FB2026_02 , released June 18, 2026

CV Term Report

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General Information

Term

protein complementation assay

ID (Ontology)

MI:0090 (Molecular Interactions)

Definition

In the protein-fragment complementation assay, the proteins of interest (\"Bait\" and \"Prey\") are covalently linked at the genetic level to incomplete fragments of a third protein (known as the \"reporter\") and are expressed in vivo, Interaction between the \"bait\" and the \"prey\" proteins brings the fragments of the \"reporter\" protein in close enough proximity to allow them to reform and become the functional reporter protein. Typically enzymes which confer resistance to antibiotics, such as Dihydrofolate reductase or Beta-lactamase, or proteins that give colorimetric or fluorescent signals are used. The Bait protein is generally the protein under study and the methods are readily adaptable to highthroughput mode.[ PubMed:11495741 ]

Also Known As

"complementation" ; "PCA"

Comment

Links to External Ontologies

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Records annotated with this term OR any of its CHILD TERMS

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Full annotation statements including this term (annotations to child terms are NOT included), and relevant FlyBase records

Full annotation statements	Relevant FlyBase reports
Full annotation statements	Interaction Groups
protein complementation assay (all annotations which use CV term, excluding "NOT" statements)

Spanning Tree (Parents/Children)

  interaction detection method
   |__experimental interaction detection
       |__protein complementation assay  5990 rec.
           |__adenylate cyclase complementation
           |__beta galactosidase complementation 1 rec.
           |__beta lactamase complementation
           |__bimolecular fluorescence complementation 599 rec.
           |__dihydrofolate reductase reconstruction 1 rec.
           |__mammalian protein protein interaction trap
           |__protein kinase A complementation
           |__reverse ras recruitment system
           |__split luciferase complementation 59 rec.
           |   |__split firefly luciferase complementation
           |   |__Split renilla luciferase complementation
           |__tox-r dimerization assay 1 rec.
           |__transcriptional complementation assay 5400 rec.
               |__lambda repressor two hybrid
               |__lex-a dimerization assay
               |__membrane yeast two hybrid(+) 4 rec.
               |__one hybrid 9 rec.
               |__reverse two hybrid
               |__three hybrid(+) 54 rec.
               |__two hybrid(+) 5363 rec.

Spanning Tree View Settings
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Relationships
Is a	experimental interaction detection
Part of
Synonyms & Secondary IDs
Synonyms
	"complementation" EXACT PSI-MI-short "PCA" EXACT PSI-MI-alternate
Secondary IDs

External Crossreferences & Linkouts