Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

Interaction between Spt6 and an in vitro produced and reconstituted cid/His4 tetramer captured as two binary interactions.

The Spt6-cid interaction persists in 750mM NaCl.

Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from transfected construct.