FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
mir-8, spi
FlyBase ID
FBig0000104415
Interaction Network
Interactions Browser links
mir-8 network
spi network
Reported Interactions
FBrf0223187-1.miR-mRNA.LUC
Reference
Morante et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
mir-8 

mir-8

bait
mir-8 
spi 

Spitz

prey
spi 
spi 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
spi 
3-prime UTR
sufficient binding region
spi 
mir-8 target site
necessary binding region
CGACACACGCGCCAAGUAUUA
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

luciferase reporter assay

(Morante et al., 2013)

Source was S2 cell line; bait produced from transfected construct; prey produced from transfected reporter construct.

(Morante et al., 2013)
FBrf0223187-2.miR-mRNA.GFP
Reference
Morante et al., 2013
Description
physical association
Assay
Collection
Source/Stage
wing disc / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mir-8 

mir-8

bait
mir-8 
spi 

Spitz

prey
spi 
spi 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
spi 
3-prime UTR
sufficient binding region
spi 
mir-8 target site
necessary binding region
CGACACACGCGCCAAGUAUUA
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

GFP reporter assay

(Morante et al., 2013)

Source was larval wing discs of transgenic fly line; bait produced from transgenic construct; prey produced from transgenic reporter construct.

(Morante et al., 2013)
FBrf0223187-3.miR-mRNA.PCR
Reference
Morante et al., 2013
Description
physical association
Assay
Collection
Source/Stage
larval brain / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mir-8 

mir-8

bait
spi 

Spitz

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Mutation of mir-8 increases spi mRNA levels.

(Morante et al., 2013)

Source was larval brains of mutant fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Morante et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)