FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
SAK, Ubi-p5E
FlyBase ID
FBig0000104351
Interaction Network
Interactions Browser links
SAK network
Ubi-p5E network
Reported Interactions
FBrf0227526-5.coIP.WB
Reference
Klebba et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
SAK 

Pkl4

bait

EGFP tag

Ubi-p5E 

Ubi

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The addition of asl stabilizes the interaction between SAK and Ubi-p5E but does not prevent SAK from being ubiquinated.

(Klebba et al., 2015)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Klebba et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)