FlyBase Allele Report: Dmel\Sox21a[JC1]

General Information

Symbol

Dmel\Sox21a^JC1

Species

D. melanogaster

Name

FlyBase ID

FBal0319972

Feature type

allele

Associated gene

Dmel\Sox21a

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

CRISPR/Cas9

Nature of the Allele

Allele class

Mutagen

CRISPR/Cas9

(Chen et al., 2016)

Progenitor genotype

Cytology

Description

2.15kb deletion that removes most of the Sox21a coding region.

(Chen et al., 2016)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3L:14,104,459..14,106,564 [-]

Comment:

Approximate endpoints (probably an underestimate) of ~2.15 kb deletion created by Cas9-mediated gene knock out. Estimated from guide RNA sequences.

(Chen et al., 2016)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of intestinal cancer

CEA

(Chen et al., 2016)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of intestinal cancer

is ameliorated by Sox21a^UAS.cCa

(Chen et al., 2016)

is ameliorated by spi^GD1779

(Chen et al., 2016)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

fertile

(Chen et al., 2016)

increased cell number | adult stage | progressive

(Chen et al., 2016)

increased occurrence of cell division | adult stage

(Chen et al., 2016)

neoplasia | adult stage | progressive

(Chen et al., 2016)

neoplasia | adult stage | somatic clone

(Chen et al., 2016)

viable

(Chen et al., 2016)

Phenotype Manifest In

adult midgut | progressive

(Chen et al., 2016)

adult midgut | somatic clone

(Chen et al., 2016)

enteroblast | progressive

(Chen et al., 2016)

enteroblast | somatic clone

(Chen et al., 2016)

intestinal stem cell | progressive

(Chen et al., 2016)

intestinal stem cell | somatic clone

(Chen et al., 2016)

Detailed Description

Statement

Reference

Sox21a^JC1/Sox21a^JC1 mutants are viable and fertile. In newly eclosed flies, general gut morphology and cellular make up of the monolayer midgut epithelium is largely normal. As flies become older, tumors are frequently observed in both the anterior and posterior midgut, and both tumor incidence and size increase with age. Tumors are mainly composed of intestinal stem cells and enteroblasts.

The intestinal stem cells, but not enteroblasts, of Sox21a^JC1/Sox21a^JC1 mutant tumors are significantly increased in mitotic activity, as compared with wild type intestinal cells.

Sox21a^JC1/Sox21a^JC1 mutant clones give rise to intestinal tumors mainly composed of intestinal stem cells and enteroblasts.

(Chen et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Sox21a^JC1 has increased cell number | adult stage phenotype, suppressible | partially by Scer\GAL4^Su(H).GBE/spi^GD1779

(Chen et al., 2016)

Phenotype Manifest In

Suppressed by

Statement

Reference

Sox21a^JC1 has adult midgut phenotype, suppressible | partially by Scer\GAL4^Su(H).GBE/spi^GD1779

(Chen et al., 2016)

Sox21a^JC1 has intestinal stem cell phenotype, suppressible | partially by Scer\GAL4^Su(H).GBE/spi^GD1779

(Chen et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of spi^GD1779 under the control of Scer\GAL4^Su(H).GBE partially rescues the intestinal stem cell overproliferation phenotype in Sox21a^JC1/Sox21a^JC1 mutant intestines.

(Chen et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Sox21a^JC1 is partially rescued by Scer\GAL4^Su(H).GBE/Sox21a^UAS.cCa

(Chen et al., 2016)

Not rescued by

Sox21a^JC1 is not rescued by Scer\GAL4^{Delta-05151-G}/Sox21a^UAS.cCa

(Chen et al., 2016)

Comments

Expression of Sox21a^Scer\UAS.cCa under the control of Scer\GAL4^Su(H).GBE (along with GAL80ts to temporally control expression), but not Scer\GAL4^Dl-05151-G, largely suppresses tumor formation in Sox21a^JC1/Sox21a^JC1 mutants.

Expression of Sox21a^Scer\UAS.cCa under the control of Scer\GAL4^Su(H).GBE rescues the intestinal stem cell overproliferation phenotype in Sox21a^JC1/Sox21a^JC1 mutant intestines.

(Chen et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Sox21a^JC1

(Chen et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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