FB2026_03 , released September 17, 2026

CV Term Report

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General Information

Term

surface plasmon resonance

ID (Ontology)

MI:0107 (Molecular Interactions)

Definition

This method measures formation of complex by monitoring changes in the resonance angle of light impinging on a gold surface as a result of changes in the refractive index of the surface. A ligand of interest (small molecule, peptide, protein, sugar, lipid, nucleic acid) is immobilized on a gold surface, and the interacting partner is injected in buffer flow over it. Biomolecules that interact with the immobilized ligand are retained on the surface, and alter the resonance angle of impinging light as a result of the change in refractive index brought about by the increased biomolecule mass retained on the surface. Since all the biomolecules belonging to the same class have the same refractive index and since there is a linear correlation between resonance angle shift and biomolecule concentration near the surface, this allows one to measure changes in concentration at the surface as a consequence of interaction. Furthermore, this is done in real time, allowing direct measurement of both the on-rate and the off-rate and of the affinity constant of complex formation.[ PubMed:11896282 PubMed:12120258 PubMed:16338355 ]

Also Known As

"BIAcore(r)" ; "Optical biosensor" ; "spr"

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Full annotation statements	Relevant FlyBase reports
Full annotation statements	Interaction Groups
surface plasmon resonance (all annotations which use CV term, excluding "NOT" statements)

Spanning Tree (Parents/Children)

feature detection method
 |__experimental feature detection__
biophysical                         |
 |__biosensor_______________________|
                                    surface plasmon resonance  131 rec.
                                     |__surface plasmon resonance array

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Relationships
Is a	experimental feature detection biosensor
Part of
Synonyms & Secondary IDs
Synonyms
	"BIAcore(r)" EXACT PSI-MI-alternate "Optical biosensor" EXACT PSI-MI-alternate "spr" EXACT PSI-MI-short
Secondary IDs

External Crossreferences & Linkouts