FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
dl
FlyBase ID
FBig0000098198
Interaction Network
Interactions Browser links
dl network
Reported Interactions
FBrf0057556-1.coIP.WB
Reference
Govind et al., 1992
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dl 

dl

bait

fused to β-galactosidase

dl 

dl

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Govind et al., 1992)
FBrf0057556-2.coIP.WB
Reference
Govind et al., 1992
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dl 

dl

bait
dl 

dl

prey

fused to β-galactosidase

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from endogenous gene; prey produced from tagged transgenic construct.

(Govind et al., 1992)
FBrf0073424-1.XL.WB
Reference
Isoda and Nusslein-Volhard, 1994
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dl 

dl

self
dl 

dl

self
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins derived from wild-type embryo extract.

(Isoda and Nusslein-Volhard, 1994)

dl exists as a 190 kDa homodimer.

(Isoda and Nusslein-Volhard, 1994)
FBrf0086422-1.coIP.WB
Reference
Govind et al., 1996
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dl 

dl

bait

fused to β-galactosidase

dl 

dl

prey
dl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
dl 
N-terminal domain
sufficient binding region
aa 222-337

coordinates relative to dl-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Govind et al., 1996)
FBrf0099196-9.Y2H
Reference
Edwards et al., 1997
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dl 

Dorsal

bait

fused to LexA DNA-binding domain

dl 

Dorsal

prey

fused to B42 activation domain

dl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
dl 
Rel domain
sufficient binding region
aa 47-345
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Edwards et al., 1997)

dl self-associates.

(Edwards et al., 1997)

Two-hybrid system: yeast LexA-BD/B42-AD

(Edwards et al., 1997)
FBrf0243353-4.FRET.FM
Reference
Nil et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DRSC
Participants
Corresponds to
Reported as
Role
Note
dl 

Dl

fluorescence donor

CFP tag

dl 

Dl

fluorescence donor

CFP tag

dl 

Dl

fluorescence acceptor

YFP tag

dl 

Dl

fluorescence acceptor

YFP tag

dl 

Dl

fluorescence donor

CFP tag

dl 

Dl

fluorescence acceptor

YFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was live S2 cells; proteins produced from transfected constructs.

(Nil et al., 2019)
External Crossreferences and Linkouts ( 0 )
References (5)