FlyBase Allele Report: Dmel\spi[GD1779]

General Information

Symbol

Dmel\spi^GD1779

Species

D. melanogaster

Name

FlyBase ID

FBal0210660

Feature type

allele

Associated gene

Dmel\spi

Associated Insertion(s)

Carried in Construct

P{GD1779}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD1779}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD1779

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates intestinal cancer

modeled by Sox21a^JC1

(Chen et al., 2016)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

ganglion mother cell | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

larval brain | third instar larval stage, with Scer\GAL4^NP5247

(Morante et al., 2013)

larval brain | third instar larval stage, with Scer\GAL4^repo

(Morante et al., 2013)

Detailed Description

Statement

Reference

Expression of spi^GD1779 under the control of either Scer\GAL4^repo.PL or Scer\GAL4^elav.PU does not affect the axon wrapping by the wrapping glia in third instar larvae.

(Matzat et al., 2015)

Scer\GAL4^mir-8-NP5247-mediated expression of spi^GD1779 results in a dramatic reduction in neuroepithelial size in L3 larval brains. These effects are less severe if Scer\GAL4^repo is used, and non-existant if Scer\GAL4^moody.PS is used.

(Morante et al., 2013)

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in shorter neuroblast lineages (less daughter cells per neuroblast) in the larval brain compared to controls.

(Neumüller et al., 2011)

Adults expressing spi^GD1779 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1779

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Scer\GAL4^repo/spi^GD1779 is an enhancer of abnormal neuroanatomy | third instar larval stage phenotype of Scer\GAL4^repo, mir-8^UAS.cVa

(Morante et al., 2013)

Suppressor of

Statement

Reference

Scer\GAL4^Su(H).GBE/spi^GD1779 is a suppressor | partially of increased cell number | adult stage phenotype of Sox21a^JC1

(Chen et al., 2016)

Scer\GAL4^elav-C155/spi^GD1779 is a suppressor | partially of abnormal sleep | recessive phenotype of rho-5^KO1

(Zettl et al., 2011)

Phenotype Manifest In

Enhancer of

Statement

Reference

Scer\GAL4^repo/spi^GD1779 is an enhancer of larval brain | third instar larval stage phenotype of Scer\GAL4^repo, mir-8^UAS.cVa

(Morante et al., 2013)

Suppressor of

Statement

Reference

Scer\GAL4^Su(H).GBE/spi^GD1779 is a suppressor | partially of adult midgut phenotype of Sox21a^JC1

(Chen et al., 2016)

Scer\GAL4^Su(H).GBE/spi^GD1779 is a suppressor | partially of intestinal stem cell phenotype of Sox21a^JC1

(Chen et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of spi^GD1779 under the control of Scer\GAL4^Su(H).GBE partially rescues the intestinal stem cell overproliferation phenotype in Sox21a^JC1/Sox21a^JC1 mutant intestines.

(Chen et al., 2016)

The larval neuroepithelium defects caused by Scer\GAL4^repo-mediated expression of mir-8^Scer\UAS.cVa are enhanced by co-expression of spi^GD1779.

Some Scer\GAL4^repo, mir-8^Scer\UAS.cVa, spi^GD1779 larval brains exhibit areas of cell death, seemingly necrotic (not apoptotic) cell death.

(Morante et al., 2013)

Expression of spi^GD1779 under the control of Scer\GAL4^elav-C155 significantly suppresses the sleep defects seen in homozygous rho-5^KO1 mutants.

(Zettl et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)