FlyBase Allele Report: Dmel\dup[NIG.8171R]

General Information

Symbol

Dmel\dup^NIG.8171R

Species

D. melanogaster

Name

FlyBase ID

FBal0275882

Feature type

allele

Associated gene

Dmel\dup

Associated Insertion(s)

Carried in Construct

P{NIG.8171R}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Japanese National Institute of Genetics, 2012.5.21)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Japanese National Institute of Genetics, 2012.5.21)

Mutagen

in vitro construct

(Japanese National Institute of Genetics, 2012.5.21)

Progenitor genotype

Carried in construct

P{NIG.8171R}

Cytology

Description

UASt regulatory sequences drive expression of a 500bp inverted repeat.

(Japanese National Institute of Genetics, 2012.5.21)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-8171R

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^repo

(Morante et al., 2013)

decreased cell size, with Scer\GAL4^ptc-559.1

(Park and Asano, 2008)

decreased cell size | third instar larval stage, with Scer\GAL4^repo

(Morante et al., 2013)

Phenotype Manifest In

embryonic/larval salivary gland, with Scer\GAL4^ptc-559.1

(Park and Asano, 2008)

larval brain | third instar larval stage, with Scer\GAL4^repo

(Morante et al., 2013)

larval glial cell | third instar larval stage, with Scer\GAL4^repo

(Morante et al., 2013)

larval neuroblast | ectopic | third instar larval stage, with Scer\GAL4^repo

(Morante et al., 2013)

nucleus, with Scer\GAL4^ptc-559.1

(Park and Asano, 2008)

Detailed Description

Statement

Reference

Expression of dup^NIG.8171R in all glia using Scer\GAL4^repo reduces the cell number and size of surface glia in L3 larval brains. The only remaining surface glia display short processes. The underlying neuroepithelium in these brains is highly disorganised with intermingled, ectopic neuroepithelial-neuroblast transitions.

(Morante et al., 2013)

Down-regulation of dup through expression of dup^NIG.8171R under the control of Scer\GAL4^ptc-559.1 causes a dramatic decrease in nuclear size, cell size, and DNA content of salivary glands, whereas no detectable change is observed in fat bodies, where Scer\GAL4^ptc-559.1 does not drive expression.

(Park and Asano, 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^repo, dup^NIG.8171R has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Scer\GAL4^repo/mir-8^UAS.cVa

(Morante et al., 2013)

Scer\GAL4^repo, dup^NIG.8171R has decreased cell size | third instar larval stage phenotype, suppressible by Scer\GAL4^repo/mir-8^UAS.cVa

(Morante et al., 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^repo, dup^NIG.8171R has larval brain | third instar larval stage phenotype, suppressible by Scer\GAL4^repo/mir-8^UAS.cVa

(Morante et al., 2013)

Scer\GAL4^repo, dup^NIG.8171R has larval glial cell | third instar larval stage phenotype, suppressible by Scer\GAL4^repo/mir-8^UAS.cVa

(Morante et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

The alterations in glial size and sprouting caused by Scer\GAL4^repo-mediated expression of dup^NIG.8171R are fully reversed by co-expression of mir-8^Scer\UAS.cVa.

(Morante et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

NIG-Fly

8171R-2

P{NIG.8171R}2

8171R-3

P{NIG.8171R}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

dup^NIG.8171R

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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