FlyBase Allele Report: Dmel\spi[s.UAS.EGFP]

General Information

Symbol

Dmel\spi^s.UAS.EGFP

Species

D. melanogaster

Name

FlyBase ID

FBal0278149

Feature type

allele

Associated gene

Dmel\spi

Associated Insertion(s)

Carried in Construct

P{UAS-spi.s.GFP}

Also Known As

UAS-cSpi-GFP, UAS-sSpi-GFP

Key Links

Transgenic product class

stop_gained

(Schlesinger et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

stop_gained

(Schlesinger et al., 2004)

Mutagen

in vitro construct

(Schlesinger et al., 2004, Tsruya et al., 2002)

Progenitor genotype

Carried in construct

P{UAS-spi.s.GFP}

Cytology

Description

UASt regulatory sequences drive expression of a cleaved form of spi (a stop codon has been introduced after P128, prior to the transmembrane domain), which is tagged after residue T54 with EGFP.

(Schlesinger et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

spi

Tagged with

EGFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal learning | larval stage | RU486 conditional, with Scer\GAL4^{elav.Switch.PO}

(Rahn et al., 2013)

abnormal neuroanatomy | third instar larval stage | heat sensitive, with Scer\GAL4^NP5247, Scer\GAL80^ts.αTub84B

(Morante et al., 2013)

increased cell number | third instar larval stage, with Scer\GAL4^elav-C155

(Kim et al., 2017)

increased cell number | third instar larval stage, with Scer\GAL4^GMR30H02

(Kim et al., 2017)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of spi^{s.Scer\UAS.T:Avic\GFP-EGFP} under the control of either Scer\GAL4^GMR30H02 or Scer\GAL4^elav-C155 results in significantly increased concentration of circulating hemocytes in third instar larvae, as compared to controls.

(Kim et al., 2017)

Expression of spi^{s.Scer\UAS.T:Avic\GFP-EGFP} via Scer\GAL4^mir-8-NP5247 at the early L3 stage (by inclusion of Scer\GAL80^ts.αTub84B and maintenance at the permissive temperature until that point) results in a dramatic expansion of the neuroepithelium of L3 larvae, coupled with ectopic and precocious neuroblasts.

(Morante et al., 2013)

Expression of spi^{s.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^{elav.Switch.PO} for 24 hours during larval stages (using mifepristone to control the timing of expression) results in significantly reduced olfactory learning ability in larvae. These larvae show normal gustatory preference responses to fructose and to quinine and show normal olfactory responses to amyl acetate and to 1-octanol.

(Rahn et al., 2013)

Expression of spi^{s.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^{neur-GAL4-A101} in sensory organs of the leg results in induction of bract cell fate in all surrounding cells, although in some cases, at a greater distance on the proximal side of the leg.

(Peng et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

spi^s.UAS.EGFP/Scer\GAL4^repo is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Scer\GAL4^repo, mir-8^UAS.cVa

(Morante et al., 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^Act5C.PP, rho^UAS.cGa, spi^s.UAS.EGFP has eye photoreceptor cell | somatic clone phenotype, suppressible by rasp^T392/rasp^T802

(Miura et al., 2006)

Suppressor of

Statement

Reference

spi^s.UAS.EGFP/Scer\GAL4^repo is a suppressor of larval brain | third instar larval stage phenotype of Scer\GAL4^repo, mir-8^UAS.cVa

(Morante et al., 2013)

Scer\GAL4^{neur-GAL4-A101}/spi^s.UAS.EGFP is a suppressor of bract | somatic clone phenotype of dia⁵

(Peng et al., 2012)

Other

Statement

Reference

Scer\GAL4^Act5C.PP, rho^UAS.cGa, spi^s.UAS.EGFP has eye photoreceptor cell | somatic clone phenotype

(Miura et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

The larval neuroepithelium defects caused by Scer\GAL4^repo-mediated expression of mir-8^Scer\UAS.cVa are fully reverted by co-expression of spi^{s.Scer\UAS.T:Avic\GFP-EGFP} (which lacks the native spi 3' UTR).

(Morante et al., 2013)

Expression of spi^{s.Scer\UAS.T:Avic\GFP-EGFP} in the sensory organ precursor lineage (using Scer\GAL4^{neur-GAL4-A101}) in large homozygous dia⁵ clones in the leg restores bract formation in the clones.

(Peng et al., 2012)

Somatic clones of Scer\GAL4^Act5C.PP cells in the eye disc in animals carrying rho^Scer\UAS.cGa and spi^{Scer\UAS.s.T:Avic\GFP} cause ectopic photoreceptor differentiation. This phenotype is completely suppressed by rasp^T392/rasp^T802.

(Miura et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

spi^{Scer\UAS.s.T:Avic\GFP}

spi^{c.Scer\UAS.T:Avic\GFP-EGFP}

spi^{s.Scer\UAS.T:Avic\GFP-EGFP}

spi^s.UAS.EGFP

Name Synonyms

Secondary FlyBase IDs

FBal0221767
FBal0129042

References (8)

Report Sections

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