FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000099417
Interaction Network
Interactions Browser links
Taf5 network
Taf4 network
Reported Interactions
FBrf0137329-25.coIP.WB
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Taf4 

dTAFII110

bait
Taf5 

dTAFII85

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Partially purified Bio-Rex70 fractions were used for this immunoprecipitation study.

FBrf0137329-31.coIP.WB
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Taf5 

dTAFII85

bait
Taf4 

dTAFII110

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

The interaction of Taf5 with Taf4 is not dependent on DNA, as ethidium bromide does not disrupt their association.

Partially purified Bio-Rex70 fractions were used for this immunoprecipitation study.

FBrf0192616-2.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Taf4 

TAF4

bait
Taf5 

TAF5

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0192616-7.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Taf4 

TAF4

bait

FLAG tag

Taf4 
Taf5 

TAF5

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Taf4 
CTR domain
sufficient binding region
aa 623-921

coordinates relative to Taf4-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from tagged transfected construct; prey produced from endogenous gene.

FBrf0230158-1.coIP.WB
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Taf5 

Taf5

bait
Taf4 

Taf4

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larvae of wild-type or Taf10 mutant fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Interaction occurs in both wild-type and Taf10, Taf10b double mutant.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)