FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Gene: Dmel\mir-8
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General Information
Symbol
Dmel\mir-8
Species
D. melanogaster
Name
mir-8 stem loop
Annotation Symbol
CR42988
Feature Type
miRNA_gene
FlyBase ID
FBgn0262432
Gene Model Status
Current
Stock Availability
5 publicly available
Gene Summary
acts through U-shaped to activate PI3K, thereby promoting fat cell growth cell-autonomously and enhancing growth non-cell-autonomously - antagonizes Wg signaling in part by directly targeting wntless, a gene required for Wg secretion (Interactive Fly, mir-8 )
Contribute a Gene Snapshot for this gene.
All Summaries
Also Known As

dme-miR-8, mir-8S, miR8

Key Links
Genomic Location
Cytogenetic map
53D11-53D11
Sequence location
Recombination map
2-80
RefSeq locus
NT_033778 REGION:16831432..16831518
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (28 terms)
Molecular Function (2 terms)
Biological Process (25 terms)
Terms Based on Experimental Evidence (25 terms)
CV Term
Evidence
References
involved_in eclosion
inferred from mutant phenotype
(Karres et al., 2007)
involved_in glial cell growth
inferred from mutant phenotype
(Morante et al., 2013)
involved_in host-mediated suppression of viral genome replication
inferred from mutant phenotype
(Monsanto-Hearne et al., 2017)
involved_in mesenchymal to epithelial transition
inferred from mutant phenotype
(Antonello et al., 2015, Antonello et al., 2015)
involved_in miRNA-mediated gene silencing by inhibition of translation
inferred from direct assay
(Vallejo et al., 2011, Kennell et al., 2008)
involved_in miRNA-mediated gene silencing by mRNA destabilization
inferred from direct assay
(Monsanto-Hearne et al., 2017, Antonello et al., 2015, Lee and Hyun, 2014, Loya et al., 2014, Hyun et al., 2009, Karres et al., 2007)
involved_in miRNA-mediated post-transcriptional gene silencing
inferred from direct assay
(Guo et al., 2020, Lim et al., 2020, Sander et al., 2018, Eichenlaub et al., 2016, Morante et al., 2013, Nesler et al., 2013, Kennell et al., 2008)
inferred from mutant phenotype
(Loya et al., 2009, Karres et al., 2007)
involved_in negative regulation of canonical Wnt signaling pathway
inferred from mutant phenotype
(Kennell et al., 2008)
involved_in negative regulation of epidermal growth factor receptor signaling pathway
inferred from genetic interaction with FLYBASE:spi; FB:FBgn0005672
(Morante et al., 2013)
involved_in negative regulation of gene expression
inferred from mutant phenotype
(Lee et al., 2015)
involved_in negative regulation of growth
inferred from genetic interaction with FLYBASE:yki; FB:FBgn0034970
(Sander et al., 2018)
involved_in negative regulation of mitotic cytokinesis
inferred from mutant phenotype
(Eichenlaub et al., 2016)
involved_in negative regulation of neurogenesis
inferred from mutant phenotype
(Morante et al., 2013)
involved_in negative regulation of Notch signaling pathway
inferred from mutant phenotype
(Vallejo et al., 2011)
involved_in negative regulation of Toll signaling pathway
inferred from mutant phenotype
(Lee and Hyun, 2014)
involved_in negative regulation of Wnt protein secretion
inferred from mutant phenotype
(Kennell et al., 2008)
involved_in positive regulation of eclosion
inferred from mutant phenotype
(Kennell et al., 2012)
involved_in positive regulation of female pigmentation
inferred from mutant phenotype
(Kennell et al., 2012)
involved_in positive regulation of hippo signaling
inferred from mutant phenotype
(Guo et al., 2020, Sander et al., 2018)
involved_in positive regulation of insulin receptor signaling pathway
inferred from mutant phenotype
(Hyun et al., 2009)
involved_in positive regulation of multicellular organism growth
inferred from mutant phenotype
(Lee et al., 2015, Hyun et al., 2009)
involved_in positive regulation of synaptic assembly at neuromuscular junction
inferred from mutant phenotype
(Loya et al., 2014, Loya et al., 2009)
involved_in regulation of imaginal disc-derived leg joint morphogenesis
inferred from mutant phenotype
(Karres et al., 2007)
involved_in regulation of synaptic assembly at neuromuscular junction
inferred from mutant phenotype
(Nesler et al., 2013)
involved_in subsynaptic reticulum organization
inferred from mutant phenotype
(Loya et al., 2014)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in miRNA-mediated post-transcriptional gene silencing
inferred from electronic annotation with Rfam:RF00241
(RNAcentral, 2018-)
Cellular Component (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
part_of RISC complex
inferred from electronic annotation with Rfam:RF00241
(RNAcentral, 2018-)
Gene Group (FlyBase)
Pathway (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
    -
    Summaries
    Pathway (FlyBase)
    NEGATIVE REGULATORS OF WNT-TCF SIGNALING PATHWAY -
    Negative regulators of Wnt-TCF (canonical Wnt) signaling down-regulate the pathway, resulting in the attenuation of transcriptional regulation mediated by β-catenin (arm).
    POSITIVE REGULATORS OF INSULIN-LIKE RECEPTOR SIGNALING PATHWAY -
    Positive regulators of the Insulin-like Receptor signaling pathway enhance Insulin-like receptor (InR) activation or the activity of intracellular effectors. (Adapted from FBrf0232297, FBrf0230017 and FBrf0229989).
    POSITIVE REGULATORS OF HIPPO SIGNALING PATHWAY -
    The Hippo signaling pathway is an intracellular kinase cascade in which hpo kinase in complex with sav, phosphorylates wts kinase which, in turn, phosphorylates yki transcriptional co-activator leading to its cytosolic retention. Positive regulators of the pathway, enhance the cytosolic retention of yki, negatively regulating tissue growth (Adapted from FBrf0224870).
    NEGATIVE REGULATORS OF EGFR SIGNALING PATHWAY -
    Negative regulators of Epidermal Growth Factor Receptor signaling down-regulate the pathway, suppressing the activation of ERK kinase (rl) or acting on downstream effectors.
    NEGATIVE REGULATORS OF TOLL-NF-kappaB SIGNALING PATHWAY -
    In Drosophila, the canonical Toll signaling pathway is initiated by the binding of a spatzle ligand to Toll (Tl) or a Toll-like receptor leading to the nuclear localization of the NF-κB (dl or Dif) transcription factor. Negative regulators of Toll-NF-κB Signaling lead to the increased cytosolic retention of the NF-κB proteins dl or Dif and/or repression of target gene transcription. (Adapted from FBrf0091014 and FBrf0223077).
    NEGATIVE REGULATORS OF NOTCH SIGNALING PATHWAY -
    The Notch receptor signaling pathway is activated by the binding of the transmembrane receptor Notch (N) to transmembrane ligands, Dl or Ser, presented on adjacent cells. This results in the proteolytic cleavage of N, releasing the intracellular domain (NICD). NICD translocates into the nucleus, interacting with Su(H) and mam to form a transcription complex, which up-regulates transcription of Notch-responsive genes. Negative regulators of the pathway down-regulate the signal from the sending cell or the response in the receiving cell. (Adapted from FBrf0225731 and FBrf0192604).
    Summary (Interactive Fly)

    acts through U-shaped to activate PI3K, thereby promoting fat cell growth cell-autonomously and enhancing growth non-cell-autonomously - antagonizes Wg signaling in part by directly targeting wntless, a gene required for Wg secretion

    Gene Model and Products
    Number of Transcripts
    3
    Number of Unique Polypeptides
    0

    Please see the JBrowse view of Dmel\mir-8 for information on other features

    To submit a correction to a gene model please use the Contact FlyBase form

    Protein Domains (via Pfam)
    Isoform displayed:
    Pfam protein domains
    InterPro name
    classification
    start
    end
    Protein Domains (via SMART)
    Isoform displayed:
    SMART protein domains
    InterPro name
    classification
    start
    end
    Structure
    Experimentally Determined Structures
    Crossreferences
    Comments on Gene Model

    Locations and structures of miRNA models as mapped by miRBase (FBrf0220601).

    Conserved: found throughout the Drosophila genus (FBrf0230987).

    Transcript Data
    Annotated Transcripts
    Name
    FlyBase ID
    RefSeq ID
    Length (nt)
    mir-8-RA
    FBtr0304405
    NR_048129
    23
    mir-8-RB
    FBtr0472709
    22
    mir-8-RM
    FBtr0304404
    NR_048128
    87
    Additional Transcript Data and Comments
    Reported size (kB)
    Comments
    External Data
    Crossreferences
    miRBase - A searchable database of published miRNA sequences and annotation.
    Rfam - A collection of RNA sequence families of structural RNAs including non-coding RNA genes as well as cis-regulatory elements
    Sequences Consistent with the Gene Model
    Nucleotide / Polypeptide Records
    Mapped Features

    Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\mir-8 using the Feature Mapper tool.

    External Data
    Crossreferences
    Linkouts
    Expression Data
    Testis-specificity index

    The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

    NA

    Transcript Expression
    in situ
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    embryonic stage 10
    yolk nucleus
    (Aboobaker et al., 2005)
    embryonic stage 11
    embryonic primordium of adult salivary gland
    (Aboobaker et al., 2005)
    hindgut anlage | proximal to
    (Aboobaker et al., 2005)
    embryonic stage 11 -- 12
    trunk mesoderm | subset
    (Aboobaker et al., 2005)
    embryonic stage 12
    embryonic/larval salivary gland
    (Aboobaker et al., 2005)
    embryonic stage 12 -- 13
    ectoderm | segmentally repeated
    (Aboobaker et al., 2005)
    embryonic stage 13
    embryonic/larval posterior midgut
    (Aboobaker et al., 2005)
    third instar larval stage
    larval brain | restricted
    (Morante et al., 2013)
    northern blot
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    embryonic stage -- adult stage
    (Aravin et al., 2003, Sempere et al., 2003)
    third instar larval stage
    organism

    Comment: high

    (Hyun et al., 2009)
    fat body

    Comment: highest

    (Hyun et al., 2009)
    gut

    Comment: lowest

    (Hyun et al., 2009)
    brain

    Comment: low

    (Hyun et al., 2009)
    cuticle

    Comment: high

    (Hyun et al., 2009)
    Additional Descriptive Data

    mir-8-mRNA is detected in a subset of cells throughout the larval nervous system.

    (Morante et al., 2013)

    Expression is strong from 12 hour embryos though adults, with a peak in 12-24 hour embryos through first instar larvae. Expression is weak in 0-4 hour embryos.

    (Aravin et al., 2003)

    Northern analysis of 24 microRNAs showed that a number of them were expressed constituitively throughout development.

    (Sempere et al., 2003)
    Marker for
     
    Subcellular Localization
    CV Term
    Polypeptide Expression
    Additional Descriptive Data
    Marker for
     
    Subcellular Localization
    CV Term
    Evidence
    References
    Expression Deduced from Reporters
    High-Throughput Expression Data
    Associated Tools

    JBrowse - Visual display of RNA-Seq signals

    View Dmel\mir-8 in JBrowse
    RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
    Bulk Downloads
    RNA-Seq RPKM values for all genes
    Reference
    See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
    Developmental Proteome: Life Cycle
    Developmental Proteome: Embryogenesis
    External Data and Images
    Linkouts
    DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
    EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
    FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
    Images
    FlyBase Wiki
    Image Based Resources
    Alleles, Insertions, Transgenic Constructs, and Aberrations
    Classical and Insertion Alleles ( 11 )
    For All Classical and Insertion Alleles Show
     
    Other relevant insertions
    Transgenic Constructs ( 14 )
    For All Alleles Carried on Transgenic Constructs Show
    Transgenic constructs containing/affecting coding region of mir-8
    Transgenic constructs containing regulatory region of mir-8
    Aberrations (Deficiencies and Duplications) ( 2 )
    Inferred from experimentation ( 2 )
    Gene disrupted in
    Df(2R)mir-8Δ3
    (Karres et al., 2007)
    Gene not disrupted in
    Df(2R)CG6301-Δ
    (Lu et al., 2014)
    Inferred from location ( 6 )
    Variants
    Variant Molecular Consequences
    Alleles Representing Disease-Implicated Variants
    Phenotypes
    For more details about a specific phenotype click on the relevant allele symbol.
    Lethality
    Allele
    lethal
    lethal, with Scer\GAL41151
    lethal, with Scer\GAL4da.PU
    lethal, with Scer\GAL4en-e16E, mir-8jk22
    lethal, with Scer\GAL4en-e16E, mir-8UAS.cKa
    lethal, with Scer\GAL4pnr-MD237, mir-8jk22
    lethal, with Scer\GAL4pnr-MD237, mir-8UAS.cKa
    lethal | male, with Scer\GAL4da.G32
    lethal | recessive
    lethal - all die before end of pupal stage, with Scer\GAL4ptc-559.1
    lethal - all die during embryonic stage, with Scer\GAL4how-24B
    lethal - all die during embryonic stage, with Scer\GAL4how-24B, mir-8UAS.cLa
    lethal - all die during embryonic stage, with Scer\GAL4how-24B, mir-8Δ2
    partially lethal, with Df(2R)mir-8Δ3
    partially lethal (with Df(2R)mir-8Δ3)
    partially lethal (with mir-8Δ1)
    partially lethal (with mir-8Δ2)
    partially lethal | temperature conditional
    partially lethal - majority die, with Scer\GAL4NP5247
    partially lethal - majority die | pharate adult stage | temperature conditional
    partially lethal - majority die | pupal stage | temperature conditional
    partially lethal - majority live, with Scer\GAL4Tub.PU
    viable
    viable, with Scer\GAL4Act5C.PU
    viable | male, with Scer\GAL4Act5C.PI
    Sterility
    Allele
    female fertile (with Df(2R)BSC382)
    male fertile (with Df(2R)BSC382)
    Other Phenotypes
    Allele
    abnormal developmental rate
    abnormal locomotor behavior | adult stage (with Df(2R)mir-8Δ3)
    abnormal neuroanatomy
    abnormal neuroanatomy, with Scer\GAL4αTub84B.PL
    abnormal neuroanatomy | adult stage, with Scer\GAL4repo
    abnormal neuroanatomy | embryonic stage, with Scer\GAL4elav.PU
    abnormal neuroanatomy | embryonic stage, with Scer\GAL4how-24B
    abnormal neuroanatomy | embryonic stage | semidominant
    abnormal neuroanatomy | second instar larval stage
    abnormal neuroanatomy | third instar larval stage
    abnormal neuroanatomy | third instar larval stage, with Scer\GAL4moody.PS
    abnormal neuroanatomy | third instar larval stage, with Scer\GAL4NP5247
    abnormal neuroanatomy | third instar larval stage, with Scer\GAL4repo
    abnormal neurophysiology | third instar larval stage
    abnormal size (with Df(2R)mir-8Δ3)
    abnormal size | adult stage
    decreased body size
    decreased body size | adult stage
    decreased cell number
    decreased size | adult stage
    decreased size | adult stage, with Scer\GAL4Bx-MS1096
    increased cell death | first instar larval stage, with Scer\GAL4ey.PH
    increased cell death | third instar larval stage, with Scer\GAL4ey.PH
    increased cell death | third instar larval stage (with Df(2R)mir-8Δ3)
    increased cell number | adult stage, with Scer\GAL4Mef2.PR
    increased cell number | adult stage, with Scer\GAL4repo
    increased cell size | cell autonomous | somatic clone | third instar larval stage, with Scer\GAL4NP5247
    increased cell size | third instar larval stage, with Scer\GAL4moody.PS
    increased cell size | third instar larval stage, with Scer\GAL4repo
    long lived | male (with Df(2R)BSC382)
    short lived | temperature conditional
    some die during embryonic stage, with Scer\GAL4how-24B
    some die during embryonic stage, with Scer\GAL4how-24B, mir-8UAS.cLa
    some die during embryonic stage, with Scer\GAL4how-24B, mir-8Δ2
    some die during P-stage, with Df(2R)mir-8Δ3
    some die during P-stage (with Df(2R)mir-8Δ3)
    some die during P-stage (with mir-8Δ1)
    some die during P-stage (with mir-8Δ2)
    some die during pupal stage, with Scer\GAL4ptc-559.1
    visible, with Scer\GAL4Bx-MS1096
    visible, with Scer\GAL4ey.PH
    visible, with Scer\GAL4ptc-559.1
    visible, with Scer\GAL4sd-SG29.1
    visible, with Scer\GAL4vg.PU
    visible, with Scer\GAL4αTub84B.PL
    visible, with Scer\GAL4αTub84B.PL, mir-8sponge.UAS.EGFP
    visible, with Scer\GAL4αTub84B.PL, mir-8Δ2
    visible (with Df(2R)mir-8Δ3)
    visible | adult stage
    visible | adult stage, with mir-8jk22
    visible | adult stage, with mir-8Δ1
    visible | adult stage, with Scer\GAL41151
    visible | adult stage, with Scer\GAL4Bx-MS1096
    visible | adult stage, with Scer\GAL4Dll.PU
    visible | adult stage, with Scer\GAL4GMR.PF
    visible | adult stage (with Df(2R)ED2747)
    visible | adult stage | female limited
    visible | female limited, with Scer\GAL4pnr-MD237
    Phenotype manifest in
    Allele
    A1-7 ventral longitudinal muscle cell | embryonic stage
    A1-7 ventral oblique muscle cell | embryonic stage
    adult abdominal segment 6
    adult abdominal segment 6 | female limited, with Df(2R)ED2747
    adult abdominal segment 6 | female limited, with mir-8jk22
    adult abdominal segment 6 | female limited, with mir-8Δ1
    adult abdominal segment 6 | female limited, with Scer\GAL4pnr-MD237
    adult abdominal segment 6 | female limited (with Df(2R)ED2747)
    adult brain, with Scer\GAL4repo
    anterior crossvein, with Scer\GAL4Bx-MS1096
    axon | embryonic stage
    axon | embryonic stage, with Scer\GAL4elav.PU
    axon | embryonic stage, with Scer\GAL4how-24B
    CNS glial cell | adult stage | increased number, with Scer\GAL4repo
    dorsal-ventral compartment boundary of the eye disc | third instar larval stage, with Scer\GAL4ey.PH
    embryonic/larval neuromuscular junction | embryonic stage
    embryonic/larval neuromuscular junction | embryonic stage, with Scer\GAL4elav.PU
    embryonic/larval neuromuscular junction | embryonic stage, with Scer\GAL4how-24B
    embryonic/larval neuromuscular junction | second instar larval stage
    embryonic/larval neuromuscular junction | third instar larval stage
    embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4αTub84B.PL
    embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4αTub84B.PL, mir-8sponge.UAS.EGFP
    embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4αTub84B.PL, mir-8Δ2
    eye, with Scer\GAL4GMR.PF
    eye disc ventral compartment | first instar larval stage
    eye equator, with Scer\GAL4ey.PH
    eye ventral compartment, with Scer\GAL4ey.PH
    fat body | somatic clone
    first posterior wing cell, with Scer\GAL4ptc-559.1
    indirect flight muscle cell | adult stage, with Scer\GAL4GMR35H09
    larval brain (with Df(2R)mir-8Δ3)
    larval brain | third instar larval stage
    larval brain | third instar larval stage, with Scer\GAL4moody.PS
    larval brain | third instar larval stage, with Scer\GAL4NP5247
    larval brain | third instar larval stage, with Scer\GAL4repo
    larval glial cell | third instar larval stage, with Scer\GAL4moody.PS
    larval glial cell | third instar larval stage, with Scer\GAL4repo
    larval intersegmental nerve branch ISNb of A1-7 | embryonic stage
    larval intersegmental nerve branch ISNb of A1-7 | embryonic stage, with Scer\GAL4elav.PU
    larval intersegmental nerve branch ISNb of A1-7 | embryonic stage, with Scer\GAL4how-24B
    larval neuroblast | ectopic | third instar larval stage
    larval neuroblast | ectopic | third instar larval stage, with Scer\GAL4NP5247
    larval VL3/4 motor neuron | embryonic stage
    leg
    leg (with Df(2R)mir-8Δ3)
    leg (with mir-8Δ1)
    leg (with mir-8Δ2)
    mesothoracic leg
    metathoracic leg
    metathoracic leg, with Scer\GAL4Dll.PU
    metathoracic leg, with Scer\GAL4Tub.PU
    metathoracic leg, with Scer\GAL4αTub84B.PL
    metathoracic leg, with Scer\GAL4αTub84B.PL, mir-8sponge.UAS.EGFP
    metathoracic leg, with Scer\GAL4αTub84B.PL, mir-8Δ2
    muscle cell | adult stage, with Scer\GAL4Mef2.PR
    neuromuscular junction, with Scer\GAL4futsch-C380
    NMJ bouton, with Scer\GAL4futsch-C380
    NMJ bouton, with Scer\GAL4αTub84B.PL
    NMJ bouton, with Scer\GAL4αTub84B.PL, mir-8sponge.UAS.EGFP
    NMJ bouton, with Scer\GAL4αTub84B.PL, mir-8Δ2
    NMJ bouton | increased number | larval stage (with mir-8Δ1)
    NMJ bouton | increased number | larval stage (with mir-8Δ2)
    NMJ bouton | larval stage
    NMJ bouton | larval stage | increased number, with Scer\GAL4futsch-C380
    nucleus | adult stage, with Scer\GAL4GMR35H09
    subperineurial glial sheath
    subsynaptic reticulum | third instar larval stage
    type Ib terminal bouton | second instar larval stage
    type Ib terminal bouton | third instar larval stage
    ventral denticle belt, with Scer\GAL4hry.PU
    wing
    wing, with Scer\GAL41151
    wing, with Scer\GAL4Bx-MS1096
    wing, with Scer\GAL4sd-SG29.1
    wing, with Scer\GAL4vg.PU
    wing (with Df(2R)mir-8Δ3)
    wing (with mir-8Δ1)
    wing (with mir-8Δ2)
    wing margin, with Scer\GAL4sd-SG29.1
    wing vein, with Scer\GAL4sd-SG29.1
    wing vein | ectopic (with Df(2R)mir-8Δ3)
    wing vein L5, with Scer\GAL4Bx-MS1096
    Orthologs
    Downloads
    Download All DIOPT Orthologs
    Human Orthologs (via DIOPT v9.1)
    Species\Gene Symbol
    Score
    Best Score
    Best Reverse Score
    Source
    Compara
    Domainoid
    eggNOG
    Hieranoid
    Homologene
    Inparanoid
    OMA
    OrthoDB
    OrthoFinder
    OrthoInspector
    orthoMCL
    Panther
    Phylome
    SonicParanoid
    Alignment
    Complementation?
    Transgene?
    Homo sapiens (Human) (3)
    Hsap\MIR200A
    1 of 14
    Yes
    Yes
    Hsap\MIR200B
    1 of 14
    Yes
    Yes
    Hsap\MIR200C
    1 of 14
    Yes
    Yes
     
    1  
    Model Organism Orthologs (via DIOPT v9.1)
    Species\Gene Symbol
    Score
    Best Score
    Best Reverse Score
    Source
    Compara
    Domainoid
    eggNOG
    Hieranoid
    Homologene
    Inparanoid
    OMA
    OrthoDB
    OrthoFinder
    OrthoInspector
    orthoMCL
    Panther
    Phylome
    SonicParanoid
    Alignment
    Complementation?
    Transgene?
    Rattus norvegicus (Norway rat) (5)
    Rnor\Mir141
    1 of 14
    Yes
    Yes
    Rnor\Mir200b
    1 of 14
    Yes
    Yes
    Rnor\Mir429
    1 of 14
    Yes
    Yes
    Rnor\Mir3548
    1 of 14
    Yes
    Yes
    Rnor\Mir3575
    1 of 14
    Yes
    Yes
    Mus musculus (laboratory mouse) (5)
    Mmus\Mir141
    1 of 14
    Yes
    Yes
    Mmus\Mir200a
    1 of 14
    Yes
    Yes
    Mmus\Mir200b
    1 of 14
    Yes
    Yes
    Mmus\Mir200c
    1 of 14
    Yes
    Yes
    Mmus\Mir429
    1 of 14
    Yes
    Yes
    Xenopus tropicalis (Western clawed frog) (0)
    Danio rerio (Zebrafish) (6)
    Drer\mir141
    1 of 14
    Yes
    Yes
    Drer\mir200a
    1 of 14
    Yes
    Yes
    Drer\mir200b
    1 of 14
    Yes
    Yes
    Drer\mir200c
    1 of 14
    Yes
    Yes
    Drer\mir429b
    1 of 14
    Yes
    Yes
    Drer\mir429
    1 of 14
    Yes
    Yes
    Caenorhabditis elegans (Nematode, roundworm) (0)
    Anopheles gambiae (African malaria mosquito) (0)
    Arabidopsis thaliana (thale-cress) (0)
    Saccharomyces cerevisiae (Brewer's yeast) (0)
    Schizosaccharomyces pombe (Fission yeast) (0)
    Escherichia coli (enterobacterium) (0)
    Other Organism Orthologs (via OrthoDB)
    Data provided directly from OrthoDB:mir-8. Refer to their site for version information.
    Paralogs
    Downloads
    Download All DIOPT Paralogs
    Paralogs (via DIOPT v9.1)
    Human Disease Associations
    FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 1 )
    Allele
    Disease
    Evidence
    References
    mir-8UAS.cUa
    model of  cancer
    CEA with EgfrUAS.cBa
    (Eichenlaub et al., 2016)
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 3 )
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Dmel gene
    Ortholog showing functional complementation
    Supporting References
    mir-8
    Hsap\MIR200C
    (Hyun et al., 2009)
    Interactions
    Summary of Physical Interactions
    Interaction Browsers
    View in FlyBase Interactions Browser   View in MIST tool (external link)
    Please see the Physical Interaction reports below for full details
    RNA-protein
    Physical Interaction
    Assay
    References
    mir-8 - AGO1
    anti bait coimmunoprecipitation, northern blot, pull down, western blot
    (Kakumani et al., 2020, Lim et al., 2020)
    mir-8 - AGO2
    anti tag coimmunoprecipitation, northern blot
    (Abe et al., 2014)
    mir-8 - Dcr-1
    enzymatic study, autoradiography
    (Tsutsumi et al., 2011)
    mir-8 - Fmr1
    anti bait coimmunoprecipitation, quantitative reverse transcription pcr
    (Yang et al., 2009)
    mir-8 - His1
    anti tag coimmunoprecipitation, quantitative reverse transcription pcr
    (Liu et al., 2016)
    mir-8 - Rox8
    anti tag coimmunoprecipitation, quantitative reverse transcription pcr
    (Guo et al., 2020)
    mir-8 - SmD1
    anti bait coimmunoprecipitation, primer specific pcr
    (Xiong et al., 2015)
    mir-8 - SmD2
    anti tag coimmunoprecipitation, primer specific pcr
    (Xiong et al., 2015)
    mir-8 - Unr
    pull down, western blot
    (Kakumani et al., 2020)
    mir-8 - loqs
    fluorescence polarization spectroscopy, predetermined participant
    (Tants et al., 2017)
    RNA-RNA
    Physical Interaction
    Assay
    References
    mir-8 - ATP6AP2
    luminiscence technology, quantitative reverse transcription pcr
    (Lim et al., 2020)
    mir-8 - Adgf-A
    quantitative reverse transcription pcr
    (Lee et al., 2015)
    mir-8 - Adgf-B
    quantitative reverse transcription pcr
    (Lee et al., 2015)
    mir-8 - CG13060
    quantitative reverse transcription pcr
    (Karres et al., 2007)
    mir-8 - CG32767
    luminiscence technology, necessary binding region
    (Kennell et al., 2008)
    mir-8 - CG8420
    quantitative reverse transcription pcr
    (Karres et al., 2007)
    mir-8 - Ced-12
    luminiscence technology
    (Hyun et al., 2009)
    mir-8 - Cpr56F
    quantitative reverse transcription pcr
    (Karres et al., 2007)
    mir-8 - Gug
    quantitative reverse transcription pcr, luminiscence technology, necessary binding region
    (Karres et al., 2007)
    mir-8 - Idgf1
    quantitative reverse transcription pcr
    (Lee et al., 2015)
    mir-8 - Idgf2
    quantitative reverse transcription pcr
    (Lee et al., 2015)
    mir-8 - Idgf3
    quantitative reverse transcription pcr
    (Lee et al., 2015)
    mir-8 - Idgf6
    quantitative reverse transcription pcr
    (Lee et al., 2015)
    mir-8 - Ilp6
    quantitative reverse transcription pcr
    (Lee et al., 2015)
    mir-8 - ImpL2
    quantitative reverse transcription pcr
    (Lee et al., 2015)
    mir-8 - Lap1
    luminiscence technology
    (Hyun et al., 2009)
    mir-8 - Lar
    luminiscence technology
    (Nesler et al., 2013, Hyun et al., 2009)
    mir-8 - Ser
    luminiscence technology, necessary binding region, immunohistochemistry, fluorescence technology
    (Vallejo et al., 2011)
    mir-8 - Tl
    quantitative reverse transcription pcr, luminiscence technology
    (Lee and Hyun, 2014)
    mir-8 - Vha100-2
    luminiscence technology, quantitative reverse transcription pcr
    (Lim et al., 2020)
    mir-8 - Vha26
    western blot, luminiscence technology, quantitative reverse transcription pcr
    (Lim et al., 2020)
    mir-8 - Vha55
    luminiscence technology, western blot, quantitative reverse transcription pcr
    (Lim et al., 2020)
    mir-8 - VhaAC45
    quantitative reverse transcription pcr, luminiscence technology
    (Lim et al., 2020)
    mir-8 - Wdr37
    luminiscence technology
    (Hyun et al., 2009)
    mir-8 - caly
    luminiscence technology
    (Hyun et al., 2009)
    mir-8 - dbo
    luminiscence technology
    (Hyun et al., 2009)
    mir-8 - dl
    quantitative reverse transcription pcr, luminiscence technology
    (Lee and Hyun, 2014)
    mir-8 - ena
    immunohistochemistry, fluorescence technology, necessary binding region
    (Loya et al., 2014)
    mir-8 - pan
    western blot
    (Kennell et al., 2008)
    mir-8 - pnut
    immunohistochemistry
    (Eichenlaub et al., 2016)
    mir-8 - sd
    fluorescence technology
    (Sander et al., 2018)
    mir-8 - spi
    quantitative reverse transcription pcr, fluorescence technology, luminiscence technology, necessary binding region
    (Morante et al., 2013)
    mir-8 - ush
    luminiscence technology, western blot, quantitative reverse transcription pcr
    (Lee and Hyun, 2014, Hyun et al., 2009)
    mir-8 - wg
    luminiscence technology
    (Nesler et al., 2013)
    mir-8 - wls
    luminiscence technology, necessary binding region, immunohistochemistry
    (Kennell et al., 2008)
    mir-8 - yki
    fluorescence technology
    (Sander et al., 2018)
    Summary of Genetic Interactions
    Interaction Browsers
    View in FlyBase Interactions Browser   View in MIST tool (external link)
    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    mir-8
    suppressible
    ena
    (Loya et al., 2009)
    mir-8
    suppressible
    Gug
    (Karres et al., 2007)
    mir-8
    suppressible
    Nrg
    (Lu et al., 2014)
    mir-8
    enhanceable
    Ser
    (Vallejo et al., 2011)
    mir-8
    suppressible
    Ser
    (Vallejo et al., 2011)
    mir-8
    enhanceable
    spi
    (Morante et al., 2013)
    mir-8
    suppressible
    spi
    (Morante et al., 2013)
    mir-8
    suppressible
    ush
    (Hyun et al., 2009)
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    arm
    suppressible
    mir-8
    (Kennell et al., 2008)
    Delta|lola|psq
    suppressible
    mir-8
    (Vallejo et al., 2011)
    dup
    suppressible
    mir-8
    (Morante et al., 2013)
    Pi3K92E|btl::Egfr
    suppressible
    mir-8
    (Jarabo et al., 2021)
    External Data
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    MIST (genetic) - An integrated Molecular Interaction Database
    Pathways
    Class of Gene
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2R
    Recombination map
    2-80
    Cytogenetic map
    53D11-53D11
    Sequence location
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    53D11-53D11
    Limits computationally determined from genome sequence between P{EP}BEST:SD02913EP2148&P{lacW}Dekk09907 and P{lacW}CG6426k10209&P{lacW}vegk07202
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    Experimentally Determined Recombination Data
    Location

    2-80

    (FlyBase, 2016)

    2-84.5

    (Comeron et al., 2012)
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (5)
    Genomic Clones (10)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (0)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
      Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

          cDNA Clones, End Sequenced (ESTs)
          BDGP DGC clones
            Other clones
              RNAi and Array Information
              Linkouts
              Antibody Information
              Laboratory Generated Antibodies
               
              Commercially Available Antibodies
               
              Cell Line Information
              Publicly Available Cell Lines
               
                Other Stable Cell Lines
                 
                Other Comments

                Gug is a direct target of mir-8.

                (Karres et al., 2007)

                New annotation (CR33562) in release 4.1 of the genome annotation.

                (FlyBase Genome Annotators, 2005)

                New annotation (CR33018) in release 3 of the genome annotation.

                (FlyBase Genome Annotators, 2002-2003)
                Relationship to Other Genes
                Source for database merge of
                Additional comments

                The annotation for mir-8 has been changed in release 5.31 of the genome annotation, so that instead of representing a mature miRNA it now represents the precursor stem loop pre-miRNA. The symbol of the annotation has been changed from CR33018 to CR42988 to reflect this change.

                (FlyBase Genome Annotators, 2010)
                Nomenclature History
                Source for database identify of
                Nomenclature comments
                Etymology
                Synonyms and Secondary IDs (18)
                Reported As
                Symbol Synonym
                8
                (Han et al., 2011)
                8 mir
                (Ronshaugen and Levine, 2004)
                CR33018
                CR33562
                (Grieder et al., 2007, Molnar et al., 2006, FlyBase Genome Annotators, 2005)
                CR42988
                (FlyBase Genome Annotators, 2010)
                MIR-8
                (Ylla et al., 2016)
                MiR-8
                (Yang et al., 2009)
                dme-miR-8
                (Volin et al., 2018, Chandra et al., 2017, Dhahbi et al., 2016, Lucas et al., 2015, Towler et al., 2015, O'Farrell et al., 2008)
                dme-miR-8-3p
                (Chandra et al., 2015)
                dme-mir-8
                (He et al., 2022, Mohammed et al., 2014, miRBase, 2013, Berezikov et al., 2011, Gesellchen and Boutros, 2004)
                miR-8
                (Chen et al., 2024, Jang et al., 2024, Veneti et al., 2024, Chan et al., 2023, Sharpe et al., 2023, Yang et al., 2023, Yun and Hyun, 2023, Almeida Machado Costa et al., 2022, Dozier et al., 2022, Lu and Chtarbanova, 2022, Boukhatmi, 2021, Montigny et al., 2021, Parra-Peralbo et al., 2021, Zhang et al., 2021, Galagali and Kim, 2020, Gerlach and Herranz, 2020, Guo et al., 2020, Jin et al., 2020, Kakumani et al., 2020, Soleimani et al., 2020, Song and Zhou, 2020, Toprak et al., 2020, Xia et al., 2020, Reichholf et al., 2019, Boukhatmi and Bray, 2018, Cosacak et al., 2018, Richardson and Portela, 2018, Sander et al., 2018, Ueda et al., 2018, Atilano et al., 2017, Chandra et al., 2017, Liu and Jin, 2017, Monsanto-Hearne et al., 2017, Picao-Osorio et al., 2017, Tants et al., 2017, Umegawachi et al., 2017, Bolin et al., 2016, Brookheart and Duncan, 2016, Liu et al., 2016, Rainey et al., 2016, Verrando et al., 2016, Yang et al., 2016, Fulga et al., 2015, Lee et al., 2015, Lucas et al., 2015, Suh et al., 2015, Xiong et al., 2015, Zhai et al., 2015, Abe et al., 2014, Barrio et al., 2014, Kong et al., 2014, Kozomara et al., 2014, Lee and Hyun, 2014, Loya et al., 2014, Lu et al., 2014, Esslinger et al., 2013, Lucas and Raikhel, 2013, Mezan et al., 2013, Nesler et al., 2013, Pancratov et al., 2013, Rubio et al., 2013, Choi and Hyun, 2012, Jin et al., 2012, Kennell et al., 2012, Behura et al., 2011, Dávalos et al., 2011, Lawlor et al., 2011, Liu et al., 2011, Tsutsumi et al., 2011, Yu et al., 2011, Büssing et al., 2010, Fernandez-Valverde et al., 2010, Czech et al., 2009, Dubruille et al., 2009, Kadener et al., 2009, Kadener et al., 2009, Lee et al., 2009, Li et al., 2009, Loya et al., 2009, Yu et al., 2009, Zhou et al., 2009, Huang et al., 2008, Ibáñez-Ventoso et al., 2008, Kennell and Cadigan, 2008, Kennell et al., 2008, Okamura et al., 2008, Yu et al., 2008, Behura, 2007, Horwich et al., 2007, Karres et al., 2007, Nurminsky, 2007, Reynolds, 2007, Shcherbata et al., 2007, Nguyen and Frasch, 2006, Rehwinkel et al., 2006, Vagin et al., 2006, Leaman, 2005, Saito et al., 2005, Stark et al., 2005, Boutla et al., 2003, Lai et al., 2003, Schwarz et al., 2003, Sempere et al., 2003, Lagos-Quintana et al., 2001, Lee et al., 2001)
                miR-8-3p
                (Seong et al., 2018)
                miR8
                (Gutiérrez-Martínez et al., 2020, Berdnik et al., 2008)
                mir-8
                (Narbonne-Reveau et al., 2025, Collins et al., 2024, Matsuura-Suzuki et al., 2024, Megaly et al., 2024, Örkenby et al., 2023, Barish et al., 2022, Guo et al., 2020, Laurichesse and Soler, 2020, Hanyu-Nakamura et al., 2019, Bohère et al., 2018, Lehmann, 2018, Mohammed and Lai, 2016.2.8, Sanfilippo et al., 2016, Antonello et al., 2015, Antonello et al., 2015, Chen et al., 2014, Wang et al., 2014, Yeh et al., 2014, Daneshvar et al., 2013, Kato and Slack, 2013, Morante et al., 2013, Bejarano et al., 2012, Bejarano et al., 2012, Schertel et al., 2012, Szuplewski et al., 2012, Enderle et al., 2011, Okamura et al., 2011, Qian et al., 2011, Smibert et al., 2011, Vallejo et al., 2011, FlyBase Genome Annotators, 2010, Schnall-Levin et al., 2010, Hyun et al., 2009, Martin et al., 2009, Okamura et al., 2009, Neumuller et al., 2008, Okamura et al., 2008, Biryukova et al., 2007, Liu et al., 2007, Park et al., 2007, Ruby et al., 2007)
                mir-8*
                (Aravin et al., 2003)
                mir-8S
                (Grieder et al., 2007)
                mir8S
                (Molnar et al., 2006)
                Name Synonyms
                miR-8
                (Westholm et al., 2014)
                mir-8
                mir-8 stem loop
                mir-8S
                Secondary FlyBase IDs
                • FBgn0046815
                • FBgn0082172
                • FBgn0262281
                Datasets (0)
                Study focus (0)
                Experimental Role
                Project
                Project Type
                Title
                Study result (0)
                Result
                Result Type
                Title
                External Crossreferences and Linkouts ( 18 )
                Sequence Crossreferences
                NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                RNAcentral - A comprehensive ncRNA sequence collection representing all ncRNA types from a broad range of organisms
                Other crossreferences
                DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                KEGG Genes - Molecular building blocks of life in the genomic space.
                MARRVEL_MODEL - MARRVEL (model organism gene)
                miRBase - A searchable database of published miRNA sequences and annotation.
                Rfam - A collection of RNA sequence families of structural RNAs including non-coding RNA genes as well as cis-regulatory elements
                Linkouts
                DroID - A comprehensive database of gene and protein interactions.
                FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
                MIST (genetic) - An integrated Molecular Interaction Database
                References (199)