Two-hybrid system: yeast LexA-BD/GAL4-AD
Bait Rala protein was mutated to be constitutively activated (G20V) and prevent the addition of the geranyl geranyl membrane targeting modifications (C198S).
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.
