FlyBase Gene Report: Dmel\Magi

General Information

Symbol

Dmel\Magi

Species

D. melanogaster

Name

Magi

Annotation Symbol

CG30388

Feature Type

protein_coding_gene

FlyBase ID

FBgn0034590

Gene Model Status

Current

Stock Availability

12 publicly available

Gene Summary

Magi (Magi) encodes a molecular scaffolding protein that is involved in adherens junction (AJ) organization. Magi is ubiquitously expressed and its product localizes at AJ with the Par polarity complex. [Date last reviewed: 2018-10-18] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Interactive Fly

Also Known As

ima

Key Links

Genomic Location

Cytogenetic map

57C2-57C3

Sequence location

2R:21,129,463..21,142,261 [-]

Recombination map

2-93

RefSeq locus

NT_033778 REGION:21129463..21142261

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (8 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables small GTPase binding

inferred from physical interaction with FLYBASE:Rala; FB:FBgn0015286

(Beller et al., 2002)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in adherens junction organization

inferred from mutant phenotype

(Zaessinger et al., 2015)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in signal transduction

inferred from biological aspect of ancestor with PANTHER:PTN000817101

(GO Reference Genome Project, 2011-)

Cellular Component (5 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

located_in adherens junction

inferred from direct assay

(Padash Barmchi et al., 2016)

located_in apicolateral plasma membrane

inferred from direct assay

(Padash Barmchi et al., 2016)

located_in muscle cell postsynaptic specialization

inferred from direct assay

(Jokhi et al., 2013)

located_in terminal bouton

inferred from direct assay

(Jokhi et al., 2013)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000817101

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Summary (Interactive Fly)

member of a family of proteins contains modular domains such as WW and PDZ domains necessary for scaffolding of membrane receptors and intracellular signaling components - adherens junction - forms a complex with polarity proteins Par3/Bazooka and aPKC

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Magi for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9W2L2)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.51

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Magi-RA

FBtr0071636

NM_137707

5003

1202

Magi-RB

FBtr0342873

NM_001299732

4706

1202

Magi-RC

FBtr0342874

NM_001299733

4475

1202

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Magi-PA

FBpp0071559

130.6

1202

6.49

FBpp0309675

130.6

1202

6.49

FBpp0309676

130.6

1202

6.49

Q9W2L2

NP_001286662

AHN56457

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1202 aa isoforms: Magi-PA, Magi-PB, Magi-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Magi using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.15

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 5 of adulthood | male -- day 7 of adulthood | male

spermatozoon

(Wasbrough et al., 2010)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in adherens junction

inferred from direct assay

(Padash Barmchi et al., 2016)

located_in apicolateral plasma membrane

inferred from direct assay

(Padash Barmchi et al., 2016)

located_in muscle cell postsynaptic specialization

inferred from direct assay

(Jokhi et al., 2013)

located_in terminal bouton

inferred from direct assay

(Jokhi et al., 2013)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Magi in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Magi

View exonic expression by tissue for Dmel\Magi

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37403/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0034590

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0034590

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0034590

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG30388

Flygut - An atlas of the Drosophila adult midgut

FBgn0034590

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 5 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 14 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Magi

Transgenic constructs containing regulatory region of Magi

Aberrations (Deficiencies and Duplications) ( 3 )

Inferred from experimentation ( 3 )

Gene disrupted in

Df(2R)Exel6072

(Ryder, 2004.4.26)

Df(2R)Exel6073

(Ryder, 2004.4.26)

Df(2R)Exel7167

(Ryder, 2004.4.26)

Inferred from location ( 6 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

Magi^bst

viable, with Scer\GAL4^Mef2.PR

Magi^GD10066

viable, with Scer\GAL4^pnr-MD237

Magi^GD10066

viable, with Scer\GAL4^tin.CΔ4

Magi^GD10066

Other Phenotypes

Allele

visible, with Scer\GAL4^da.G32

Magi^UAS.cBa

visible, with Scer\GAL4^elav-C155

Magi^UAS.cBa

visible, with Scer\GAL4^T80

Magi^UAS.cBa

visible | adult stage

Magi^ex214

visible | adult stage, with Scer\GAL4^GMR.PU

Magi^UAS.GFP

wild-type, with Scer\GAL4^en-e16E

Magi^UAS.cBa

Phenotype manifest in

Allele

adherens junction | pupal stage | somatic clone

Magi^ex214

eye, with Scer\GAL4^da.G32

Magi^UAS.cBa

eye, with Scer\GAL4^elav-C155

Magi^UAS.cBa

eye, with Scer\GAL4^T80

Magi^UAS.cBa

eye | adult stage

Magi^ex214

eye | adult stage, with Scer\GAL4^GMR.PU

Magi^UAS.GFP

eye disc | pupal stage | somatic clone

Magi^ex214

interommatidial bristle | adult stage

Magi^ex214

interommatidial bristle | adult stage (with Df(2R)Exel6072)

Magi^ex214

interommatidial cell | pharate adult stage, with Scer\GAL4^GMR.PU

Magi^UAS.GFP

interommatidial cell | pupal stage | increased number

Magi^ex214

interommatidial cell | pupal stage | increased number (with Df(2R)Exel6072)

Magi^ex214

ommatidium, with Scer\GAL4^da.G32

Magi^UAS.cBa

ommatidium, with Scer\GAL4^elav-C155

Magi^UAS.cBa

ommatidium, with Scer\GAL4^T80

Magi^UAS.cBa

scutellar bristle | increased number, with Scer\GAL4^da.G32

Magi^UAS.cBa

scutellar bristle | increased number, with Scer\GAL4^elav-C155

Magi^UAS.cBa

scutellar bristle | increased number, with Scer\GAL4^T80

Magi^UAS.cBa

slit diaphragm | decreased number | larval stage, with Scer\GAL4^sns.GCN

Magi^GD10066

wing blade

Magi^ex214

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (74)

Hsap\MAGI2

11 of 14

Yes

Hsap\MAGI3

11 of 14

Yes

Hsap\MAGI1

10 of 14

Yes

Hsap\MAGIX

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (47)

Rnor\Magi2

11 of 14

Yes

Rnor\Magi3

11 of 14

Yes

Rnor\Magi1

10 of 14

Yes

Rnor\Magix

3 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (55)

Mmus\Magi2

11 of 14

Yes

Mmus\Magi3

10 of 14

Yes

Mmus\Magi1

9 of 14

Yes

Mmus\Magix

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (31)

Xtro\magi3

9 of 13

Yes

Xtro\magi2

7 of 13

Yes

Xtro\magi1

6 of 13

Yes

5 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (53)

Drer\magi2a

11 of 14

Yes

Drer\LOC564220

10 of 14

Yes

Drer\magi1a

10 of 14

Yes

Drer\magi1b

10 of 14

Yes

Drer\magi2b

10 of 14

Yes

Drer\magi3a

10 of 14

Yes

Drer\magixa

4 of 14

Yes

Drer\LOC556872

3 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (16)

Cele\magi-1

10 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (29)

Agam\AgaP_AGAP010754

5 of 12

Yes

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (8)

Atha\AT2G30600

1 of 13

Yes

Atha\KAK

1 of 13

Yes

Atha\UPL1

1 of 13

Yes

Atha\UPL2

1 of 13

Yes

Atha\UPL4

1 of 13

Yes

Atha\UPL5

1 of 13

Yes

Atha\UPL6

1 of 13

Yes

Atha\UPL7

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (5)

Scer\HUL4

1 of 13

Yes

Scer\HUL5

1 of 13

Yes

Scer\RSP5

1 of 13

Yes

Scer\TOM1

1 of 13

Yes

Scer\UFD4

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (7)

Spom\mug30

1 of 12

Yes

Spom\ptr1

1 of 12

Yes

Spom\pub1

1 of 12

Yes

Spom\pub2

1 of 12

Yes

Spom\pub3

1 of 12

Yes

Spom\SPAC12B10.01c

1 of 12

Yes

Spom\SPAC167.07c

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Magi. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (26)

CG42788

2 of 13

sav

2 of 13

Smurf

2 of 13

Su(dx)

2 of 13

yki

2 of 13

baz

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

hyd

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

MAGI2; membrane associated guanylate kinase, WW and PDZ domain containing 2

model of nephrotic syndrome type 15

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

MAGI2; membrane associated guanylate kinase, WW and PDZ domain containing 2

11 of 14

606382

NEPHROTIC SYNDROME, TYPE 15; NPHS15

nephrotic syndrome type 15

MAGI3; membrane associated guanylate kinase, WW and PDZ domain containing 3

11 of 14

615943

MAGI1; membrane associated guanylate kinase, WW and PDZ domain containing 1

10 of 14

602625

MAGIX; MAGI family member, X-linked

3 of 14

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Magi - ASPP

anti tag coimmunoprecipitation, anti tag western blot

(Zaessinger et al., 2015)

Magi - Ack

anti tag coimmunoprecipitation, peptide massfingerprinting

(Schoenherr et al., 2012)

Magi - RASSF8

anti tag coimmunoprecipitation, anti tag western blot

(Zaessinger et al., 2015)

Magi - Rala

two hybrid, pull down, autoradiography

(Beller et al., 2002)

Magi - aPKC

proximity ligation assay, fluorescence microscopy, anti bait coimmunoprecipitation, western blot

(Padash Barmchi et al., 2016)

Magi - baz

anti bait coimmunoprecipitation, western blot, proximity ligation assay, fluorescence microscopy

(Padash Barmchi et al., 2016)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Magi

enhanceable

arm

(Zaessinger et al., 2015)

Magi

suppressible

RASSF8

(Zaessinger et al., 2015)

Magi

enhanceable

RASSF8

(Zaessinger et al., 2015)

Magi

enhanceable

sav

(Zaessinger et al., 2015)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

63040

DroID - A comprehensive database of gene and protein interactions.

FBgn0034590

MIST (genetic) - An integrated Molecular Interaction Database

37403

MIST (protein-protein) - An integrated Molecular Interaction Database

37403

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q8SX30

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-93

Cytogenetic map

57C2-57C3

Sequence location

2R:21,129,463..21,142,261 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

57C2-57C3

Limits computationally determined from genome sequence between P{PZ}king-tubby⁰²⁸²¹&P{lacW}Xbp1^k13803 and P{EP}CG4266^EP2258

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-93

(FlyBase, 2016)

2-100.3

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (12)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

22093

w¹¹¹⁸; P{EPg}Magi^HP25847

25792

y¹ v¹; P{TRiP.JF01798}attP2

59488

y¹ w^*; Mi{MIC}Magi^MI14279

35279

y¹ sc^* v¹ sev²¹; P{TRiP.GL00180}attP2

33411

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00291}attP2/TM3, Sb¹

NIG-Fly

15656R-1

P{NIG.15656R}1

15656R-2

P{NIG.15656R}2

GL00180

y¹ sc^* v¹ sev²¹; P{TRiP.GL00180}attP2

2RG-0422

y² VhaAC39-1^cho-1 v¹; P{U6-Magi.NIG.sgRNA}attP40/CyO

HMS00291

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00291}attP2/TM3, Sb¹

VDRC

v41735

w¹¹¹⁸; P{GD10066}v41735

v41736

w¹¹¹⁸; P{GD10066}v41736/CyO

Genomic Clones (22)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (16)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0034590

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0034590

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Padash Barmchi et al., 2016, Zaessinger et al., 2015, Jokhi et al., 2013)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Identification: Yeast-2-hybrid screen to identify Rala-interacting proteins.

(Beller et al., 2002)

Magi protein could play a role in coupling activin-dependent signaling to translational control and/or to the transport of RNAs.

(Beller et al., 2002)

Relationship to Other Genes

Source for database merge of

Source for merge of: Magi CG15656 CG4117

(Kuchinke and Thomas, 2002.3.5)

Additional comments

Annotations CG15656, CG4117 merged as CG30388 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Nomenclature History

Source for database identify of

Source for identity of: Magi Ima

(Beller, 2002.3.18)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

CG15656

CG30388

(National Institute of Genetics Fly Stocks, 2022-, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Perkins et al., 2009.1.15)

CG4117

D-MAGI

(Kuchinke et al., 2001)

Ima

(Beller et al., 2002)

ima

(Beller, 2002.3.18, Beller et al., 2002, Beller et al., 2002)

magi

(Maywald et al., 2022, Packard et al., 2015, Jokhi et al., 2013)

Name Synonyms

Magi

Secondary FlyBase IDs

FBgn0034591
FBgn0050388

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 48 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

37403

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9W2L2

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9W2L2

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37403/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0034590

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0034590

FlyMine - An integrated database for Drosophila genomics

FBgn0034590

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG30388

MARRVEL_MODEL - MARRVEL (model organism gene)

37403

Linkouts

BioGRID - A database of protein and genetic interactions.

63040

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0034590

DroID - A comprehensive database of gene and protein interactions.

FBgn0034590

DRSC - Results frm RNAi screens

FBgn0034590

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0034590

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0034590

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG30388

Flygut - An atlas of the Drosophila adult midgut

FBgn0034590

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC013622

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (genetic) - An integrated Molecular Interaction Database

37403

MIST (protein-protein) - An integrated Molecular Interaction Database

37403

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q8SX30

References (63)

The following external sites may use different assemblies or annotations than FlyBase.

0.15

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