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General Information
Symbol
Tag:TAP
FlyBase ID
FBto0000299
Name
tandem affinity purification tag
Description
Description

The Tag:TAP sequence is a multiple component tag designed for tandem affinity purification and consists of a calmodulin binding peptide, a TEV protease recognition sequence and the Tag:proteinA purification sequence (PMID:10504710). When fused to the N- or C-terminal end of a protein of interest, a tandem affinity purification tag allows for rapid purification under native conditions of complexes, even when expressed at their natural level. Prior knowledge of complex composition or function is not required (PMID:11403571).

External Crossreferences and Linkouts
Related experimental tools
Transgenic Constructs
Tagged with the tool (90)
Transgenic construct(s)
Component allele
Reg. region
Encoded product / tool
Tagged with
Also carries
Stocks
Insertions ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
T:Zzzz\TAP
Tag:TAP
Name Synonyms
tandem affinity purification tag
Secondary FlyBase IDs
  • FBgn0042688
References (44)