FlyBase Allele Report: Dmel\mir-8[UAS.cVa]

General Information

Symbol

Dmel\mir-8^UAS.cVa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Vallejo

FlyBase ID

FBal0265088

Feature type

allele

Associated gene

Dmel\mir-8

Associated Insertion(s)

Carried in Construct

P{UAS-mir-8.V}

Also Known As

UAS-mir-8

Key Links

Transgenic product class

wild_type

(Vallejo et al., 2011)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Vallejo et al., 2011)

Mutagen

in vitro construct

(Vallejo et al., 2011)

Progenitor genotype

Carried in construct

P{UAS-mir-8.V}

Cytology

Description

UAS regulatory sequences drive expression of mir-8.

(Vallejo et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

mir-8

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates cancer

modeled by lola^GS88A8, psq^GS88A8

(Vallejo et al., 2011)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Expressing mir-8^UAS.cVa only during adulthood under the combined control of Scer\GAL4^GMR35H09 and Gal80[ts] results in significantly fewer nuclei per indirect flight muscle in 10-days old adults, as compared to controls.

The few surviving adults expressing mir-8^UAS.cVa under the control of Scer\GAL4¹¹⁵¹ exhibit held-out wings.

(Boukhatmi and Bray, 2018)

Scer\GAL4^repo-mediated expression of mir-8^Scer\UAS.cVa results in a 41% reduction in the size of the underlying neuroepithelium and overall brain size of L3 larvae compared to wild type. This is coupled with accelerated progression of the proneural wave.

Overexpression of mir-8^Scer\UAS.cVa in all glia using Scer\GAL4^repo increases optic lobe-associated cortex glial nuclear size and processes: glial cells immediately overlying the optic lobe neuroepithelium increase their size by ~400% and the moody-positive glia on top of this layer increase their size by ~500%. Central brain cortex glia also increased their size. However, satellite glia, epithelial glia, marginal glia and medulla neuropil glia ("inner glia") do not change cell size. Additionally, the number of non-mir-8 surface-associated glial cells is reduced in this genotype.

FLP-out glial clones overexpressing mir-8^Scer\UAS.cVa via Scer\GAL4^mir-8-NP5247 only contain a single cell, suggesting they do not proliferate. These cells increase cell size autonomously.

Expression of mir-8^Scer\UAS.cVa in the endoreplicating subperineurial glia cells using Scer\GAL4^moody.PS results in overgrown glial cells, but the growth of the underlying neuroepithelium and the timing of neuroblast transition are not affected.

(Morante et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^repo, mir-8^UAS.cVa has abnormal neuroanatomy | third instar larval stage phenotype, enhanceable by Scer\GAL4^repo/spi^GD1779

(Morante et al., 2013)

Suppressed by

Statement

Reference

Scer\GAL4^repo, mir-8^UAS.cVa has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by spi^s.UAS.EGFP/Scer\GAL4^repo

(Morante et al., 2013)

Suppressor of

Statement

Reference

Scer\GAL4^repo/mir-8^UAS.cVa is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Scer\GAL4^repo, dup^NIG.8171R

(Morante et al., 2013)

Scer\GAL4^repo/mir-8^UAS.cVa is a suppressor of decreased cell size | third instar larval stage phenotype of Scer\GAL4^repo, dup^NIG.8171R

(Morante et al., 2013)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^repo, mir-8^UAS.cVa has larval brain | third instar larval stage phenotype, enhanceable by Scer\GAL4^repo/spi^GD1779

(Morante et al., 2013)

Suppressed by

Statement

Reference

Scer\GAL4^repo, mir-8^UAS.cVa has larval brain | third instar larval stage phenotype, suppressible by spi^s.UAS.EGFP/Scer\GAL4^repo

(Morante et al., 2013)

Suppressor of

Statement

Reference

Scer\GAL4^repo/mir-8^UAS.cVa is a suppressor of larval brain | third instar larval stage phenotype of Scer\GAL4^repo, dup^NIG.8171R

(Morante et al., 2013)

Scer\GAL4^repo/mir-8^UAS.cVa is a suppressor of larval glial cell | third instar larval stage phenotype of Scer\GAL4^repo, dup^NIG.8171R

(Morante et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

The alterations in glial size and sprouting caused by Scer\GAL4^repo-mediated expression of dup^NIG.8171R are fully reversed by co-expression of mir-8^Scer\UAS.cVa.

The larval neuroepithelium defects caused by Scer\GAL4^repo-mediated expression of mir-8^Scer\UAS.cVa are fully reverted by co-expression of spi^{s.Scer\UAS.T:Avic\GFP-EGFP} (which lacks the native spi 3' UTR), but are enhanced by co-expression of spi^GD1779.

Some Scer\GAL4^repo, mir-8^Scer\UAS.cVa, spi^GD1779 larval brains exhibit areas of cell death, seemingly necrotic (not apoptotic) cell death.

(Morante et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

mir-8^Scer\UAS.cVa

mir-8^UAS.cVa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Vallejo

Secondary FlyBase IDs

References (5)

Report Sections

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