FB2026_03 , released September 17, 2026

CV Term Report

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General Information

Term

protein three hybrid

ID (Ontology)

MI:0437 (Molecular Interactions)

Definition

Two hybrid assay performed with a third protein component co-transfected into a recombinant yeast strain together with a bait and a prey construct. Negative control shows that the interaction between the bait and the prey do not occur when the third protein is not co-transfected.[ PubMed:12052864 PubMed:12761205 PubMed:12935900 ]

Also Known As

"bridge assay" ; "protein 3-hybrid" ; "protein tri hybrid" (for all, see Synonyms field below)

Comment

Links to External Ontologies

Annotations

Records annotated with this term OR any of its CHILD TERMS

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Full annotation statements including this term (annotations to child terms are NOT included), and relevant FlyBase records

Full annotation statements	Relevant FlyBase reports
Full annotation statements	Interaction Groups
protein three hybrid (all annotations which use CV term, excluding "NOT" statements)

Spanning Tree (Parents/Children)

transcriptional complementation assay
 |__two hybrid____
 |__three hybrid__|
                  protein three hybrid  17 rec.

Spanning Tree View Settings
Parents/Children View Depth	Show hierarchy levels: for parents, for children
Relationships
Is a	two hybrid three hybrid
Part of
Synonyms & Secondary IDs
Synonyms
	"bridge assay" EXACT PSI-MI-alternate "protein 3-hybrid" EXACT PSI-MI-alternate "protein tri hybrid" EXACT PSI-MI-alternate "trihybrid" EXACT PSI-MI-alternate
Secondary IDs

External Crossreferences & Linkouts