FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Cenp-C, cid
FlyBase ID
FBig0000102196
Interaction Network
Interactions Browser links
Cenp-C network
cid network
Reported Interactions
FBrf0206297-2.coIP.WB
Reference
Erhardt et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc167
Participants
Corresponds to
Reported as
Role
Note
cid 

CID

bait

GFP tag

Cenp-C 

CENP-C

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Kc167 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Erhardt et al., 2008)

Chromatin was digested to primarily mono- and dinucleosomes with micrococcal nuclease prior to immunoprecipitation.

(Erhardt et al., 2008)
FBrf0206297-4.coIP.WB
Reference
Erhardt et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc167
Participants
Corresponds to
Reported as
Role
Note
Cenp-C 

CENP-C

bait

GFP tag

cid 

CID

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Kc167 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Erhardt et al., 2008)

Chromatin was digested to primarily mono- and dinucleosomes with micrococcal nuclease prior to immunoprecipitation.

(Erhardt et al., 2008)
FBrf0212150-3.Y3H
Reference
Schittenhelm et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cid 

cid

bait

fused to GAL4 DNA-binding domain

Cenp-C 

Cenp-C

prey

fused to GAL4 activation domain

Cenp-C 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cenp-C 
C-terminal domain
sufficient binding region
aa 1201-1411
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Schittenhelm et al., 2010)
FBrf0227269-10.coIP.MS
Reference
Lipinszki et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Cenp-C 

CENP-C

bait

Protein A tag

cid 

CENP-A/CID

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Lipinszki et al., 2015)
FBrf0243708-5.PBD.FM
Reference
Roure et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cenp-C 

dCENP-C

bait

GFP tag

cid 

dCENP-A

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The ability of Cenp-C fused to LacI to recruit cid to LacO arrays was taken as evidence of a Cenp-C-cid interaction. Co-expression of cal1 strongly enhances the Cenp-C-cid interaction.

(Roure et al., 2019)

Source was U2OS cells; bait produced from transfected construct; prey produced from transfected construct.

(Roure et al., 2019)
FBrf0243708-7.PBD.FM
Reference
Roure et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cid 

dCENP-A

bait

GFP tag

Cenp-C 

dCENP-C

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The ability of cid fused to LacI to recruit Cenp-C to LacO arrays was taken as evidence of a Cenp-C-cid interaction.

(Roure et al., 2019)

Source was U2OS cells; bait produced from transfected construct; prey produced from transfected construct.

(Roure et al., 2019)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)