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Citation
Bilioni, A., Craig, G., Hill, C., McNeill, H. (2005). Iroquois transcription factors recognize a unique motif to mediate transcriptional repression in vivo.  Proc. Natl. Acad. Sci. U.S.A. 102(41): 14671--14676.
FlyBase ID
FBrf0191427
Publication Type
Research paper
Abstract

Iroquois transcription factors regulate diverse aspects of developmental patterning in all metazoans. Despite their widespread importance, the direct targets of the Iroquois are poorly understood. Here, we use in vitro site selection to define the DNA-binding preference of the Drosophila Iroquois Mirror. We use electrophoretic mobility shift assays to determine the critical nucleotides for Mirror binding and to show that this site is recognized by other Drosophila Iroquois transcription factors. This site also is recognized by vertebrate Iroquois transcription factors. Transgenic analysis demonstrates that Drosophila Iroquois proteins recognize this site in vivo to mediate transcriptional repression. We further show that Iroquois transcription factors form homodimers and heterodimers, suggesting that combinatorial binding may contribute to gene regulation by this family.

PubMed ID
PubMed Central ID
PMC1239941 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Proc. Natl. Acad. Sci. U.S.A.
    Title
    Proceedings of the National Academy of Sciences of the United States of America
    Publication Year
    1915-
    ISBN/ISSN
    0027-8424
    Data From Reference
    Alleles (3)
    Genes (3)
    Physical Interactions (2)
    Cell Lines (1)
    Insertions (1)
    Transgenic Constructs (2)