FlyBase Allele Report: Dmel\Cka[GD12225]

General Information

Symbol

Dmel\Cka^GD12225

Species

D. melanogaster

Name

FlyBase ID

FBal0206587

Feature type

allele

Associated gene

Dmel\Cka

Associated Insertion(s)

Carried in Construct

P{GD12225}

Also Known As

Cka RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD12225}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD12225

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

cyst cell of testis | third instar larval stage, with Scer\GAL4^C587

(La Marca et al., 2019)

dendrite, with Scer\GAL4⁴⁷⁷, Scer\GAL4^ppk.PU

(Bhattacharjee et al., 2022)

dendritic branch | decreased number, with Scer\GAL4⁴⁷⁷, Scer\GAL4^ppk.PU

(Bhattacharjee et al., 2022)

gonad | male | third instar larval stage, with Scer\GAL4^C587

(La Marca et al., 2019)

indirect flight muscle cell | pupal stage, with Scer\GAL4^Mef2.PR

(Kaya-Çopur et al., 2021)

male germline cyst | third instar larval stage, with Scer\GAL4^C587

(La Marca et al., 2019)

male germline stem cell | third instar larval stage, with Scer\GAL4^C587

(La Marca et al., 2019)

multidendritic neuron, with Scer\GAL4⁴⁷⁷, Scer\GAL4^ppk.PU

(Bhattacharjee et al., 2022)

myotube | pupal stage, with Scer\GAL4^Mef2.PR

(Kaya-Çopur et al., 2021)

spermatogonium | third instar larval stage, with Scer\GAL4^C587

(La Marca et al., 2019)

Detailed Description

Statement

Reference

The expression of Cka^GD12225 under the control of Scer\GAL4^Mef2.PR leads to indirect flight muscle atrophy during pupal development (after 32h APF); fibers are longer than in controls and fail to compact at 32h APF.

(Kaya-Çopur et al., 2021)

Expressing Cka^GD12225 under the control of Scer\GAL4^C587 (together with Dicer-2, for a more efficient RNAi) leads to L3 male gonad defects: small gonads with supernumerary strongly DAPI-positive cells throughout, whereas control gonads exhibit brighter DAPI-stained DNA in the apical region of the tissue, where the GSCs, gonialblasts, TA spermatogonia, CySCs and CyCs reside. Further analysis uncovers additional defects in germline differentiation and somatic cell enclosure of cyst.

(La Marca et al., 2019)

Adults expressing Cka^GD12225 under the control of the cardioblast-specific Scer\GAL4^tin.CΔ4 driver show significantly reduced survival on day 6 after a shift to 29[o]C compared to control flies.

(Neely et al., 2010)

Adults expressing Cka^GD12225 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression of Cka^GD12225 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) results in viable flies or partial lethality, depending on the particular P{GD12225} insertion line used.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in early pupal lethality or late pupal lethality.

(Schnorrer et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development