FlyBase Gene Report: Dmel\Prp19

General Information

Symbol

Dmel\Prp19

Species

D. melanogaster

Name

Pre-RNA processing factor 19

Annotation Symbol

CG5519

Feature Type

protein_coding_gene

FlyBase ID

FBgn0261119

Gene Model Status

Current

Stock Availability

10 publicly available

Gene Summary

Pre-RNA processing factor 19 (Prp19) encodes a protein involved in chromatin organization and alternative mRNA splicing regulation. [Date last reviewed: 2019-07-11] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

l(2)07838, Gbp

Key Links

Genomic Location

Cytogenetic map

55C9-55C9

Sequence location

2R:18,447,361..18,451,041 [-]

Recombination map

2-85

RefSeq locus

NT_033778 REGION:18447361..18451041

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (12 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables ubiquitin protein ligase activity

inferred from electronic annotation with InterPro:IPR038959

(InterPro Project Members, 2004-)

enables ubiquitin-protein transferase activity

inferred from electronic annotation with InterPro:IPR003613

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000356711

(GO Reference Genome Project, 2011-)

inferred from sequence or structural similarity with UniProtKB:Q9UMS4

(Gene Ontology Curators, 2002-)

Biological Process (4 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

involved_in chromatin organization

inferred from mutant phenotype

(Klusza et al., 2013)

involved_in regulation of alternative mRNA splicing, via spliceosome

inferred from mutant phenotype

(Ashton-Beaucage et al., 2014)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in mRNA splicing, via spliceosome

inferred from electronic annotation with InterPro:IPR038959

(InterPro Project Members, 2004-)

inferred by curator from GO:0071011,GO:0071013

(Herold et al., 2009)

traceable author statement

(Milchanowski et al., 2004)

inferred from biological aspect of ancestor with PANTHER:PTN000356711

(GO Reference Genome Project, 2011-)

involved_in protein ubiquitination

inferred from sequence or structural similarity with UniProtKB:Q9UMS4

(Gene Ontology Curators, 2002-)

inferred from electronic annotation with InterPro:IPR003613

(InterPro Project Members, 2004-)

Cellular Component (6 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

part_of catalytic step 2 spliceosome

inferred from high throughput direct assay

(Herold et al., 2009)

part_of precatalytic spliceosome

inferred from high throughput direct assay

(Herold et al., 2009)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000356711

(GO Reference Genome Project, 2011-)

part_of Prp19 complex

inferred from biological aspect of ancestor with PANTHER:PTN000356711

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR038959

(InterPro Project Members, 2004-)

inferred from sequence or structural similarity with UniProtKB:Q9UMS4

(Gene Ontology Curators, 2002-)

part_of spliceosomal complex

traceable author statement

(Milchanowski et al., 2004)

part_of U2-type catalytic step 1 spliceosome

inferred from biological aspect of ancestor with PANTHER:PTN000356711

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

U-BOX UBIQUITIN LIGASES

SPLICEOSOME COMPLEX B

SPLICEOSOME COMPLEX C

SPLICEOSOME COMPLEX P

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Pre-RNA processing factor 19 (Prp19) encodes a protein involved in chromatin organization and alternative mRNA splicing regulation. [Date last reviewed: 2019-07-11]

Gene Group (FlyBase)

U-BOX UBIQUITIN LIGASES -: E3 (ubiquitin) ligases interact with both ubiquitin-E2 (ubiquitin-conjugating) enzymes and the target protein, transferring the ubiquitin from the E2 to the substrate. U-box ubiquitin ligases (UULs) possess a domain structurally related the RING finger domain found in many E3s, but lacks Zn²⁺-co-ordinating residues. Some UULs have been found to possess E4 activity, co-ordinating with E1, E2 and E3 enzymes to transfer polyubiquitin to target proteins. (Adapted from PMID:20979629 and PMID:16250894).
SPLICEOSOME COMPLEX B -: Nuclear pre-mRNA splicing is catalyzed by the spliceosome which assembles in a step-wise manner. After the assembly of complex A on pre-mRNA, the U4-U6 and U5 snRNPs are recruited as a preassembled tri-snRNP to form complex B. U5 snRNP binds exons at the 5' site. (Adapted from PMID:24452469 and PMID:23118483).
SPLICEOSOME COMPLEX C -: Nuclear pre-mRNA splicing is catalyzed by the spliceosome which assembles in a step-wise manner. Complex B undergoes a number of structural rearrangements and U4 and U1 snRNPs dissociate to generate complex C, the catalytic spliceosome. (Adapted from PMID:24452469 and PMID:23118483).
SPLICEOSOME COMPLEX P -: Nuclear pre-mRNA splicing is catalyzed by the spliceosome which assembles in a step-wise manner. Complex P, the post-spliceosomal complex, is formed after the catalytic removal of the intron by complex C. Complex P contains the lariat intron and spliced exons. (Adapted from PMID:24452469 and PMID:23118483).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Prp19 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q7KLW9)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.50

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Prp19-RA

FBtr0086723

NM_079059

1757

505

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Prp19-PA

FBpp0085902

55.2

505

6.90

Q7KLW9

NP_523783

AAF57684

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Prp19 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Prp19_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.76

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of catalytic step 2 spliceosome

inferred from high throughput direct assay

(Herold et al., 2009)

part_of precatalytic spliceosome

inferred from high throughput direct assay

(Herold et al., 2009)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Prp19 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Prp19

View exonic expression by tissue for Dmel\Prp19

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37123/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0261119

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0013969

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0261119

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5519

Flygut - An atlas of the Drosophila adult midgut

FBgn0261119

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 8 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 9 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Prp19

Transgenic constructs containing regulatory region of Prp19

Aberrations (Deficiencies and Duplications) ( 4 )

Inferred from experimentation ( 4 )

Gene disrupted in

Df(2R)PC4

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Df(2R)PC29

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Df(2R)RM2-1

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Gene not disrupted in

Df(2R)P34

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Inferred from location ( 4 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^elav.PU

lethal, with Scer\GAL4^nub-AC-62

Prp19^HMS00652

lethal, with Scer\GAL4^pnr-MD237

Prp19^GD11681

lethal | female limited, with Scer\GAL4^dome-PG14

Prp19^HMS00652

lethal | pupal stage, with Scer\GAL4^pnr-MD237

Prp19^GD11681

viable, with Scer\GAL4^tin.CΔ4

Prp19^GD11681

Sterility

Allele

female sterile, with Scer\GAL4^VP16.otu

Prp19^HMS00652

Other Phenotypes

Allele

abnormal behavior | adult stage, with Scer\GAL4^elav.PU

Prp19^HMS00652

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Prp19^GD11681

abnormal size | third instar larval stage, with Scer\GAL4^Cg.PA

Prp19^HMS00652

decreased occurrence of cell division | larval stage, with Scer\GAL4^elav.PU

Prp19^HMS00652

decreased rate of larval locomotory behavior, with Scer\GAL4^elav.PU

Prp19^HMS00652

decreased size | adult stage, with Scer\GAL4^GMR.PF

Prp19^RNAi.UAS.cUa

decreased size | larval stage, with Scer\GAL4^elav.PU

Prp19^HMS00652

long lived | female | RU486 conditional, with Scer\GAL4^da.Switch.PT

Prp19^UAS.cGa

long lived | female | RU486 conditional, with Scer\GAL4^{αTub84B.Switch.PK}

Prp19^UAS.cGa

visible | adult stage, with Scer\GAL4^GMR.PF

Prp19^RNAi.UAS.cUa

Phenotype manifest in

Allele

chromatin, with Scer\GAL4^{VP16.mat.αTub67C}

Prp19^HMS00652

embryonic/larval fat body | third instar larval stage, with Scer\GAL4^Cg.PA

Prp19^HMS00652

eye, with Scer\GAL4^GMR.PF

Prp19^RNAi.UAS.cUa

germarium, with Scer\GAL4^nanos.PU

Prp19^HMS00652

larval brain | larval stage, with Scer\GAL4^elav.PU

Prp19^HMS00652

lobe system of larval mushroom body | larval stage, with Scer\GAL4^elav.PU

Prp19^HMS00652

mitochondrion | third instar larval stage, with Scer\GAL4^Cg.PA

Prp19^HMS00652

nucleolus, with Scer\GAL4^{VP16.mat.αTub67C}

Prp19^HMS00652

nurse cell, with Scer\GAL4^{VP16.mat.αTub67C}

Prp19^HMS00652

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (24)

Hsap\PRPF19

14 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (11)

Rnor\Prpf19

14 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (9)

Mmus\Prpf19

13 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (22)

Xtro\prpf19

7 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\LOC100490380

1 of 13

Xtro\LOC100491248

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\wdr5

1 of 13

Danio rerio (Zebrafish) (10)

Drer\prpf19

14 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (16)

Cele\prp-19

13 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Cele\Y44F5A.1

1 of 14

Cele\Y45F10D.7

1 of 14

Cele\Y111B2A.12

1 of 14

Anopheles gambiae (African malaria mosquito) (22)

Agam\AgaP_AGAP007217

11 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (17)

Atha\MAC3A

13 of 13

Yes

Atha\MAC3B

13 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (8)

Scer\PRP19

9 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (7)

Spom\prp19

11 of 12

Yes

Spom\caf4

1 of 12

Yes

Spom\mip1

1 of 12

Spom\pfs2

1 of 12

Spom\raf1

1 of 12

Yes

Spom\SPAC1A6.02

1 of 12

Spom\SPCC18.15

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Prp19. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (18)

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

gro

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

wcd

1 of 13

Wdr20

1 of 13

wds

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

neurodevelopmental disorder, PRPF19-related

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Allele

Disease

Evidence

References

Prp19^HMS00652

model of syndromic intellectual disability

CEC

(Li et al., 2024)

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 2 )

Allele

Disease

Interaction

References

Prp19^GD11681

exacerbates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Chen et al., 2021)

Prp19^G3080

ameliorates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Chen et al., 2021)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

PRPF19; pre-mRNA processing factor 19

14 of 14

PRE-mRNA-PROCESSING FACTOR 19; PRPF19

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Dmel gene

Ortholog showing functional complementation

Supporting References

Prp19

Hsap\PRPF19

(Li et al., 2024)

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Prp19 - BCAS2

anti tag coimmunoprecipitation, peptide massfingerprinting

(Guilgur et al., 2014)

Prp19 - Bx42

anti tag coimmunoprecipitation, peptide massfingerprinting

(Guilgur et al., 2014)

Prp19 - CG9667

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, peptide massfingerprinting

(Guilgur et al., 2014)

Prp19 - Cdc5

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, peptide massfingerprinting

(Guilgur et al., 2014)

Prp19 - Myc

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Prp19 - Pi3K21B

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Prp19 - RasGAP1

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Prp19 - S6kII

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Prp19 - Tango4

anti tag coimmunoprecipitation, peptide massfingerprinting

(Guilgur et al., 2014)

Prp19 - Tsc1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Prp19 - U2af50

anti bait coimmunoprecipitation, western blot

(Minocha et al., 2018)

Prp19 - cyp33

anti tag coimmunoprecipitation, peptide massfingerprinting

(Guilgur et al., 2014)

Prp19 - fand

anti tag coimmunoprecipitation, peptide massfingerprinting, anti bait coimmunoprecipitation, western blot

(Minocha et al., 2018, Guilgur et al., 2014)

Prp19 - foxo

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Prp19 - ft

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kwon et al., 2013)

Prp19 - gig

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Prp19 - rictor

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Prp19 - salsa

anti tag coimmunoprecipitation, peptide massfingerprinting, western blot

(Rathore et al., 2020, Guilgur et al., 2014)

Prp19 - sima

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Prp19

enhanceable

Atg1

(Umargamwala et al., 2025)

Prp19

suppressible

Rbfox1

(Li et al., 2024)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Atg1

enhanceable

Prp19

(Umargamwala et al., 2025)

cindr

suppressible

Prp19

(Ketosugbo et al., 2017)

cnk

enhanceable

Prp19

(Baril et al., 2014)

Ras85D

suppressible

Prp19

(Ashton-Beaucage et al., 2014)

enhanceable

Prp19

(Ashton-Beaucage et al., 2014)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

62799

DroID - A comprehensive database of gene and protein interactions.

FBgn0261119

MIST (protein-protein) - An integrated Molecular Interaction Database

37123

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q24055

Class of Gene

gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-85

Cytogenetic map

55C9-55C9

Sequence location

2R:18,447,361..18,451,041 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

55C9-55C9

Limits computationally determined from genome sequence between P{lacW}l(2)08770^k04808 and P{EP}EP437

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

55D1-55D2

(determined by in situ hybridisation)

(Spradling et al., 1999)

55D1-55D2

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

2-85

(FlyBase, 2016)

2-89.9

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (10)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

28455

y¹ w^*; P{EP}Prp19^G3080

32865

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00652}attP2

79322

y¹ w^*; TI{CRIMIC.TG4.2}Prp19^{CR00856-TG4.2} CG30120^{CR00856-TG4.2-X} Lamtor2^{CR00856-TG4.2-X}/SM6a

NIG-Fly

5519R-1

P{NIG.5519R}1

5519R-4

P{NIG.5519R}4

HMS00652

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00652}attP2

2RG-0945

y² VhaAC39-1^cho-1 v¹; P{U6-Prp19.NIG.sgRNA}attP40/CyO

VDRC

v108575

P{KK101335}VIE-260B

v41438

w¹¹¹⁸; P{GD11681}v41438

v318845

PBac{fTRG01327.sfGFP-TVPTBF}VK00033

Genomic Clones (14)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (51)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD02793

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0261119

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0261119

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Minocha et al., 2018, Guilgur et al., 2014)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Relationship to Other Genes

Source for database merge of

Source for merge of: Prp19 CG5519

(Spradling et al., 1999)

Source for merge of: Gbp BcDNA:LD02793

(FlyBase, 1996-)

Additional comments

Source for merge of Gbp BcDNA:LD02793 was sequence comparison ( date:000202 ).

(FlyBase, 1996-)

Identified only as a PCR sequence; identity to other GTP binding protein encoding genes unknown.

(Winge, 1995.3.30)

Nomenclature History

Source for database identify of

Nomenclature comments

There is no evidence that CG5519 encodes a GTP binding protein. FBrf0122136 seems to have mis-identified it as a G-protein, based only on the fact that it has WD40 repeats. For these reasons, the FlyBase symbol/name for this gene has reverted from 'Gbp/GTP-binding-protein' to CG5519.

(Peifer, 2009.3.17)

Etymology

Synonyms and Secondary IDs (15)

Reported As

Symbol Synonym

BcDNA:LD02793

CG5519

(Sun et al., 2023, National Institute of Genetics Fly Stocks, 2022-, Kolkhof et al., 2017, Hosono et al., 2015, Rosa-Ferreira et al., 2015, Guilgur et al., 2014, O'Keefe et al., 2011, Ying et al., 2011, Ni et al., 2010.12.1, Herold et al., 2009, Keleman et al., 2009.8.5, Andersen and Tapon, 2008, Haussmann et al., 2008, Hughes et al., 2008)

EC2-11

(Baril et al., 2014)

GPB

(Winge, 1995.3.30)

Gbp

(Herold et al., 2009, Fisher et al., 2008)

PRP19

(Marin, 2010)

Prp191

(Lin et al., 2024)

dPRP19

(Promislow et al., 2022)

dPrp19

(Garschall et al., 2017)

l(2)07838

(Beaton, 2000.1.31, Meister and Braun, 1995.10, BDGP Project Members, 1994-1999)

prp19

(Andersen and Tapon, 2008)

Name Synonyms

GTP-binding regulatory protein beta chain

(Winge, 1995.3.30)

NM 079059.2

(Marin, 2010)

Pre-RNA processing factor 19

Secondary FlyBase IDs

FBgn0010644
FBgn0013969
FBgn0027900

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 50 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

37123

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q7KLW9

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q7KLW9

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37123/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0261119

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0261119

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG5519

MARRVEL_MODEL - MARRVEL (model organism gene)

37123

Linkouts

BioGRID - A database of protein and genetic interactions.

62799

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0261119

DroID - A comprehensive database of gene and protein interactions.

FBgn0261119

DRSC - Results frm RNAi screens

FBgn0261119

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Prp19_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0013969

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0261119

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5519

Flygut - An atlas of the Drosophila adult midgut

FBgn0261119

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0261119

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC034425

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

MIST (protein-protein) - An integrated Molecular Interaction Database

37123

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q24055

References (94)

The following external sites may use different assemblies or annotations than FlyBase.

-0.76

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