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General Information
Symbol
S2-DRSC
Species
D. melanogaster
Feature type
FlyBase ID
FBtc0000181
Source
Tissue Source
Lab of Origin

Schneider

Characterization
Sex
male (based on expression levels of roX genes, msl-2 and traF; splicing patterns of tra and Sxl)
Karyotype
Integrated Constructs (0)
    Comments

    Stable cell line generated: S2 cells expressing Tag:FLAG,Tag:HA-tagged not under the control of the MT promoter.

    Stable cell line generated: A stable S2 cell line was created containing a FBto0000031:GFP reporter under the control of the Mmp1 promoter.

    Stable cell line generated: A stable S2 cell line co-expressing l(2)gl-RFP(Unk) and l(2)gl-GFP was created. A stable S2 cell line expressing l(2)gl-Dendra2 was created. A stable S2 cell line expressing l(2)gl-GFP and mCherry-tubulin (FBrf0227229) was used.

    Stable cell line generated: An S2 cell line stably co-expressing l(2)gl.3A-RFP and l(2)gl-GFP and an S2 cell line stably expressing l(2)gl-Dendra2 were created.

    Stock #181 from DGRC

    Expression profiling by genome tiling array for this cell line (response to 5 hr ecdysone treatment) may be found at GEO: GSE16269 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE11280).

    Expression of cell-type specific markers correlates with that of plasmocytes and crystal cells; likely that this lines contains a mixture of cell types.

    Expression profiling by genome tiling array for this cell line may be found at GEO: GSE16286 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE16286).

    RNA-Seq transcription profile for this cell line may be found at GEO: GSE15596 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE15596). See also the FlyBase collection report modENCODE_mRNA-Seq_cell.A (FBlc0000116) and FlyBase RNA-Seq GBrowse view.

    S2 cells stably expressing an inducible construct with the signal sequence of Drosophila Bip appended to Arus\HRP were used to monitor secretion.

    S2-DRSC is one of the four lines chosen for extensive analysis by the modENCODE project.

    This S2 isolate is the one routinely used for RNAi screens at the DRSC; it is referred to at the DRSC as "S2". Its relationship to the S2-DGRC line is not known.

    Stable cell line generated: S2 cells stably expressing the signal sequence of Drosophila Bip appended to Arus\HRP and a Tag:V5 tag under the influence of an inducible MtnA promoter were generated as a way to monitor secretion.

    Parental Lines (1)
    Isolate of:
    Descendant Lines (0)
     
    External Stocks and Resources ( 1 )
    Crossreferences
    DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
    Synonyms and Secondary IDs (10)
    Reported As
    Symbol Synonym
    CVCL_Z992
    RRID:CVCL_Z992
    Schneider's 2
    Schneider's line 2
    Name Synonyms
    Secondary FlyBase IDs
      References (141)