FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
hpo, Cka
FlyBase ID
FBig0000086124
Interaction Network
Interactions Browser links
hpo network
Cka network
Reported Interactions
FBrf0211636-26.coIP.MS
Reference
Ribeiro et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc167
Participants
Corresponds to
Reported as
Role
Note
Cka 

Cka

bait

FLAG tag

hpo 

Hpo

prey

20 unique peptides identified

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0211636-39.coIP.WB
Reference
Ribeiro et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
hpo 

Hbo

bait

FLAG tag

Cka 

Cka

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0211636-44.coIP.WB
Reference
Ribeiro et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Cka 

Cka

bait

FLAG tag

hpo 

Hpo

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Serial coIP experiments indicate that Cka, hpo, and CG10158 proteins associate as a trimeric complex.

(Ribeiro et al., 2010)
FBrf0211636-5.coIP.MS
Reference
Ribeiro et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc167
Participants
Corresponds to
Reported as
Role
Note
hpo 

Hbo

bait

FLAG tag

Cka 

Cka

prey

10 unique peptides identified

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0237510-5.coIP.WB
Reference
Zheng et al., 2017
Description
physical association
Assay
Collection
Source/Stage
organism / larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cka 

cka

bait

FLAG tag

hpo 

Hpo

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larvae of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Zheng et al., 2017)
FBrf0237510-8.coIP.WB
Reference
Zheng et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Cka 

Cka

bait

FLAG tag

hpo 

Hpo

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zheng et al., 2017)

The Cka-hpo interaction is weak.

(Zheng et al., 2017)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)