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Nil, Z., Millán, R.H., Gerbich, T., Leal, P., Yu, Z., Saraf, A., Sardiu, M., Lange, J.J., Yi, K., Unruh, J., Slaughter, B., Si, K. (2019). Amyloid-like Assembly Activates a Phosphatase in the Developing Drosophila Embryo.  Cell 178(6): 1403--1420.e21.
FlyBase ID
FBrf0243353
Publication Type
Research paper
Abstract

Prion-like proteins can assume distinct conformational and physical states in the same cell. Sequence analysis suggests that prion-like proteins are prevalent in various species; however, it remains unclear what functional space they occupy in multicellular organisms. Here, we report the identification of a prion-like protein, Herzog (CG5830), through a multimodal screen in Drosophila melanogaster. Herzog functions as a membrane-associated phosphatase and controls embryonic patterning, likely being involved in TGF-β/BMP and FGF/EGF signaling pathways. Remarkably, monomeric Herzog is enzymatically inactive and becomes active upon amyloid-like assembly. The prion-like domain of Herzog is necessary for both its assembly and membrane targeting. Removal of the prion-like domain impairs activity, while restoring assembly on the membrane using a heterologous prion-like domain and membrane-targeting motif can restore phosphatase activity. This study provides an example of a prion-like domain that allows an enzyme to gain essential functionality via amyloid-like assembly to control animal development.

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PubMed Central ID
Related Publication(s)
Erratum

Amyloid-like Assembly Activates a Phosphatase in the Developing Drosophila Embryo.
Nil et al., 2019, Cell 179(3): 801 [FBrf0243774]

Note

Amyloid-directed phosphatase activation.
Strzyz, 2019, Nat. Rev. Mol. Cell Biol. 20(10): 571 [FBrf0245007]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Cell
    Title
    Cell
    Publication Year
    1974-
    ISBN/ISSN
    0092-8674
    Data From Reference
    Alleles (5)
    Genes (25)
    Physical Interactions (31)
    Cell Lines (1)
    Insertions (2)
    Experimental Tools (2)
    Transgenic Constructs (1)