FlyBase Gene Report: Dmel\Sfmbt

General Information

Symbol

Dmel\Sfmbt

Species

D. melanogaster

Name

Scm-related gene containing four mbt domains

Annotation Symbol

CG16975

Feature Type

protein_coding_gene

FlyBase ID

FBgn0032475

Gene Model Status

Current

Stock Availability

13 publicly available

Gene Summary

Scm-related gene containing four mbt domains (Sfmbt) encodes a subunit of the Polycomb Group protein complex Pho repressive complex (PhoRC). This chromatin binding protein recognizes with high selectivity mono- and dimethylated lysine residues in various histone peptides. [Date last reviewed: 2018-11-15] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

dSfmbt

Key Links

Genomic Location

Cytogenetic map

34A7-34A8

Sequence location

2L:13,169,649..13,176,785 [-]

Recombination map

2-47

RefSeq locus

NT_033779 REGION:13169649..13176785

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (16 terms)

Molecular Function (8 terms)

Terms Based on Experimental Evidence (6 terms)

CV Term

Evidence

References

enables chromatin binding

inferred from direct assay

(Gambetta et al., 2009)

enables histone H3K4me1 reader activity

inferred from direct assay

(Klymenko et al., 2006)

enables histone H3K9me2/3 reader activity

inferred from direct assay

(Klymenko et al., 2006)

enables histone H4K20me1 reader activity

inferred from direct assay

(Grimm et al., 2009, Klymenko et al., 2006)

enables histone H4K20me2 reader activity

inferred from direct assay

(Klymenko et al., 2006)

enables protein binding

inferred from physical interaction with FLYBASE:Scm; FB:FBgn0003334

(Grimm et al., 2009)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables chromatin binding

inferred from biological aspect of ancestor with PANTHER:PTN000261772

(GO Reference Genome Project, 2011-)

enables histone binding

inferred from biological aspect of ancestor with PANTHER:PTN000261772

(GO Reference Genome Project, 2011-)

enables zinc ion binding

inferred from electronic annotation with InterPro:IPR012313

(InterPro Project Members, 2004-)

Biological Process (6 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

involved_in heterochromatin formation

inferred from genetic interaction with FLYBASE:Scm; FB:FBgn0003334

(Grimm et al., 2009)

inferred from direct assay

(Klymenko et al., 2006)

involved_in imaginal disc growth

inferred from mutant phenotype

(Klymenko et al., 2006)

involved_in negative regulation of gene expression

inferred from mutant phenotype

(Lu et al., 2007)

involved_in oogenesis

inferred from mutant phenotype

(Klymenko et al., 2006)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in heterochromatin formation

inferred from electronic annotation with InterPro:IPR037605

(InterPro Project Members, 2004-)

involved_in negative regulation of DNA-templated transcription

inferred from biological aspect of ancestor with PANTHER:PTN000261772

(GO Reference Genome Project, 2011-)

involved_in regulation of DNA-templated transcription

inferred from electronic annotation with InterPro:IPR004092

(InterPro Project Members, 2004-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in nucleus

inferred from direct assay

(Klymenko et al., 2006)

part_of PcG protein complex

inferred from physical interaction with FLYBASE:pho; FB:FBgn0002521

(Klymenko et al., 2006)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000261772

(GO Reference Genome Project, 2011-)

located_in nucleus

inferred from electronic annotation with InterPro:IPR004092, InterPro:IPR012313

(InterPro Project Members, 2004-)

Gene Group (FlyBase)

PHO REPRESSIVE COMPLEX - PHO VARIANT

PHO REPRESSIVE COMPLEX - PHOL VARIANT

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

PHO REPRESSIVE COMPLEX - PHO VARIANT -: In Drosophila, the Pho repressive complex (PhoRC) can bind Polycomb Response Elements (PREs) and recruit Polycomb repressive complexes. (Adapted from FBrf0190547 and FBrf0220020).
PHO REPRESSIVE COMPLEX - PHOL VARIANT -: In Drosophila, the Pho repressive complex (PhoRC) can bind Polycomb Response Elements (PREs) and recruit Polycomb repressive complexes. (Adapted from FBrf0190547 and FBrf0220020).

Protein Function (UniProtKB)

Polycomb group (PcG) protein that binds to the Polycomb response elements (PREs) found in the regulatory regions of many genes. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. They probably act via the methylation of histones, rendering chromatin heritably changed in its expressibility. Necessary but not sufficient to recruit a functional PcG repressive complex that represses target genes, suggesting that the recruitment of the distinct PRC1 complex is also required to allow a subsequent repression.

(UniProt, Q9VK33)

Summary (Interactive Fly)

PcG protein - Pleiohomeotic interactor that binds mono- and di-methylated H3-K9 and H4-K20 and acts to maintain a Polycomb-repressed chromatin state

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Sfmbt for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VK33)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

PDB - An information portal to biological macromolecular structures

Comments on Gene Model

Gene model reviewed during 5.46

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Sfmbt-RA

FBtr0080491

NM_165026

3939

868

Sfmbt-RB

FBtr0080492

NM_135762

4777

1220

Sfmbt-RC

FBtr0289981

NM_001144349

4846

1243

Sfmbt-RD

FBtr0333236

NM_001273503

4765

1220

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Sfmbt-PA

FBpp0080069

95.9

868

5.51

NP_723786

AAF53250

Sfmbt-PB

FBpp0080070

133.7

1220

5.17

FBpp0288419

136.0

1243

5.42

FBpp0305438

133.7

1220

5.17

Q9VK33

NP_001260432

AGB92967

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1220 aa isoforms: Sfmbt-PB, Sfmbt-PD

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Interacts with pho as a component of the pho-repressive complex (PhoRC).

(UniProt, Q9VK33)

Domain

MBT repeats have unique discriminatory binding activity for methylated Lys residues in H3 and H4; the MBT repeats bind mono- and dimethylated H3K9Me1, H3K9Me2, H4K20Me1 and H4K20Me2 but fail to interact with these residues if they are unmodified or trimethylated.

(UniProt, Q9VK33)

Crossreferences

InterPro - A database of protein families, domains and functional sites

PDB - An information portal to biological macromolecular structures

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Sfmbt using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.51

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | faint | ubiquitous

(Fisher et al., 2012)

ventral ectoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

embryonic central brain neuron

(Fisher et al., 2012)

lateral cord neuron

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

embryonic Malpighian tubule

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

larval nervous system

(Fisher et al., 2012)

larval ventral nerve cord

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in nucleus

inferred from direct assay

(Klymenko et al., 2006)

part_of PcG protein complex

inferred from physical interaction with FLYBASE:pho; FB:FBgn0002521

(Klymenko et al., 2006)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Sfmbt in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Sfmbt

View exonic expression by tissue for Dmel\Sfmbt

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0032475

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34709/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0032475

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0032475

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0032475

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG16975

Flygut - An atlas of the Drosophila adult midgut

FBgn0032475

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Sfmbt

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 12 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 17 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Sfmbt

Transgenic constructs containing regulatory region of Sfmbt

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(2L)Exel8029

(Ryder, 2004.4.26)

Inferred from location ( 7 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Df(2L)BSC30/Sfmbt¹

Sfmbt^{αTub84B.Tag:TAP}

lethal, with Sfmbt¹

Sfmbt^{αTub84B.Tag:TAP}

lethal, with Sfmbt^{αTub84B.Tag:TAP}

Sfmbt¹

lethal (with Df(2L)BSC30), with Sfmbt^{αTub84B.Tag:TAP}

Sfmbt¹

lethal - all die before end of larval stage

Sfmbt¹

lethal - all die before end of larval stage (with Df(2L)BSC30)

Sfmbt¹

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Sfmbt^GD9249

viable, with Scer\GAL4^tin.CΔ4

Sfmbt^GD9249

Other Phenotypes

Allele

some die during third instar larval stage

Sfmbt¹

some die during third instar larval stage (with Df(2L)BSC30)

Sfmbt¹

Phenotype manifest in

Allele

female germline stem cell

Sfmbt¹

imaginal disc

Sfmbt¹

imaginal disc (with Df(2L)BSC30)

Sfmbt¹

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (19)

Hsap\MBTD1

11 of 14

Yes

Hsap\L3MBTL2

10 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Yes

Hsap\SFMBT2

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (16)

Rnor\L3mbtl2

10 of 14

Yes

Rnor\Mbtd1

10 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

Yes

Rnor\Sfmbt2

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (17)

Mmus\Mbtd1

11 of 14

Yes

Mmus\L3mbtl2

10 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Yes

Mmus\Sfmbt2

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (16)

Xtro\mbtd1

9 of 13

Yes

Xtro\lancl3

8 of 13

Yes

Xtro\l3mbtl2

6 of 13

Yes

Xtro\rasl12

2 of 13

Xtro\fmo3

1 of 13

Xtro\hcrt

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\spg21

1 of 13

Yes

Danio rerio (Zebrafish) (19)

Drer\l3mbtl2

10 of 14

Yes

Drer\mbtd1

10 of 14

Yes

Drer\l3mbtl1b

3 of 14

Drer\scml4

3 of 14

Drer\sfmbt2

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

Drer\LOC101883783

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Drer\si:ch211-230g15.5

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (4)

Cele\lin-61

7 of 14

Yes

Cele\mbtr-1

7 of 14

Yes

Cele\sor-3

1 of 14

Yes

Cele\tir-1

1 of 14

Anopheles gambiae (African malaria mosquito) (6)

Agam\AgaP_AGAP010202

11 of 12

Yes

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Sfmbt. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (7)

Scm

3 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

MBTD1; mbt domain containing 1

11 of 14

618705

L3MBTL2; L3MBTL histone methyl-lysine binding protein 2

10 of 14

611865

L3MBTL1; L3MBTL histone methyl-lysine binding protein 1

3 of 14

608802

L3MBTL4; L3MBTL histone methyl-lysine binding protein 4

3 of 14

617135

SCMH1; Scm polycomb group protein homolog 1

3 of 14

616396

SCML2; Scm polycomb group protein like 2

3 of 14

300208

SCML4; Scm polycomb group protein like 4

3 of 14

SFMBT1; Scm like with four mbt domains 1

3 of 14

607319

SFMBT2; Scm like with four mbt domains 2

3 of 14

615392

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Sfmbt

pull down, autoradiography

(Stielow et al., 2008)

Sfmbt - HDAC1

anti tag coimmunoprecipitation, peptide massfingerprinting

(Alfieri et al., 2013)

Sfmbt - HP1b

anti tag coimmunoprecipitation, peptide massfingerprinting, western blot

(Alfieri et al., 2013)

Sfmbt - His3

fluorescence polarization spectroscopy, isothermal titration calorimetry

(Grimm et al., 2009, Klymenko et al., 2006)

Sfmbt - His4

isothermal titration calorimetry, fluorescence polarization spectroscopy, x-ray crystallography

(Alfieri et al., 2013, Grimm et al., 2009, Klymenko et al., 2006)

Sfmbt - MEP-1

pull down, autoradiography

(Stielow et al., 2008)

Sfmbt - Mi-2

pull down, autoradiography

(Stielow et al., 2008)

Sfmbt - Nap1

anti tag coimmunoprecipitation, peptide massfingerprinting, western blot

(Alfieri et al., 2013)

Sfmbt - Pc

pull down, peptide massfingerprinting, western blot, tandem affinity purification, Identification by mass spectrometry

(Kang et al., 2015, Strübbe et al., 2011)

Sfmbt - Scm

tandem affinity purification, Identification by mass spectrometry, x-ray crystallography, molecular sieving, molecular weight estimation by staining, anti tag coimmunoprecipitation, western blot, pull down

(Frey et al., 2016, Kang et al., 2015, Grimm et al., 2009)

Sfmbt - Sumo

pull down, autoradiography

(Stielow et al., 2008)

Sfmbt - ocm

anti tag coimmunoprecipitation, peptide massfingerprinting

(Alfieri et al., 2013)

Sfmbt - ph-d

anti tag coimmunoprecipitation, peptide massfingerprinting, western blot

(Frey et al., 2016)

Sfmbt - ph-p

anti tag coimmunoprecipitation, peptide massfingerprinting, molecular weight estimation by staining

(Frey et al., 2016)

Sfmbt - pho

isothermal titration calorimetry, anti bait coimmunoprecipitation, western blot, pull down, molecular weight estimation by staining, anti tag coimmunoprecipitation, anti tag western blot, x-ray crystallography, peptide massfingerprinting, surface plasmon resonance, molecular sieving

(Seif and Francis, 2024, Pereira and Paro, 2017, Alfieri et al., 2013, Strübbe et al., 2011, Klymenko et al., 2006)

Sfmbt - phol

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, molecular weight estimation by staining

(Klymenko et al., 2006)

Sfmbt - rept

anti tag coimmunoprecipitation, peptide massfingerprinting

(Alfieri et al., 2013)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

rpr

enhanceable

Sfmbt

(Harris et al., 2020)

External Data

Subunit Structure (UniProtKB)

Interacts with pho as a component of the pho-repressive complex (PhoRC).

(UniProt, Q9VK33 )

Linkouts

BioGRID - A database of protein and genetic interactions.

60747

DroID - A comprehensive database of gene and protein interactions.

FBgn0032475

MIST (protein-protein) - An integrated Molecular Interaction Database

34709

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Polycomb repressive complex

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q9VK33

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-47

Cytogenetic map

34A7-34A8

Sequence location

2L:13,169,649..13,176,785 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

34A7-34A8

Limits computationally determined from genome sequence between P{lacW}l(2)k11328^k11328&P{lacW}l(2)k10105^k10105 and P{EP}EP850&P{lacW}l(2)k05911^k05911

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-47

(FlyBase, 2016)

2-43.6

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (13)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

28677

y¹ v¹; P{TRiP.JF03092}attP2

28439

y¹ w^*; P{EP}Sfmbt^G2280/CyO

604702

y¹ sc^* v¹ sev²¹; P{TOE.GS02907}attP40

32473

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00473}attP2/TM3, Sb¹

91568

y^d2 w¹¹¹⁸ P{ey-FLP.N}2; Sfmbt¹⁶⁴ P{neoFRT}40A/CyO; P{PRE.Scr-PS1}J3 P{PRE.Scr-PS1}Q1

NIG-Fly

16975R-3

P{NIG.16975R}3

M2L-1783

y¹ w¹¹¹⁸; Sfmbt^SK1 P{neoFRT}40A/CyO-Tb

M2L-1784

y¹ w¹¹¹⁸; Sfmbt^SK2 P{neoFRT}40A/CyO-Tb

2LG-1414

y² VhaAC39-1^cho-1 v¹; P{U6-Sfmbt.NIG.sgRNA}attP40/CyO

HMS00473

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00473}attP2/TM3, Sb¹

VDRC

v52247

w¹¹¹⁸; P{GD9249}v52247

v52249

w¹¹¹⁸; P{GD9249}v52249

More stocks available...

Genomic Clones (18)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (60)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD14884

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0032475

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0032475

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Erokhin et al., 2021, Erokhin et al., 2013, Klymenko et al., 2006)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

The 'PhoRC' (pho-Sfmbt) complex binds PREs via the pho protein and methylated histones in the chromatin flanking the PREs via the Sfmbt protein to maintain a repressed chromatin state.

(Klymenko et al., 2006)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: Sfmbt CG16975

(Klymenko et al., 2006)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

CG16975

(National Institute of Genetics Fly Stocks, 2022-, Ni et al., 2010.12.1, Perkins et al., 2009, Haussmann et al., 2008, Stielow et al., 2008, Lu et al., 2007, Klymenko et al., 2006)

SFMBT

(Richards et al., 2022, Ho et al., 2014, Kahn et al., 2014, Bonasio et al., 2010, Schwartz and Pirrotta, 2007)

Sfmbt

(Brown et al., 2024, Seif and Francis, 2024, Brown et al., 2023, Cabrita and Martinho, 2023, Erokhin et al., 2023, McPherson et al., 2023, Pang et al., 2023, Ding et al., 2022, Feijão et al., 2022, Fischer et al., 2022, Kang et al., 2022, Elizarev et al., 2021, Erokhin et al., 2021, Barbour et al., 2020, Chetverina et al., 2020, Ghotbi et al., 2020, Harris et al., 2020, Kennison, 2020.10.22, Onishi et al., 2020, Bonnet et al., 2019, Cheutin and Cavalli, 2019, Jiang et al., 2019, Alhaj Abed et al., 2018, Levis, 2018.8.30, Ma et al., 2018, Zhu et al., 2018, Monribot-Villanueva et al., 2017, Transgenic RNAi Project members, 2017-, Frey et al., 2016, Kahn et al., 2016, Bajusz et al., 2015, Erokhin et al., 2015, Kang et al., 2015, Gonzalez et al., 2014, McElroy et al., 2014, Alfieri et al., 2013, Aloia et al., 2013, Erokhin et al., 2013, Moshkin et al., 2013, Follmer et al., 2012, Islam et al., 2011, Kolaczkowski et al., 2011, Pruteanu-Malinici et al., 2011, Saj et al., 2010, Stielow et al., 2008, Grimm et al., 2007, Klymenko et al., 2006)

dSFMBT

(Zhimulev et al., 2024, Decourcelle et al., 2019, Rennie et al., 2018, Comoglio and Paro, 2014, Lanzuolo and Orlando, 2012, Moshkin et al., 2009)

dSfmbt

(Chetverina et al., 2019, Erceg et al., 2017, Monribot-Villanueva et al., 2017, Pereira and Paro, 2017, Na et al., 2015, Alic et al., 2011, Richter et al., 2011, Strübbe et al., 2011, Gambetta et al., 2009, Gambetta et al., 2009, Grimm et al., 2009, Huang et al., 2008, Oktaba et al., 2008, Lu et al., 2007, Klymenko et al., 2006, Muller and Kassis, 2006, Papp and Muller, 2006)

sfmbt

(DeLuca et al., 2020)

Name Synonyms

CG16975

Scm-related gene containing four mbt domains

(Comoglio and Paro, 2014, McElroy et al., 2014)

Secondary FlyBase IDs

Datasets (2)

Study focus (2)

Experimental Role

Project

Project Type

Title

bait_protein

modENCODE_chromatin_proteins_cells

genome binding

Genome-wide localization of chromosomal proteins in cell lines by ChIP-chip and ChIP-Seq.

bait_protein

modENCODE_chromatin_proteins_flies

genome binding

Genome-wide localization of chromosomal proteins in fly tissues by ChIP-chip and ChIP-Seq.

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 66 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34709

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VK33

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VK33

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VK33

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0032475

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34709/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0032475

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0032475

FlyMine - An integrated database for Drosophila genomics

FBgn0032475

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG16975

MARRVEL_MODEL - MARRVEL (model organism gene)

34709

PDB - An information portal to biological macromolecular structures

Linkouts

BioGRID - A database of protein and genetic interactions.

60747

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0032475

DroID - A comprehensive database of gene and protein interactions.

FBgn0032475

DRSC - Results frm RNAi screens

FBgn0032475

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0032475

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0032475

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG16975

Flygut - An atlas of the Drosophila adult midgut

FBgn0032475

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0032475

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC007651

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Polycomb repressive complex

MIST (protein-protein) - An integrated Molecular Interaction Database

34709

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q9VK33

References (114)

The following external sites may use different assemblies or annotations than FlyBase.

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