FlyBase Allele Report: Dmel\elB[EP2039]

General Information

Symbol

Dmel\elB^EP2039

Species

D. melanogaster

Name

FlyBase ID

FBal0093970

Feature type

allele

Associated gene

Dmel\elB

Associated Insertion(s)

P{EP}elB^EP2039

Carried in Construct

Also Known As

EP(2)2039

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Roote, 1998.11.16)

Mutagen

P-element activity

(Dorfman et al., 2002, Tseng and Hariharan, 2002, Gene Disruption Project members, 2001-, Abdelilah-Seyfried et al., 2000, Ashburner et al., 1999, Ashburner et al., 1999, Ashburner et al., 1999.10.12, Roote, 1998.11.16)

Progenitor genotype

Associated Insertion(s)

P{EP}elB^EP2039

Cytology

Description

P{EP} insertion 965bp upstream of the 5' UTR and 1365bp upstream of the translation start site of elB.

(Dorfman et al., 2002)

P{EP} insertion at the 5' end of the predicted BG:DS06238.3 gene.

(Ashburner et al., 1999)

P{EP} insertion at position 45657 of DS06238.

(Ashburner et al., 1999.10.12)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:14,409,608..14,409,608 [-]

(Levine et al., 2007, Weihe et al., 2004, Dorfman et al., 2002, Tseng and Hariharan, 2002, Gene Disruption Project members, 2001-, Abdelilah-Seyfried et al., 2000, Ashburner et al., 1999, Ashburner et al., 1999, Ashburner et al., 1999.10.12, Roote, 1998.11.16)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with Scer\GAL4^btl.PS

(Dorfman et al., 2002)

lethal, with Scer\GAL4^en-e16E

(Tseng and Hariharan, 2002)

viable

(Dorfman et al., 2002)

visible, with Scer\GAL4^ey.PH

(Tseng and Hariharan, 2002)

visible | recessive

(Ashburner et al., 1999)

Phenotype Manifest In

dorsal tracheal branch primordium, with Scer\GAL4^btl.PS

(Dorfman et al., 2002)

eye, with Scer\GAL4^ey.PH

(Tseng and Hariharan, 2002)

tracheal transverse connective primordium, with Scer\GAL4^btl.PS

(Dorfman et al., 2002)

visceral tracheal branch primordium, with Scer\GAL4^btl.PS

(Dorfman et al., 2002)

Detailed Description

Statement

Reference

The visceral branch of the tracheal system is not formed in most segments in embryos expressing elB^EP2039 under the control of Scer\GAL4^btl.PS. The cells which normally form the visceral branch remain in the central region of the transverse connective branch. The dorsal branch generally contains more cells than normal. The number of cells in the lateral trunk anterior branch normal in these embryos.

(Dorfman et al., 2002)

Expression of elB^EP2039 under the control of Scer\GAL4^ey.PH results in eyes that are strongly reduced in size. Flies expressing elB^EP2039 under the control of Scer\GAL4^hs.2sev have wild-type eyes. Cells from third larval instar wing discs expressing elB^EP2039 under the control of Scer\GAL4^Act5C.PP are normal in size. There is a small but reproducible increase in the G2/M population.

(Tseng and Hariharan, 2002)

Weak allele.

(Ashburner et al., 1999)

Shows an "elbow" phenotype when hemizygous.

(Ashburner et al., 1999.10.12)

Shows a very weak elbow phenotype Df(2L)A217.

(Roote, 1998.11.16)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^ey.PH, elB^EP2039 has visible phenotype, suppressible by CycE^UAS.cLa, Scer\GAL4^ey.PH

(Tseng and Hariharan, 2002)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^ey.PH, elB^EP2039 has eye phenotype, suppressible by CycE^UAS.cLa, Scer\GAL4^ey.PH

(Tseng and Hariharan, 2002)

Enhancer of

Statement

Reference

elB^EP2039 is an enhancer of phenotype of sna^Sco

(Ashburner et al., 1999)

Other

Statement

Reference

Scer\GAL4^btl.PS, elB^EP2039, tkv^{Q253D.UAS.cLa} has tracheal lateral trunk anterior branch primordium phenotype

(Dorfman et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of both elB^EP2039 and tkv^{Q253D.Scer\UAS.cLa} under the control of Scer\GAL4^btl.PS results in an excess of lateral trunk anterior branch cells in the embryo.

(Dorfman et al., 2002)

Co-expression of CycE^Scer\UAS.cLa suppresses the small eye phenotype caused by expression of elB^EP2039 under the control of Scer\GAL4^ey.PH. Cells from third larval instar wing discs expressing elB^EP2039 under the control of Scer\GAL4^Act5C.PP (and also co-expressing BacA\p35^Scer\UAS.cHa to eliminate cell death) have a 26% longer doubling time than control cells expressing BacA\p35^Scer\UAS.cHa alone under the control of Scer\GAL4^Act5C.PP.

(Tseng and Hariharan, 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Excisions of the P{EP} element can result in reversion of the elB phenotype.

(Ashburner et al., 1999)

Excision of the P{EP} element can revert the mutant phenotype.

(Ashburner et al., 1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

EP(2)2039

(Abdelilah-Seyfried et al., 2000, Ashburner et al., 1999, Ashburner et al., 1999.10.12)

elB^EP2039

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

Open Close