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Citation
McLamarrah, T.A., Speed, S.K., Ryniawec, J.M., Buster, D.W., Fagerstrom, C.J., Galletta, B.J., Rusan, N.M., Rogers, G.C. (2020). A molecular mechanism for the procentriole recruitment of Ana2.  J. Cell Biol. 219(2): e201905172.
FlyBase ID
FBrf0246771
Publication Type
Research paper
Abstract

During centriole duplication, a preprocentriole forms at a single site on the mother centriole through a process that includes the hierarchical recruitment of a conserved set of proteins, including the Polo-like kinase 4 (Plk4), Ana2/STIL, and the cartwheel protein Sas6. Ana2/STIL is critical for procentriole assembly, and its recruitment is controlled by the kinase activity of Plk4, but how this works remains poorly understood. A structural motif called the G-box in the centriole outer wall protein Sas4 interacts with a short region in the N terminus of Ana2/STIL. Here, we show that binding of Ana2 to the Sas4 G-box enables hyperphosphorylation of the Ana2 N terminus by Plk4. Hyperphosphorylation increases the affinity of the Ana2-G-box interaction, and, consequently, promotes the accumulation of Ana2 at the procentriole to induce daughter centriole formation.

PubMed ID
PubMed Central ID
PMC7041687 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    J. Cell Biol.
    Title
    Journal of Cell Biology
    Publication Year
    1966-
    ISBN/ISSN
    0021-9525
    Data From Reference
    Genes (6)
    Physical Interactions (17)
    Cell Lines (1)