FlyBase Allele Report: Dmel\esg[EP684]

General Information

Symbol

Dmel\esg^EP684

Species

D. melanogaster

Name

FlyBase ID

FBal0089285

Feature type

allele

Associated gene

Dmel\esg

Associated Insertion(s)

P{EP}esg^EP684

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Rorth et al., 1998)

Progenitor genotype

Associated Insertion(s)

P{EP}esg^EP684

Cytology

Description

P{EP} insertion 443bp upstream of the esg translation initiation codon. The P{EP} element is oriented such that expression from the element should result in overexpression of esg.

(Bidet et al., 2003)

P{EP} insertion at +564bp of the transcription unit.

(Abdelilah-Seyfried et al., 2000)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:15,333,648..15,333,648 [+]

(Cao et al., 2008, Stofanko et al., 2008, Levine et al., 2007, Hekmat-Scafe et al., 2005, Bidet et al., 2003, Pena-Rangel et al., 2002, Tseng and Hariharan, 2002, Gene Disruption Project members, 2001-, Abdelilah-Seyfried et al., 2000, Ashburner et al., 1999, Ashburner et al., 1999.10.12, Rorth et al., 1998)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Alzheimer's disease

modeled by Hsap\APP^{Aβ42.GMR.Tag:SS(rPENK)}

(Cao et al., 2008)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with Scer\GAL4^hs.2sev

(Tseng and Hariharan, 2002)

visible, with Scer\GAL4^en-e16E

(Tseng and Hariharan, 2002)

visible, with Scer\GAL4^ey.PH

(Tseng and Hariharan, 2002)

visible, with Scer\GAL4^sca-537.4

(Abdelilah-Seyfried et al., 2000)

visible | dominant, with Scer\GAL4^hs.2sev

(Rorth et al., 1998)

Phenotype Manifest In

embryonic/larval hemocyte | third instar larval stage, with Scer\GAL4^Pxn.PS

(Stofanko et al., 2008)

eye, with Scer\GAL4^ey.PH

(Tseng and Hariharan, 2002)

eye, with Scer\GAL4^hs.2sev

(Rorth et al., 1998)

macrochaeta & scutum, with Scer\GAL4^sca-537.4

(Abdelilah-Seyfried et al., 2000)

microchaeta & scutum, with Scer\GAL4^sca-537.4

(Abdelilah-Seyfried et al., 2000)

thecogen cell, with Scer\GAL4^sca-537.4

(Abdelilah-Seyfried et al., 2000)

wing, with Scer\GAL4^en-e16E

(Tseng and Hariharan, 2002)

Detailed Description

Statement

Reference

Expression using Scer\GAL4^ey.PU does not induce a rough eye phenotype.

(Cao et al., 2008)

Third instar larvae expressing esg^EP684 under the control of Scer\GAL4^Pxn.PS have an increased number of sessile hemocytes. The number of hemocytes in circulation is also increased. The dorsal sessile hemocyte department is disrupted. Sessile hemocytes do not accumulate along the dorsal vessel but spread through the cuticle. The lymph gland is enlarged. Hemocyte shape is normal.

(Stofanko et al., 2008)

Expression of esg^EP684 under the control of Scer\GAL4^ey.PH results in eyes that are slightly reduced in size. Flies expressing esg^EP684 under the control of Scer\GAL4^en-e16E have scalloped wings.

(Tseng and Hariharan, 2002)

Expression of esg^EP684 under the control of Scer\GAL4^sca-537.4 results in complete loss of all macrochaetae and microchaetae on the notum, and no sheath cells.

(Abdelilah-Seyfried et al., 2000)

No obvious phenotype.

(Ashburner et al., 1999)

Expression of esg^EP684 under the control of Scer\GAL4^hs.2sev results in small glassy eyes.

(Rorth et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Scer\GAL4^ey.PH, esg^EP684 has visible phenotype, non-suppressible by CycE^UAS.cLa, Scer\GAL4^ey.PH

(Tseng and Hariharan, 2002)

Suppressor of

Statement

Reference

esg^EP684/Scer\GAL4^elav.PLu is a suppressor of bang sensitive phenotype of para^bss1

(Hekmat-Scafe et al., 2005)

esg^EP684/Scer\GAL4^ChAT.7.4 is a suppressor of bang sensitive phenotype of eas²

(Hekmat-Scafe et al., 2005)

Scer\GAL4^G14/esg^EP684 is a suppressor of bang sensitive phenotype of eas²

(Hekmat-Scafe et al., 2005)

esg^EP684/Scer\GAL4^elav.PLu is a suppressor of abnormal neurophysiology phenotype of eas²

(Hekmat-Scafe et al., 2005)

esg^EP684/Scer\GAL4^elav.PLu is a suppressor of bang sensitive phenotype of eas²

(Hekmat-Scafe et al., 2005)

esg^EP684/Scer\GAL4^elav.PLu is a suppressor of bang sensitive phenotype of jus^iso7.8

(Hekmat-Scafe et al., 2005)

NOT Suppressor of

Statement

Reference

Scer\GAL4^RapGAP1-OK6/esg^EP684 is a non-suppressor of bang sensitive phenotype of eas²

(Hekmat-Scafe et al., 2005)

Other

Statement

Reference

Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^GMR.PU, esg^EP684 has lethal phenotype

(Cao et al., 2008)

Hsap\MAPT^UAS.cWa, Scer\GAL4^ey.PU, esg^EP684 has lethal phenotype

(Cao et al., 2008)

Scer\GAL4^pnr-MD237, esg^EP684, pnr^MD237/pnr[+] has lethal | embryonic stage phenotype

(Pena-Rangel et al., 2002)

Scer\GAL4^pnr-MD237, esg^EP684, pnr^MD237 has lethal | embryonic stage phenotype

(Pena-Rangel et al., 2002)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Scer\GAL4^ey.PH, esg^EP684 has eye phenotype, non-suppressible by CycE^UAS.cLa, Scer\GAL4^ey.PH

(Tseng and Hariharan, 2002)

Enhancer of

Statement

Reference

esg^EP684/Scer\GAL4^ey.PU is an enhancer of eye phenotype of Hsap\APP^{Aβ42.GMR.Tag:SS(rPENK)}

(Cao et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

The bang sensitivity of bss¹/+ flies (more than 50% of flies are bang sensitive) is substantially suppressed by the expression of esg^EP684 under the control of Scer\GAL4^elav.PLu. However, the 100% bang sensitivity of bss¹/bss¹ flies is not suppressed by the expression of esg^EP684 under the control of Scer\GAL4^elav.PLu. The 100% bang sensitivity of eas²/Y flies can be suppressed by expression of esg^EP684 under the control of Scer\GAL4^elav.PLu (flies have 4% bang sensitivity), Scer\GAL4^Cha.7.4 (flies have 88% bang sensitivity) or Scer\GAL4^G14 (flies have 78% bang sensitivity), but it not suppressed by expression of esg^EP684 under the control of Scer\GAL4^OK6. The seizure sensitivity of eas² flies is partially suppressed by expression of esg^EP684 under the control of Scer\GAL4^elav.PLu; the mean seizure threshold is raised to 15.5 +/- 5.7 V (for a high frequency stimulus of 0.5msec pulses delivered at 200Hz for 300 msec).

(Hekmat-Scafe et al., 2005)

Co-expression of CycE^Scer\UAS.cLa does not suppress the small eye phenotype caused by expression of esg^EP684 under the control of Scer\GAL4^ey.PH.

(Tseng and Hariharan, 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Selected as: a P{EP} insertion line that modifies the pnr^MD237/+ phenotype when expressed using Scer\GAL4^pnr-MD237.

(Pena-Rangel et al., 2002)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

EP(2)0684

(Abdelilah-Seyfried et al., 2000, Ashburner et al., 1999.10.12)

esg^EP684

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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