

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\can using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

can_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

1.40

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

primary spermatocyte

• nucleolus

(Chen et al., 2005)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 5 of adulthood | male -- day 7 of adulthood | male

spermatozoon

(Wasbrough et al., 2010)

Additional Descriptive Data

Turns on specifically in male germ cells soon after initiation of spermatocyte differentiation and persists throughout the remainder of the primary spermatocyte stage. Localizes to the nucleolus.

(Chen et al., 2005)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in male germ cell nucleus

inferred from direct assay

(Chen et al., 2005)

located_in nucleolus

inferred from direct assay

(Chen et al., 2005)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\can in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\can

View exonic expression by tissue for Dmel\can

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

45432/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0011569

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0011569

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0011569

Flygut - An atlas of the Drosophila adult midgut

FBgn0011569

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 17 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 8 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of can

Transgenic constructs containing regulatory region of can

Aberrations (Deficiencies and Duplications) ( 3 )

Inferred from experimentation ( 3 )

Gene not disrupted in

Df(3L)AC1

(Lin et al., 1996)

Df(3L)lxd6

(Lin et al., 1996)

Df(3L)vin2

(Lin et al., 1996)

Inferred from location ( 1 )

Dp(3;2)GV-CH321-14J02

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

can^GD4085

viable, with Scer\GAL4^Mef2.PR

can^GD4085

viable, with Scer\GAL4^pnr-MD237

can^GD4085

Sterility

Allele

male sterile

male sterile | recessive

Other Phenotypes

Allele

abnormal meiotic cell cycle | recessive

Phenotype manifest in

Allele

spermatocyte

testis

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (37)

Hsap\TAF5

7 of 14

Yes

4 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (19)

Rnor\Taf5

7 of 14

Yes

4 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (19)

Mmus\Taf5

7 of 14

Yes

3 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (22)

Xtro\taf5

4 of 13

Yes

3 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (19)

Drer\taf5

7 of 14

Yes

3 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (14)

Cele\taf-5

9 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (15)

Agam\AgaP_AGAP011678

5 of 12

Yes

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (20)

Atha\TAF5

6 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (7)

Scer\TAF5

7 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (13)

Spom\taf5

6 of 12

Yes

Spom\taf73

6 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:can. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (18)

Taf5

9 of 13

wda

4 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

ebi

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

wds

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

can^GD4085

exacerbates Parkinson's disease 8

modeled by Hsap\LRRK2^{G2019S.UAS.Tag:MYC}

(Nucifora et al., 2016)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

TAF5; TATA-box binding protein associated factor 5

7 of 14

601787

TAF5L; TATA-box binding protein associated factor 5 like

4 of 14

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

can - tbrd-1

two hybrid

(Theofel et al., 2014)

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

69670

DroID - A comprehensive database of gene and protein interactions.

FBgn0011569

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Basal transcription factors

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q9VT91

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-32

Cytogenetic map

67D12-67D12

Sequence location

3L:10,263,909..10,267,111 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

67D12-67D12

Limits computationally determined from genome sequence between P{PZ}fry⁰²²⁴⁰ and P{lacW}l(3)L0539^L0539&P{PZ}Dhh1^rL562

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-32

(FlyBase, 2016)

3-24.9

(Comeron et al., 2012)

3-31.0 +/- 1.1

(Lin et al., 1996)

3-31

Left of (cM)

(Lin et al., 1996)

Right of (cM)

(Lin et al., 1996)

Notes

Mapping based on 57 can¹ recombinants between h and rs. can¹ maps proximal to P{lacW}1959.

(Lin et al., 1996)

Stocks and Reagents

Stocks (11)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

74283

w¹¹¹⁸; P{VT028507-p65.AD}attP40

74415

w¹¹¹⁸; P{VT028511-p65.AD}attP40

75541

w¹¹¹⁸; P{VT028507-GAL4.DBD}attP2

75542

w¹¹¹⁸; P{VT028511-GAL4.DBD}attP2

78717

y¹ sc^* v¹ sev²¹; P{TOE.GS02156}attP40

67756

y¹ sc^* v¹ sev²¹; P{TRiP.HMS05778}attP40/CyO

Korea

12673

P{VT028511-GAL4}attP2

NIG-Fly

6577R-3

P{NIG.6577R}3

VDRC

v204314

P{VT028507-GAL4}attP2

v12588

w¹¹¹⁸; P{GD4085}v12588/TM3

v12589

w¹¹¹⁸; P{GD4085}v12589/CyO

Genomic Clones (31)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (1)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

MIP30335

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0011569

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0011569

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

can is required for spermatid differentiation.

(Hiller et al., 2001)

can, mia and sa are required for accumulation of twe^T:Ecol\lacZ transgene in late primary spermatocytes.

(White-Cooper et al., 1998)

Wild type function of aly is required for cell-cycle progression through the G₂/M transition of meiosis I in males and for onset of spermatid differentiation.

(Lin et al., 1996)

can is required for both the progression of the male meiotic cell cycle and the onset of postmeiotic differentiation.

(Lin et al., 1995)

Relationship to Other Genes

Source for database merge of

Source for merge of: can CG6577

(Aoyagi and Wassarman, 2000)

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (14)

Reported As

Symbol Synonym

CG6577

(Fan et al., 2017, Perkins et al., 2009, Aoyagi and Wassarman, 2000)

Can

(Fanara et al., 2023, Laktionov et al., 2018, Theofel et al., 2014, Lu et al., 2013, Lim et al., 2012, Chen et al., 2011)

TAF5

(Cornelio-Parra et al., 2021)

TAF5L

(Helmlinger et al., 2021, Lim et al., 2012, Chen et al., 2011, Tora, 2002, Yu et al., 2002)

Taf5L

(Spedale et al., 2012)

WSB-1

(Nucifora et al., 2016)

WSB1

(Nucifora et al., 2016)

dTAF5L

(Hiller et al., 2004)

dTAF_II80

(Hochheimer and Tjian, 2003)

dmTAF5b

(Hochheimer and Tjian, 2003)

taf5L

(Fan et al., 2017)

Name Synonyms

Cannonball

(Chen et al., 2011, Goodrich and Tjian, 2010, Levine and Tjian, 2003)

cannonball

(Zhan et al., 2013, Lim et al., 2012, Wasbrough et al., 2010, Zhao et al., 2010, Guelman et al., 2006, Hempel et al., 2006)

Secondary FlyBase IDs

FBgn0036079

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 41 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

45432

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

AF346730

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VT91

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q9VT91

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VT91

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

45432/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0011569

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0011569

FlyMine - An integrated database for Drosophila genomics

FBgn0011569

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG6577

MARRVEL_MODEL - MARRVEL (model organism gene)

45432

Linkouts

BioGRID - A database of protein and genetic interactions.

69670

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0011569

DroID - A comprehensive database of gene and protein interactions.

FBgn0011569

DRSC - Results frm RNAi screens

FBgn0011569

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

can_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0011569

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0011569

Flygut - An atlas of the Drosophila adult midgut

FBgn0011569

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0011569

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Basal transcription factors

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q9VT91

References (108)

The following external sites may use different assemblies or annotations than FlyBase.

1.40

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